LOCUS       sequence-385721-       13822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of full length mouse Lepr with eGFP
            marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13822)
  AUTHORS   Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg 
            TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E
  TITLE     Ras drives malignancy through stem cell crosstalk with the 
            microenvironment.
  JOURNAL   Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6.
  PUBMED    36450983
REFERENCE   2  (bases 1 to 13822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Nov 30. doi: 10.1038/s41586-022-05475-6."
COMMENT     Sequence Label: TRE-Lepr_FL-IRES-eGFP
FEATURES             Location/Qualifiers
     source          1..13822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    join(2067..2085,2086..2108,2109..2127,2128..2150,2151..2169,
                     2170..2192,2193..2211,2212..2234,2235..2253,2254..2276,
                     2277..2295,2296..2318,2319..2337)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2370..2408
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2405..2429
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    2600..2717
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6256..6823
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6274..6825
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6418..6435)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6645..6664
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6828..6830,6831..6833,6834..7544)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6828..6830,6831..6833,6834..7544)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6873..6894)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7134..7153)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7481..7502
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7623..7639)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7624..7640)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7653..8241
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7706..7726)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8124..8135)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8385..8418)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8438..8618
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8643..8661)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8644..8661)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8951..8970)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9184..9203)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9303..9325
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9363..9381)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9449..9553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9554..9622,9623..10414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9772..9791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10585..11173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11074..11093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11327..11344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11414..11434)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11419..11748
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11599..11734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12882..12947
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13077..13097
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13522..13656
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13559..13578)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13613..13632
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcta gagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2281 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2341 gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa
     2401 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2461 tccatagaag acaccgggac cgatccagcc tccgcggccc cgaattgtga gatctcaacc
     2521 ggtttaggcg gccgcttctg ctagctaatt cgagctcggt acccggggat cctctagaat
     2581 ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg
     2641 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     2701 caaaaattca aaattttatc gatcacgaga ctagcctcga ggtttaaacc gccaccatga
     2761 tgtgtcagaa attctatgtg gttttgttac actgggaatt tctttatgtg atagctgcac
     2821 ttaacctggc atatccaatc tctccctgga aatttaagtt gttttgtgga ccaccgaaca
     2881 caaccgatga ctcctttctc tcacctgctg gagccccaaa caatgcctcg gctttgaagg
     2941 gggcttctga agcaattgtt gaagctaaat ttaattcaag tggtatctac gttcctgagt
     3001 tatccaaaac agtcttccac tgttgctttg ggaatgagca aggtcaaaac tgctctgcac
     3061 tcacagacaa cactgaaggg aagacactgg cttcagtagt gaaggcttca gtttttcgcc
     3121 agctaggtgt aaactgggac atagagtgct ggatgaaagg ggacttgaca ttattcatct
     3181 gtcatatgga gccattacct aagaacccct tcaagaatta tgactctaag gtccatcttt
     3241 tatatgatct gcctgaagtc atagatgatt cgcctctgcc cccactgaaa gacagctttc
     3301 agactgtcca atgcaactgc agtcttcggg gatgtgaatg tcatgtgccg gtacccagag
     3361 ccaaactcaa ctacgctctt ctgatgtatt tggaaatcac atctgccggt gtgagttttc
     3421 agtcacctct gatgtcactg cagcccatgc ttgttgtgaa acccgatcca cccttaggtt
     3481 tgcatatgga agtcacagat gatggtaatt taaagatttc ttgggacagc caaacaatgg
     3541 caccatttcc gcttcaatat caggtgaaat atttagagaa ttctacaatt gtaagagagg
     3601 ctgctgaaat tgtctcagct acatctctgc tggtagacag tgtgcttcct ggatcttcat
     3661 atgaggtcca ggtgaggagc aagagactgg atggttcagg agtctggagt gactggagtt
     3721 cacctcaagt ctttaccaca caagatgttg tgtattttcc acccaaaatt ctgactagtg
     3781 ttggatcgaa tgcttctttt cattgcatct acaaaaacga aaaccagatt atctcctcaa
     3841 aacagatagt ttggtggagg aatctagctg agaaaatccc tgagatacag tacagcattg
     3901 tgagtgaccg agttagcaaa gttaccttct ccaacctgaa agccaccaga cctcgaggga
     3961 agtttaccta tgacgcagtg tactgctgca atgagcaggc gtgccatcac cgctatgctg
     4021 aattatacgt gatcgatgtc aatatcaata tatcatgtga aactgacggg tacttaacta
     4081 aaatgacttg cagatggtca cccagcacaa tccaatcact agtgggaagc actgtgcagc
     4141 tgaggtatca caggcgcagc ctgtattgtc ctgatagtcc atctattcat cctacgtctg
     4201 agcccaaaaa ctgcgtctta cagagagacg gcttttatga atgtgttttc cagccaatct
     4261 ttctattatc tggctataca atgtggatca ggatcaacca ttctttaggt tcacttgact
     4321 cgccaccaac gtgtgtcctt cctgactccg tagtaaaacc actacctcca tctaacgtaa
     4381 aagcagagat tactgtaaac actggattat tgaaagtatc ttgggaaaag ccagtctttc
     4441 cggagaataa ccttcaattc cagattcgat atggcttaag tggaaaagaa atacaatgga
     4501 agacacatga ggtattcgat gcaaagtcaa agtctgccag cctgctggtg tcagacctct
     4561 gtgcagtcta tgtggtccag gttcgctgcc ggcggttgga tggactagga tattggagta
     4621 attggagcag tccagcctat acgcttgtca tggatgtaaa agttcctatg agagggcctg
     4681 aattttggag aaaaatggat ggggacgtta ctaaaaagga gagaaatgtc accttgcttt
     4741 ggaagcccct gacgaaaaat gactcactgt gtagtgtgag gaggtacgtg gtgaagcatc
     4801 gtactgccca caatgggacg tggtcagaag atgtgggaaa tcggaccaat ctcactttcc
     4861 tgtggacaga accagcgcac actgttacag ttctggctgt caattccctc ggcgcttccc
     4921 ttgtgaattt taaccttacc ttctcatggc ccatgagtaa agtgagtgct gtggagtcac
     4981 tcagtgctta tcccctgagc agcagctgtg tcatcctttc ctggacactg tcacctgatg
     5041 attatagtct gttatatctg gttattgaat ggaagatcct taatgaagat gatggaatga
     5101 agtggcttag aattccctcg aatgttaaaa agttttatat ccacgataat tttattccca
     5161 tcgagaaata tcagtttagt ctttacccag tatttatgga aggagttgga aaaccaaaga
     5221 taattaatgg tttcaccaaa gatgctatcg acaagcagca gaatgacgca gggctgtatg
     5281 tcattgtacc cataattatt tcctcttgtg tcctactgct cggaacactg ttaatttcac
     5341 accagagaat gaaaaagttg ttttgggacg atgttccaaa ccccaagaat tgttcctggg
     5401 cacaaggact gaatttccaa aagcctgaaa catttgagca tctttttacc aagcatgcag
     5461 aatcagtgat atttggtcct cttcttctgg agcctgaacc catttcagaa gaaatcagtg
     5521 tcgatacagc ttggaaaaat aaagatgaga tggtcccagc agctatggtc tcccttcttt
     5581 tgaccacacc agaccctgaa agcagttcta tttgtattag tgaccagtgt aacagtgcta
     5641 acttctctgg gtctcagagc acccaggtaa cctgtgagga tgagtgtcag agacaaccct
     5701 cagttaaata tgcaactctg gtcagcaacg ataaactagt ggaaactgat gaagagcaag
     5761 ggtttatcca tagtcctgtc agcaactgca tctccagtaa tcattcccca ctgaggcagt
