LOCUS       sequence-385728-        2052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid carries a fragment from a 24-fragment lacI/lacZ 
            cassette split that was designed as a Golden Gate assembly with high
            fidelity..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Potapov V, Ong JL, Kucera RB, Langhorst BW, Bilotti K, Pryor JM, 
            Cantor EJ, Canton B, Knight TF, Evans TC Jr, Lohman GJS
  TITLE     Comprehensive Profiling of Four Base Overhang Ligation Fidelity by 
            T4 DNA Ligase and Application to DNA Assembly.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2665-2674. doi: 
            10.1021/acssynbio.8b00333. Epub 2018 Oct 29.
  PUBMED    30335370
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00333"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2665-2674"
COMMENT     Sequence Label: pUC57-mini v2-HF24_F4
FEATURES             Location/Qualifiers
     source          1..2052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(297..885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(377..396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1056..1847,1848..1916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1679..1698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1917..2021)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatgg tctcgggtcg taatcagcac cgcatcagca agtgtatctg ccgtgcactg
       61 caacaacgct gcttcggcct ggtaatggcc cgccgccttc cagcgttcga cccaggcgtt
      121 agggtcaatg cgggtcgctt cacttacgcc aatgtcgtta tccagcggtg cacgggtgaa
      181 ctgatcgcgc agcggcgtca gcagttgttt tttatcgccg agaccatcgg aaagaacatg
      241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagat
     1201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2041 ccacctgacg tc
//
