LOCUS       sequence-385725-        4496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mouse cGAS protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Jneid B, Bochnakian A, Hoffmann C, Delisle F, Djacoto E, Sirven P, 
            Denizeau J, Sedlik C, Gerber-Ferder Y, Fiore F, Akyol R, Brousse C, 
            Kramer R, Walters I, Carlioz S, Salmon H, Malissen B, Dalod M, 
            Piaggio E, Manel N
  TITLE     Selective STING stimulation in dendritic cells primes antitumor T 
            cell responses.
  JOURNAL   Sci Immunol. 2023 Jan 13;8(79):eabn6612. doi: 
            10.1126/sciimmunol.abn6612. Epub 2023 Jan 13.
  PUBMED    36638189
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-13"; volume: "8(79):eabn6612. doi"; pages:
            " 10.1126/sciimmunol.abn6612. Epub 2023 Jan 13"
COMMENT     Sequence Label: pVAX1-cGAS
FEATURES             Location/Qualifiers
     source          1..4496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        36..415
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        416..619
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     570..590
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     664..683
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        664..682
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      721..730
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2321..2338)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2327..2551
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             2724..3518
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2724..3518
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2778..2797)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3388..3407
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      3843..4431
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4332..4351
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
       61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
      661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttgcc
      721 gccaccatgg aggatcccag gaggaggact acagctccaa gggccaagaa gccctcagcc
      781 aaacgagctc caacacagcc tagcagaaca cgcgctcatg ctgagtcttg tggcccacaa
      841 cgcggagcga gaagccgtag ggcagaacgc gatggggata ccactgagaa gcccagggca
      901 ccaggtccga gagttcatcc agcacgggca acggaactga ccaaagacgc ccaaccgtct
      961 gccatggatg ctgcaggcgc cacagcgagg cctgcagtac gggtacccca gcagcaggcc
     1021 atcctggatc ctgaactgcc agctgtgaga gaacctcaac ctccagctga ccccgaagct
     1081 cgaaaagtgg tgagaggacc cagtcatcgg agaggggcga gatctactgg tcagcctcgc
     1141 gccccgagag gctccagaaa ggaacctgac aaactgaaga aagtgctgga taaactccgg
     1201 ctcaaacgta aagacattag tgaggctgcg gaaaccgtga acaaggtggt tgagcggctg
     1261 ttgaggagaa tgcagaagcg agagagcgag ttcaaagggg tggaacaact gaatacggga
     1321 tcctattatg aacacgtcaa aataagtgcc ccgaatgagt ttgacgtgat gttcaagctt
     1381 gaagtcccac gcatcgagct gcaggagtac tacgaaaccg gagccttcta tctcgttaaa
     1441 ttcaagcgga ttccacgggg taatcctctg agccactttc tcgagggcga ggttttgtct
     1501 gcaacaaaga tgctgtcaaa gtttcgtaaa atcatcaagg aagaggtaaa ggagattaag
     1561 gacatcgatg tgagcgtgga gaaagaaaag cctggaagtc cagcagtgac tctgctgatc
     1621 aggaatcccg aagaaatttc agtcgatatc attctggccc ttgagagcaa ggggtcttgg
     1681 cctatatcta ccaaagaggg cttgcctatt cagggttggc tcggcaccaa ggtcaggacc
     1741 aacttgcggc gcgaaccctt ctatctcgtc cccaagaatg ccaaggacgg gaacagcttt
     1801 caaggcgaga catggcggtt gagcttttcc catacggaga aatacattct gaacaatcac
     1861 gggattgaga aaacttgctg tgagagtagc ggagccaaat gttgtcgcaa agaatgcctg
     1921 aagctgatga agtacctgtt ggaacagctt aagaaagagt ttcaggagct ggatgccttc
     1981 tgctcatatc acgtcaagac tgccatcttt cacatgtgga cacaggatcc acaggactca
     2041 cagtgggatc cccgcaacct ttcctcctgc tttgacaagc tcctggcttt cttcctggaa
     2101 tgccttcgta ccgagaagct cgaccactac tttatcccca aattcaacct gttctcccag
     2161 gaactgatag accgaaagtc caaagagttc ctgagcaaga agatagagta cgagcgaaac
     2221 aatggcttcc ccatctttga caaactttga gaattctgca gatatccagc acagtggcgg
     2281 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     2341 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     2401 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     2461 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     2521 aggcatgctg gggatgcggt gggctctatg gcttctactg ggcggtttta tggacagcaa
     2581 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa
     2641 actggatggc tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag
     2701 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     2761 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     2821 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     2881 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     2941 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3001 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3061 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3121 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3181 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3241 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3301 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3361 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3421 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3481 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tattaacgct tacaatttcc
     3541 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     3601 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3661 tatccgctca tgagacaata accctgataa atgcttcaat aatagcacgt gctaaaactt
     3721 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     3781 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     3841 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     3901 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     3961 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4021 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4081 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4141 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     4201 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     4261 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     4321 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     4381 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     4441 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctt
//
