LOCUS       sequence-385707-       11979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GFP-RELA K5R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Jeremiah N, Ferran H, Antoniadou K, De Azevedo K, Nikolic J, Maurin 
            M, Benaroch P, Manel N
  TITLE     RELA tunes innate-like interferon I/III responses in human T cells.
  JOURNAL   J Exp Med. 2023 May 1;220(5):e20220666. doi: 10.1084/jem.20220666. 
            Epub 2023 Feb 23.
  PUBMED    36820829
REFERENCE   2  (bases 1 to 11979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2023-05-1"; volume: "220(5):e20220666. doi"; pages: " 
            10.1084/jem.20220666. Epub 2023 Feb 23"
COMMENT     Sequence Label: pTRIP-SFFV-GFP-RELA K5R
FEATURES             Location/Qualifiers
     source          1..11979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      3736..3745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3742..3744,3745..3747,3748..4455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(3742..3744,3745..3747,3748..4455)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3787..3808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5740..6111
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     LTR             6383..6563
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8091..8110)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8324..8343)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8443..8465
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8503..8521)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8589..8693
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8694..8762,8763..9554)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8912..8931)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9725..10313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10214..10233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10467..10484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10554..10574)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10559..10888
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10739..10874
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcggcc gcgggctagc
     3721 ggatccccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3841 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     3901 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     3961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4201 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4261 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacacaat ggacgaactg ttccccctca tcttcccggc agagccagcc
     4501 caggcctctg gcccctatgt ggagatcatt gagcagccca agcagcgggg catgcgcttc
     4561 cgctacaagt gcgaggggcg ctccgcgggc agcatcccag gcgagaggag cacagatacc
     4621 accaagaccc accccaccat caagatcaat ggctacacag gaccagggac agtgcgcatc
     4681 tccctggtca ccaaggaccc tcctcaccgg cctcaccccc acgagcttgt aggaaaggac
     4741 tgccgggatg gcttctatga ggctgagctc tgcccggacc gctgcatcca cagtttccag
     4801 aacctgggaa tccagtgtgt gagaaggcgg gacctggagc aggctatcag tcagcgcatc
     4861 cagaccaaca acaacccctt ccaagttcct atagaagagc agcgtgggga ctacgacctg
     4921 aatgctgtgc ggctctgctt ccaggtgaca gtgcgggacc catcaggcag gcccctccgc
     4981 ctgccgcctg tcctttctca tcccatcttt gacaatcgtg cccccaacac tgccgagctc
     5041 aagatctgcc gagtgaaccg aaactctggc agctgcctcg gtggggatga gatcttccta
     5101 ctgtgtgaca aggtgcagaa agaggacatt gaggtgtatt tcacgggacc aggctgggag
     5161 gcccgaggct ccttttcgca agctgatgtg caccgacaag tggccattgt gttccggacc
     5221 cctccctacg cagaccccag cctgcaggct cctgtgcgtg tctccatgca gctgcggcgg
     5281 ccttccgacc gggagctcag tgagcccatg gaattccagt acctgccaga tacagacgat
     5341 cgtcaccgga ttgaggagaa acgtaaaagg acatatgaga ccttcagaag catcatgaga
     5401 aggagtcctt tcagcggacc caccgacccc cggcctccac ctcgacgcat tgctgtgcct
     5461 tcccgcagct cagcttctgt ccccaagcca gcaccccagc cctatccctt tacgtcatcc
     5521 ctgagcacca tcaactatga tgagtttccc accatggtgt ttccttctgg gcagatcagc
     5581 caggcctcgg ccttggcccc ggcccctccc caagtcctgc cccaggctcc agcccctgcc
     5641 cctgctccag ccatggtatc agctctggcc caggccccag cccctgtccc agtcctagcc
     5701 ccaggccctc ctcaggctgt ggccccacct gcccccaagc ccacccaggc tggggaagga
     5761 acgctgtcag aggccctgct gcagctgcag tttgatgatg aagacctggg ggccttgctt
     5821 ggcaacagca cagacccagc tgtgttcaca gacctggcat ccgtcgacaa ctccgagttt
     5881 cagcagctgc tgaaccaggg catacctgtg gccccccaca caactgagcc catgctgatg
     5941 gagtaccctg aggctataac tcgcctagtg acaggggccc agaggccccc cgacccagct
     6001 cctgctccac tgggggcccc ggggctcccc aatggcctcc tttcaggaga tgaagacttc
     6061 tcctccattg cggacatgga cttctcagcc ctgctgagtc agatcagctc ctaactcgag
     6121 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6181 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6241 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6301 gactggaagg gctaattcac tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa
     6361 gcagctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6421 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6481 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6541 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6601 acttgcaaag aaatgaatat cagagagtga gaggccttga cattataata gatttagcag
     6661 gaattgaact aggagtggag cacacaggca aagctgcaga agtacttgga agaagccacc
     6721 agagatactc acgattctgc acatacctgg ctaatcccag atcctaagga ttacattaag
     6781 tttactaaca tttatataat gatttatagt ttaaagtata aacttatcta atttactatt
     6841 ctgacagata ttaattaatc ctcaaatatc ataagagatg attactatta tccccattta
