LOCUS       sequence-385775-       10705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3451
FEATURES             Location/Qualifiers
     source          1..10705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3459..3483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3491..3532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3643..4231
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3696..3716)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4114..4125)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4303..4536
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4614..4735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4651..4670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4705..4724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4775..4910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4930..4949)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4931..4949)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4959..4976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4959..4975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4968..4990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5117..5572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5204..5223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5414..5435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5598..5702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5703..5771,5772..6563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5921..5940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6734..7322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7223..7242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7476..7493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7610..7631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7646..7651,7652..7669,7670..7676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7684..7700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7689..7711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7708..7724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7708..7724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7743..7763
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7745..7763
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7791..8017
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8018..8198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8245..8370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8863..9096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9281..9325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9556..9766
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9578..9774
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9625..9760
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9806..10408
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9806..9825)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10302..10322
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10451..10568
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcaccatgca gaaggaaatg aagatgatca aagacgagga
     1261 tgtgcatttc gacttggctg tgaagaagac cccctccttt ccccactgcc tgcagccagt
     1321 ggcttctcgg ggaaaggctc cccaaagaca ccccttcccg gaagctctcc gagggccatt
     1381 ttcccagttt cggtatgaac ctcccccagg agacctagat gggttccccg gggtcttcga
     1441 aggagcaggg tctaggaaac ggaagagcat gcccacaaag atgccctata accaccctgc
     1501 agaagaagtc accctcgccc tccactcaga ggagaacaaa aaccacggcc ttcccaacct
     1561 ccctttgctg ttcccgcagc ccccgcgccc caagtatgac tctcagatga tcgacctgtg
     1621 caacgtgggc ttccaattct accgcagcct ggaacacttt gggggcaagc ccgtcaagca
     1681 ggaacccatt aagcccagcg ccgtgtggcc ccagccaacg cccactccat tcctgcccac
     1741 gccctacccc tactacccca aagtccaccc gggcctcatg ttccccttct tcgtgccctc
     1801 gtcctcgccc ttccccttca gccggcacac cttcctgccc aagcagcccc cggaacctct
     1861 gctgccccgg aaagccgagc cccaggagag cgaggagacc aagcagaagg tggagagggt
     1921 ggacgtgaac gtgcagatcg atgacagcta ctacgtggac gtgggcggct cgcagaagcg
     1981 ctggcagtgc cccacctgcg agaagtccta cacctccaag tacaacctgg tcacccacat
     2041 cctgggccac agtgggatca agccgcacgc gtgcacgcac tgcgggaagc tcttcaagca
     2101 gctcagccac ctgcataccc acatgctgac ccaccagggc acgcggcccc acaagtgcca
     2161 ggtgtgccac aaggccttca cccagaccag ccacctgaag cgccacatga tgcagcacag
     2221 cgaggtgaag ccgcacaact gccgcgtgtg cggccgcggc tttgcctacc ccagcgagct
     2281 caaggcccac gaagccaagc acgccagtgg gcgcgagaac atctgtgtgg agtgcggcct
     2341 cgacttcccc accttggccc agctgaagag acacctcacc acgcaccggg gccccatcca
     2401 gtacaactgc tccgagtgcg acaagacctt ccagtacccg agccagctgc agaaccacat
     2461 gatgaagcac aaggacatcc ggccctacat ctgctcagag tgtggcatgg agtttgtgca
     2521 gccgcaccac ctcaagcagc actccctcac ccacaagggt gtgaaggagc ataagtgtgg
     2581 gatttgtggg cgggagttca ccctgctggc caacatgaag cgacacgtgc tgatccacac
     2641 caacatccgc gcctaccagt gtcacctctg ctacaagagc ttcgtgcaga agcagaccct
     2701 caaggcacac atgatcgtcc actctgacgt gaagcctttc aaatgcaagc tttgtgggaa
     2761 ggaattcaac cggatgcaca acctgatggg ccacatgcac ctgcactcag acagcaaacc
     2821 cttcaagtgc ctctattgcc caagcaaatt caccctgaag gggaacctga cacgccacat
     2881 gaaagtcaag catggagtca tggagcgggg ccttcattcc caaggtctgg gaagggggag
     2941 aatcgccctg gcacagacag ccggtgtcct gaggagtctg gagcaggagg agccctttga
     3001 cctctctcag aagcgccggg ccaaggtgcc ggtgttccag tcagacgggg agagtgccca
     3061 gggcagccac tgccacgagg aggaagagga ggataactgc tacgaggtgg agccctacag
     3121 ccctggcctg gccccccaga gccagcagct ctgcacaccc gaggatctgt ccaccaagtc
     3181 ggagcacgcc cccgaggtgc tggaggaagc ctgcaaggag gagaaggagg atgcatccaa
     3241 gggagaatgg gagaagagga gcaagggtga ccttggggca gagggcggcc aggagagaga
     3301 ctgtgccggc agagatgagt gtctcagtct cagggctttt cagagtaccc ggcggggccc
     3361 ctctttttct gattacttat acttcaagca cagagatgag agtttgaaag aattactgga
     3421 gaggaaaatg gaaaaacaag cagtgctttt aggtatcttg ccaactttct tgtacaaagt
     3481 ggttgatatc ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg
     3541 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     3601 cgactgtcga taagcgcggc atgaaccacg cgttaagtcg acaatcaacc tctggattac
     3661 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3721 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3781 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3841 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3901 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3961 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4021 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4081 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4141 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     4201 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     4261 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4321 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4381 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4441 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4501 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     4561 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4621 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4741 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4861 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     4921 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     4981 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5041 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5101 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     5161 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5221 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5281 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5341 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5401 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5461 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5521 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5581 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5641 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5701 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5761 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5821 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5881 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5941 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6001 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6061 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6121 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6181 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6241 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6301 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6361 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6421 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6481 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6541 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6601 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6661 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6721 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6781 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6841 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6901 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6961 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7081 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7261 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7321 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7381 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7441 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7501 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7561 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7621 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7681 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7741 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     7801 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     7861 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     7921 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7981 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8041 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8101 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8161 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     8221 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     8281 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8341 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8401 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8461 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8521 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8581 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8641 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8701 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8761 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8821 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8881 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8941 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9001 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9061 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9121 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9181 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     9241 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9301 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9361 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9421 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9481 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     9541 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     9601 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9661 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9721 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     9781 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     9841 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     9901 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     9961 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    10021 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    10081 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    10141 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    10201 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    10261 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    10321 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    10381 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    10441 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10501 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10561 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    10621 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    10681 ctaggtcttg aaaggagtgg gaatt
//
