LOCUS       sequence-385697-        2220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, 
            BsmBI, and BbsI Type IIS restriction enzymes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pGGAselect
FEATURES             Location/Qualifiers
     source          1..2220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        254..272
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     254..271
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(463..482)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(464..482)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(593..1252)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1167..1186
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1253..1355)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      1456..2044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1945..1964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2198..2215
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt
       61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg
      121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag
      181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg
      241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt
      301 cgaagtcttg gtacggagcg agaccggagc gagacgggag tcgtcttcgc tttccagatc
      361 tgataacttg tgaagacgac catcgtctca ccatggtctc accattcctg tagacttctt
      421 aattaagacg tcagaattct cgaggcggcc gcatgtgagt ctccctatag tgagtcgtat
      481 taatttcgcg ggcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
      541 gctcatgagt agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
      601 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      661 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      721 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      781 aatcaaaact ggtgaaactc acccagggat tggctgagac aaaaaacata ttctcaataa
      841 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      901 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      961 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1021 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1081 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1141 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1201 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1261 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1321 tatggtgaaa gttggaacct cttacgtgcc gatcaaagtc tcattttcgc caaaagttgt
     1381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1561 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2041 gaaaaacgcc agcaatgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
//
