LOCUS       sequence-38573-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: LCMV:GFP(FRT)MCS
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..96
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             112..828
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(142..170)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     765..785
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 caggggcttt agcatgctcg ttagggaacc gccattctgc ctggggacgt cggagcaagc
       61 ttgaagttcc tattctctag aaagtatagg aacttcggat ccacagccac catgagtaaa
      121 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      181 gggtacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      241 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      301 ctcacttatg gtgttcaatg cttttcaaga tatccagatc atatgaagcg gcacgacttc
      361 ttcaagagcg ccatgcctga gggatacgtg caggagagga ccatcttctt caaggacgac
      421 gggaactaca agacacgtgc tgaagtcaag tttgagggag acaccctcgt caacaggatc
      481 gagcttaagg gaatcgattt caaggaggac ggaaacatcc tcggccacaa gttggaatac
      541 aactacaact cccacaacgt atacatcatg gccgacaagc aaaagaacgg catcaaagcc
      601 aacttcaaga cccgccacaa catcgaagac ggcggcgtgc aactcgctga tcattatcaa
      661 caaaatactc caattggcga tgaccctgtc cttttaccag acaaccatta cctgtccaca
      721 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      781 gtaacggctg ctgggattac acatggcatg gatgaactat acaaataact cgagcctcta
      841 gaggatcata atcagccata acccaccatt ttg
//
