LOCUS       sequence-385710-       11560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express Zebrasfish STING.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Jeremiah N, Ferran H, Antoniadou K, De Azevedo K, Nikolic J, Maurin 
            M, Benaroch P, Manel N
  TITLE     RELA tunes innate-like interferon I/III responses in human T cells.
  JOURNAL   J Exp Med. 2023 May 1;220(5):e20220666. doi: 10.1084/jem.20220666. 
            Epub 2023 Feb 23.
  PUBMED    36820829
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2023-05-1"; volume: "220(5):e20220666. doi"; pages: " 
            10.1084/jem.20220666. Epub 2023 Feb 23"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A-ZebrafishSTING
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             5964..6144
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7672..7691)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7905..7924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8024..8046
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8084..8102)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8170..8274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8275..8343,8344..9135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8493..8512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9306..9894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9795..9814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10048..10065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10135..10155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10140..10469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10320..10455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc ctctgtgatg
     4501 ggagaagacg ctctcgtccc cagagcgcgc agcaggctgc cggtgatgtg tgctgctgga
     4561 ctgggttttc ttactctggc cgttgcttgg ctgctggact cagacaagtt cagtgaaaga
     4621 gctggaatta tcgcttttgg gctcatgctg gaaaggttta tttactgtat atgtttgtta
     4681 gcagaggaat tgctcttcca ttcaaggcaa aggtatcatg gcagaatgag tgagattttc
     4741 cgagcttgct ttagagggag tggcattctg ggaatgtgtg caatattcct gatgctcatg
     4801 ttgggtggag tttccttttc cgtgaagcag tggagccact tcaacctcat gtgcgccgga
     4861 tacatgttgc tcaatagcct gggagtgctg ggcccagctc cagtcgagat ttcagaaata
     4921 tgtgaagcaa aaaagatgaa cgtggctcat ggtctggcct ggtctttcta tatcggctac
     4981 ctcaaatttc tccttccagc tttagaggtg aacgtcagag aatactctag aagggaacga
     5041 ctgagttctc cacgtctaca tatccttctg cccctcaatg ccagagtccc aagcaaacct
     5101 gaagaggagg acacgaatgt ggtcttccat gaaaaccttc cagatctgaa gctggacagg
     5161 gcaggagtgc ggaaacgcag ctacactaac agcgtctaca agatcaccca caacaatgag
     5221 acgtttagct gcattttgga atatgccaca ccgctgctga cgctctatca gatgtcccag
     5281 gagagcagtg cagggtttgg cgagagagaa cggaagcagc aggtcctgct gttctataga
     5341 accctcagcc aaattctgga caattctctg gagtgtcgga accggtaccg gctcatcctg
     5401 ctcaacgatg agcacacagg tgatcctcat tacctctcca gagagctctt ccagaacctg
     5461 aagcagcagg atggggagat tttcatggac ccaaccaatg aagtccaccc agttccagaa
     5521 gagggtccgg ttgggaactg taatggcgca ctgcaagcca cttttcatga agagccaatg
     5581 agcgacgagc ccaccctcat gttcagccga cctcaatccc taagatccga gcctgtggag
     5641 accactgatt attttaaccc atctagcgca atgaaacaaa actgataagc ggccgctcga
     5701 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5761 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5821 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5881 ggactggaag ggctaattca ctcccaacga agacaaaatc gtcgagagat gctgcatata
     5941 agcagctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6001 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6061 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6121 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6181 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat agatttagca
     6241 ggaattgaac taggagtgga gcacacaggc aaagctgcag aagtacttgg aagaagccac
     6301 cagagatact cacgattctg cacatacctg gctaatccca gatcctaagg attacattaa
     6361 gtttactaac atttatataa tgatttatag tttaaagtat aaacttatct aatttactat
     6421 tctgacagat attaattaat cctcaaatat cataagagat gattactatt atccccattt
     6481 aacacaagag gaaactgaga gggaaagatg ttgaagtaat tttcccacaa ttacagcatc
     6541 cgttagttac gactctatga tcttctgaca caaattccat ttactcctca ccctatgact
     6601 cagtcgaata tatcaaagtt atggacatta tgctaagtaa caaattaccc ttttatatag
     6661 taaatactga gtagattgag agaagaaatt gtttgcaaac ctgaatagct tcaagaagaa
     6721 gagaagtgag gataagaata acagttgtca tttaacaagt tttaacaagt aacttggtta
     6781 gaaagggatt caaatgcata aagcaaggga taaatttttc tggcaacaag actatacaat
     6841 ataaccttaa atatgacttc aaataattgt tggaacttga taaaactaat taaatattat
     6901 tgaagattat caatattata aatgtaattt acttttaaaa agggaacata gaaatgtgta
     6961 tcattagagt agaaaacaat ccttattatc acaatttgtc aaaacaagtt tgttattaac
     7021 acaagtagaa tactgcattc aattaagttg actgcagatt ttgtgttttg ttaaaattag
     7081 aaagagataa caacaatttg aattattgaa agtaacatgt aaatagttct acatacgttc
     7141 ttttgacatc ttgttcaatc attgatcgaa gttctttatc ttggaagaat ttgttccaaa
     7201 gactctgaaa taaggaaaac aatctattat atagtctcac acctttgttt tacttttagt
     7261 gatttcaatt taataatgta aatggttaaa atttattctt ctctgagatc atttcacatt
     7321 gcagatagaa aacctgagac tggggtaatt tttattaaaa tctaatttaa tctcagaaac
     7381 acatctttat tctaacatca atttttccag tttgatatta tcatataaag tcagccttcc
     7441 tcatctgcag gttccacaac aaaaatccaa ccaactgtgg atcaaaaata ttgggaaaaa
     7501 attaaaaata gcaatacaac aataaaaaaa tacaaatcag aaaaacagca cagtataaca
     7561 actttattta gcatttacaa tctattaggt attataagta atctagccag atcctctacg
     7621 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     7681 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     7741 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     7801 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     7861 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
     7921 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     7981 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     8041 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     8101 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     8161 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8221 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8281 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8341 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8401 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8461 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8521 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8581 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8641 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8701 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8761 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8821 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8881 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8941 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9001 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9061 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9121 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9181 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
    10141 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
    10201 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
    10261 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10321 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10381 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
    10441 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
    10501 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
    10561 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
    10621 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
    10681 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
    10741 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
    10801 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
    10861 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
    10921 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
    10981 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
    11041 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
    11101 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
    11161 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
    11221 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
    11281 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
    11341 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
    11401 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
    11461 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
    11521 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg
//
