LOCUS       sequence-385748-        5421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PCadBA3 expressing LuxAB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Lee B, Wang T
  TITLE     A Modular Scaffold for Controlling Transcriptional Activation via 
            Homomeric Protein-Protein Interactions.
  JOURNAL   ACS Synth Biol. 2022 Oct 21;11(10):3198-3206. doi: 
            10.1021/acssynbio.2c00501. Epub 2022 Oct 10.
  PUBMED    36215660
REFERENCE   2  (bases 1 to 5421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00501"; journalName: "ACS Synth Biol"; date: 
            "2022-10-21- 21"; volume: "11"; issue: "10"; pages: "3198-3206"
COMMENT     Sequence Label: pTW160C
FEATURES             Location/Qualifiers
     source          1..5421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(14..964)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1012..1234
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             2160..3239
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3303..4283
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
                     SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
                     SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
                     PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
                     HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
                     AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
     CDS             complement(join(4363..5154,5155..5223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQDQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4986..5005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5224..5328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5396..5414
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccaaataggc cgttcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt
       61 gtttttgcgt gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca
      121 aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt
      181 ttcttagtcc gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat
      241 tcatttttat ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa
      301 cttggaaaat caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc
      361 tgtaagtgtt taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact
      421 catggtagtt attttcaagc attaacatga acttaaattc atcaaggcta atctctatat
      481 ttgccttgtg agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag
      541 agtatttgtt ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact
      601 ggaaaagata aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact
      661 tggcatagtt tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca
      721 cagttctcgt catcagctct ctggttgctt tagctaatac accataagca ttttccctac
      781 tgatgttcat catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg
      841 tagggttttc aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat
      901 gctccgttaa gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag
      961 acatacatct caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc
     1021 aatgataatt actagtcctt ttcctttgag ttgtgggtat ctgtaaattc tgctagacct
     1081 ttgctggaaa acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg
     1141 ttttttttgt ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga
     1201 taaaaagaat agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt
     1261 acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag
     1321 gcttaagtag caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc
     1381 atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg
     1441 gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta
     1501 cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta
     1561 tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga
     1621 ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc
     1681 ggtatcatca acaggcttac ccgtcttact gtcaagagga catccggtca aataaaacga
     1741 aaggctcagt cgaaagactg ggcctttcgt tttgctgagg agacttaggg accctgtaac
     1801 tccgggttga tttatgctcg gaaatatttg ttgttgagtt tttgtatgtt cctgttggta
     1861 taatatgttg cggcaattta tttgccgcat aatttttatt acataaattt aaccagagaa
     1921 tgtcacgcaa tccattgtaa acattaaatg tttatctttt catgatatca acttgcgatc
     1981 ctgatgtgtt aataaaaaac ctcaagttct cacttacaga aacttttgtg ttatttcacc
     2041 taatctttag gattaatcct tttttcgtga gtaatcttat cgccagtttg gtctggtcag
     2101 gaaatagtta tacatcatga cccggactcc aaattcaaaa atgaaattag gagaagagca
     2161 tgaaatttgg aaactttttg cttacatacc aacctcccca attttcccaa acagaggtaa
     2221 tgaaacgttt ggttaaatta ggtcgcatct ctgaggagtg tggttttgat accgtatggt
     2281 tactggagca tcatttcacg gagtttggtt tgcttggtaa cccttatgtc gctgctgcat
     2341 atttacttgg cgcgactaaa aaattgaatg taggaactgc cgctattgtt cttcccacag
     2401 cccatccagt acgccaactt gaagatgtga atttattgga tcaaatgtca aaaggacgat
     2461 ttcggtttgg tatttgccga gggctttaca acaaggactt tcgcgtattc ggcacagata
     2521 tgaataacag tcgcgcctta gcggaatgct ggtacgggct gataaagaat ggcatgacag
     2581 agggatatat ggaagctgat aatgaacata tcaagttcca taaggtaaaa gtaaaccccg
     2641 cggcgtatag cagaggtggc gcaccggttt atgtggtggc tgaatcagct tcgacgactg
     2701 agtgggctgc tcaatttggc ctaccgatga tattaagttg gattataaat actaacgaaa
     2761 agaaagcaca acttgagctt tataatgaag tggctcaaga atatgggcac gatattcata
     2821 atatcgacca ttgcttatca tatataacat ctgtagatca tgactcaatt aaagcgaaag
     2881 agatttgccg gaaatttctg gggcattggt atgattctta tgtgaatgct acgactattt
     2941 ttgatgattc agaccaaaca agaggttatg atttcaataa agggcagtgg cgtgactttg
     3001 tattaaaagg acataaagat actaatcgcc gtattgatta cagttacgaa atcaatcccg
     3061 tgggaacgcc gcaggaatgt attgacataa ttcaaaaaga cattgatgct acaggaatat
     3121 caaatatttg ttgtggattt gaagctaatg gaacagtaga cgaaattatt gcttccatga
     3181 agctcttcca gtctgatgtc atgccatttc ttaaagaaaa acaacgttcg ctattatatt
     3241 atggcggtgg cggtagcggc ggtggcggta gcggcggtgg cggtagcggc ggtggcggta
     3301 gcaaatttgg attgttcttc cttaacttca tcaattcaac aactgttcaa gaacagagta
     3361 tagttcgcat gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag
     3421 tgtatgaaaa tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt
     3481 ttctgctcgg tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc
     3541 atcatcctgt ccgcatagcg gaggaagctt gcttattgga tcagttaagt gaagggagat
     3601 ttattttagg gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg
     3661 ttgaatatca acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa
     3721 caggctattg taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc
     3781 atgcttatac gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg
     3841 agtgggcggc caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta
     3901 gatatgaata tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag
     3961 agatagacca tcagttaatg atattagtta actataacga agatagtaat aaagctaaac
     4021 aagagacgcg tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg
     4081 aaaataaact tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa
     4141 ctgcggctaa gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac
     4201 caatgaatga tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaga
     4261 agtaccacac ggaatatacc taaacttaat taacggcact cctcagcaaa tataatgacc
     4321 ctcttgataa cccaagaggg cattttttaa tgcccatggc gtttaccaat gcttaatcag
     4381 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     4441 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     4501 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     4561 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     4621 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     4681 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     4741 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     4801 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     4861 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     4921 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgatcttgcc cggcgtcaat
     4981 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5041 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     5101 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     5161 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     5221 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     5281 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     5341 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     5401 gcgtatcacg aggcccttag g
//