     5821 ctttctctag cagctcctgg gagacagagg cccagacatt tttcctttta tcagaccagc
     5881 aacccaccat gatttcacca caactttcat tctcggggtt ggatgagctt ttggaactgg
     5941 agggaagttt tcctgaagaa aatcacaggg agaagtctgt ctgttatcta ggagtcacct
     6001 ccgtcaacag aagagagagt ggtgtgcttt tgactggtga ggcaggaatc ctgtgcacat
     6061 tcccagccca gtgtctgttc agtgacatca ggatcctcca ggagagatgc tcacactttg
     6121 tagaaaataa tttgagttta gggacctctg gtgagaactt tgtaccttac atgccccaat
     6181 ttcaaacctg ttccacgcac agtcacaaga taatggagaa taagatgtgt gacttaactg
     6241 tgtaactgca ggaattccgc cccccccccc ctaacgttac tggccgaagc cgcttggaat
     6301 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     6361 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     6421 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     6481 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     6541 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     6601 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     6661 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6721 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     6781 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taataccatg gtgagcaagg
     6841 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     6901 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     6961 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     7021 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     7081 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     7141 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     7201 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     7261 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     7321 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     7381 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     7441 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     7501 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     7561 gactagctag tagctagcta gctagtcgag ctcaagcttc gaattcgata tcaagcttat
     7621 cgcgataccg tcgacctcga gggaattccg ataatcaacc tctggattac aaaatttgtg
     7681 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7741 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7801 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     7861 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     7921 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     7981 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8041 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8101 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8161 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8221 ccctttgggc cgcctccccg catcgggaat tcgagctcgg tacctttaag accaatgact
     8281 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     8341 attcactccc aacgaagaca agatgggatc aattcaccat gggaataact tcgtatagca
     8401 tacattatac gaagttatgc tgctttttgc ttgtactggg tctctctggt tagaccagat
     8461 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8521 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8581 cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt
     8641 attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag
     8701 ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag
     8761 accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga
     8821 gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc
     8881 gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg
     8941 gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg
     9001 ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg
     9061 ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta
     9121 ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct
     9181 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     9241 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     9301 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     9361 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     9421 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9481 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9541 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9601 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9661 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9721 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9781 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9841 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9901 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9961 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    10021 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    10081 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    10141 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    10201 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10261 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10321 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10381 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    10441 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10501 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    10561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    11041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    11101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    11161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    11221 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11281 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11341 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11401 ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    11461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    11521 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    11581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    11701 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac
    11761 aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag
    11821 tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa
    11881 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
    11941 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
    12001 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
    12061 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
    12121 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
    12181 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
    12241 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
    12301 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
    12361 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
    12421 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
    12481 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
    12541 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
    12601 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
    12661 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
    12721 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
    12781 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
    12841 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    12901 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    12961 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
    13021 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
    13081 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
    13141 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
    13201 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
    13261 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
    13321 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
    13381 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
    13441 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
    13501 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
    13561 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    13621 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    13681 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    13741 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    13801 atatgtacta caaacttagt ag
//
