LOCUS       sequence-38574-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-oriT
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(191..295)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     363..381
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(385..428)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     oriT            480..589
                     /note="incP origin of transfer"
ORIGIN
        1 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
       61 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      121 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      181 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
      241 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
      301 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
      361 aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg
      421 atgatttctg gaattcatcc tgaacttatc tagacttggt gtatccaacg gcgtcagccg
      481 ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat
      541 tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc taccgccggc
      601 gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa gggcagccca
      661 cctatcacgg aattgatccc cctcgaattg acgcgtcaat tctcatgttt gacagcttat
      721 catcgataag ctagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
      781 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
      841 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcggga
//