     6901 acacaagagg aaactgagag ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc
     6961 gttagttacg actctatgat cttctgacac aaattccatt tactcctcac cctatgactc
     7021 agtcgaatat atcaaagtta tggacattat gctaagtaac aaattaccct tttatatagt
     7081 aaatactgag tagattgaga gaagaaattg tttgcaaacc tgaatagctt caagaagaag
     7141 agaagtgagg ataagaataa cagttgtcat ttaacaagtt ttaacaagta acttggttag
     7201 aaagggattc aaatgcataa agcaagggat aaatttttct ggcaacaaga ctatacaata
     7261 taaccttaaa tatgacttca aataattgtt ggaacttgat aaaactaatt aaatattatt
     7321 gaagattatc aatattataa atgtaattta cttttaaaaa gggaacatag aaatgtgtat
     7381 cattagagta gaaaacaatc cttattatca caatttgtca aaacaagttt gttattaaca
     7441 caagtagaat actgcattca attaagttga ctgcagattt tgtgttttgt taaaattaga
     7501 aagagataac aacaatttga attattgaaa gtaacatgta aatagttcta catacgttct
     7561 tttgacatct tgttcaatca ttgatcgaag ttctttatct tggaagaatt tgttccaaag
     7621 actctgaaat aaggaaaaca atctattata tagtctcaca cctttgtttt acttttagtg
     7681 atttcaattt aataatgtaa atggttaaaa tttattcttc tctgagatca tttcacattg
     7741 cagatagaaa acctgagact ggggtaattt ttattaaaat ctaatttaat ctcagaaaca
     7801 catctttatt ctaacatcaa tttttccagt ttgatattat catataaagt cagccttcct
     7861 catctgcagg ttccacaaca aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa
     7921 ttaaaaatag caatacaaca ataaaaaaat acaaatcaga aaaacagcac agtataacaa
     7981 ctttatttag catttacaat ctattaggta ttataagtaa tctagccaga tcctctacgc
     8041 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     8101 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     8161 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     8221 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     8281 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     8341 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     8401 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     8461 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     8521 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     8581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8641 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8701 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8761 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8821 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8881 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8941 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     9001 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     9061 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     9121 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     9181 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     9241 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     9301 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     9361 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     9421 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     9481 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9541 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9601 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9661 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9721 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9781 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9841 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9901 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9961 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    10021 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    10081 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    10141 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    10201 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    10261 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    10321 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    10381 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    10441 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    10501 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
    10561 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10621 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
    10681 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
    10741 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
    10801 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10861 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
    10921 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
    10981 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
    11041 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
    11101 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
    11161 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
    11221 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
    11281 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
    11341 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
    11401 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
    11461 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
    11521 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
    11581 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
    11641 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
    11701 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
    11761 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
    11821 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
    11881 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
    11941 ccccggatct ttgtgaagga accttacttc tgtggtgtg
//
