LOCUS       sequence-385741-        3510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cCadC-CCB3.5 under the control of PBAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Lee B, Wang T
  TITLE     A Modular Scaffold for Controlling Transcriptional Activation via 
            Homomeric Protein-Protein Interactions.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3198-3206. doi: 
            10.1021/acssynbio.2c00501. Epub 2022 Oct 10.
  PUBMED    36215660
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00501"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3198-3206"
COMMENT     Sequence Label: pBL090J
FEATURES             Location/Qualifiers
     source          1..3510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(82..627)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(162..181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(739..1617)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1644..1928
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1851..1870
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             complement(2636..3451)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2636..3451)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3359..3378
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3443..3462
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ctacggatcg gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga
       61 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
      121 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      181 ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc
      241 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
      301 gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg
      361 gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca
      421 ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa
      481 aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta
      541 gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagat
      601 attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaaggagcc ccatacgata
      661 tacgagagga catccgggca aatgcaacga aaggctcagt cgaaagactg ggcctttcgt
      721 tgcagactta gggacccttt atgacaactt gacggctaca tcattcactt tttcttcaca
      781 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata
      841 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
      901 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
      961 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     1021 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     1081 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     1141 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     1201 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     1261 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     1321 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     1381 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     1441 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     1501 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     1561 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     1621 tttcatactc ccaccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     1681 tcactgcgtc ttttactggc tcttctcgct aacccaaccg gtaaccccgc ttattaaaag
     1741 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     1801 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     1861 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     1921 ttctccatac ccgttttttt acctgcaggt gcagtaaagg aaaaaaaaaa tgcaacaacc
     1981 tgtagttcgc gttggcgaat ggcttgttac tccgtccata aaccaaatta gccgcaatgg
     2041 gcgtcaactt acccttcgtc cgagattaat cgatcttctg gttttctttg ctcaacacag
     2101 tggcgaagta cttagcaggg atgaacttat cgataatgtc tggaagagaa gtattgtcac
     2161 caatcacgtt gtgacgcaga gtatctcaga actacgtaag tcattaaaag ataatgatga
     2221 agatagtcct gtctatatcg ctactgtacc aaagcgcggc tataaattaa tggtgccggt
     2281 tatctggtac agcgaagaag agggagagga aataatgcta tcttcggcag cggctatctc
     2341 tgaggcggtt cctgccacag attctccctc ccacagtctt aacattcaaa acaccgcaac
     2401 gccacctgaa caatccccag ttaaaagcaa acgaggtgga tctggtggtc tgaaacagaa
     2461 gatcgccgca ctgaaataca agaatgcggc cctgaaaaag aagatcgcgg cactgaaaca
     2521 gggttaaatt aacggcactg cgaaaaaacc ccgccgaagc ggggtttttt gcgaaatata
     2581 atgaccctct tgataaccca agagggcatt ttttaatgcc catggcgtac ggatcttaga
     2641 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     2701 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     2761 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     2821 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     2881 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     2941 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     3001 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     3061 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3121 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     3181 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3241 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     3301 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     3361 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     3421 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     3481 cagacagttt tattgttcat gaagagaaac
//
