LOCUS       sequence-385778-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0509
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4408..4996
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4461..4481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4879..4890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5068..5301
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5379..5500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5416..5435)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5470..5489
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5540..5675
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5695..5714)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5696..5714)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5724..5741)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5724..5740)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5733..5755)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5882..6337
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5969..5988)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6179..6200
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6363..6467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6468..6536,6537..7328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6686..6705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7499..8087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7988..8007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8241..8258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8375..8396
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8411..8416,8417..8434,8435..8441)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8449..8465
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8454..8476
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8473..8489
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8473..8489
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8508..8528
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8510..8528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8556..8782
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8783..8963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9010..9135
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9628..9861
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10046..10090
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10321..10531
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10343..10539
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10390..10525
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10571..11173
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10571..10590)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11067..11087
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11216..11333
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 gaggagaaaa cagcaggcac ccaagcgggc ggcaggctac gcccaggagg aacagctgaa
     1261 agaagaggag gaaataaaag aagaggagga ggaggaggac agcggttcag tagctcaact
     1321 gcagggtggc aatgacacag ggacggacga ggagctagaa acgggcccag agcaaaaagg
     1381 ctgcttcagc taccagaact ctccaggaag tcatttgtcc aatcaggatg ccgagaacga
     1441 gtctctgctg agtgacgcca gtgatcaggt gtcggacatc aagagtgtct gcggcagaga
     1501 tgcctcagac aagaaagcac acactcacgt caggcttcca aacgaagcac acaattgcat
     1561 ggataaaatg accgctgtct acgccaacat cctgtcggat tcctactggt caggcctggg
     1621 ccttggcttc aagctgtcca atagtgagag gaggaactgt gacacccgaa acggcagcaa
     1681 caagagtgat tttgattggc accaagacgc tctgtccaaa agcctgcagc agaacttgcc
     1741 ttctcggtcc gtctcgaaac ccagcctgtt cagctcggtg cagttgtacc gacagagcag
     1801 caagatgtgc gggactgtgt tcacaggggc cagcagattc cgatgccgac agtgcagcgc
     1861 ggcctatgac accctagtcg agctgactgt gcacatgaat gaaacgggcc actatcaaga
     1921 tgacaaccgc aaaaaggaca agctcagacc cacgagctat tcaaagccca ggaaaagggc
     1981 tttccaggat atggacaaag aggatgctca aaaggttctg aaatgtatgt tttgtggcga
     2041 ctcctttgat tccctccaag atttgagcgt ccacatgatt aaaacaaaac attaccaaaa
     2101 agtgcctttg aaggagccag tcccaaccat ttcctcgaaa atggtcaccc cggctaagaa
     2161 acgcgttttt gatgtcaatc ggccgtgttc ccccgattca accacaggat cttttgcaga
     2221 ttctttttct tctcagaaga acgccaactt gcagttgtcc tccaacaacc gctatggcta
     2281 ccaaaatgga gccagctaca cctggcagtt tgaggcctgc aagtcccaga tcttaaagtg
     2341 catggagtgt gggagctccc atgacacctt gcagcagctc accacccaca tgatggtcac
     2401 aggtcacttt ctcaaggtca ccagctctgc ctccaagaaa gggaagcagc tggtattaga
     2461 cccgttagca gtggagaaaa tgcagtcgtt gtctgaggcc ccaaacagtg attctctggc
     2521 tcccaagcca tccagtaact cagcatcaga ttgtacagcc tctacaactg agttaaagaa
     2581 agagagtaaa aaagaaaggc cagaggaaac cagcaaggat gagaaagtcg tgaaaagcga
     2641 ggactatgaa gatcctctac aaaaaccttt agaccctaca atcaaatatc aatacctaag
     2701 ggaggaagac ttggaagatg gctcaaaggg tggaggggac attttgaaat ctttggaaaa
     2761 tactgtcacc acagccatca acaaagccca aaacggggcc cccagctgga gtgcctaccc
     2821 cagcatccac gcagcctacc agctgtctga gggcaccaag ccgcctttgc ctatgggatc
     2881 ccaggtactg cagatccggc ctaatctcac caacaagctg aggcccattg caccaaagtg
     2941 gaaagtgatg ccactggttt ctatgcccac acacctggcc ccttacactc aagtcaagaa
     3001 agagtcagaa gacaaagatg aagcggtgaa ggagtgtggg aaagaaagtc cccacgaaga
     3061 ggcctcatct ttcagccaca gtgagggcga ttctttccgc aaaagtgaaa cacctccaga
     3121 agccaaaaag accgagctgg gtcccctgaa ggaggaggag aagctgatga aagagggcag
     3181 cgagaaggag aaaccccagc ccctggagcc cacatctgct ctgagcaatg ggtgcgccct
     3241 cgccaaccac gccccggccc tgccatgcat caacccactc agcgccctgc agtccgtcct
     3301 gaacaatcac ttgggcaaag ccacggagcc cttgcgctca ccttcctgct ccagcccaag
     3361 ttcaagcaca atttccatgt tccacaagtc gaatctcaat gtcatggaca agccggtctt
     3421 gagtcctgcc tccacaaggt cagccagcgt gtccaggcgc tacctgtttg agaacagcga
     3481 tcagcccatt gacctgacca agtccaaaag caagaaagcc gagtcctcgc aagcacaatc
     3541 ttgtatgtcc ccacctcaga agcacgctct gtctgacatc gccgacatgg tcaaagtcct
     3601 ccccaaagcc accaccccaa agccagcctc ctcctccagg gtccccccca tgaagctgga
     3661 aatggatgtc aggcgctttg aggatgtctc cagtgaagtc tcaactttgc ataaaagaaa
     3721 aggccggcag tccaactgga atcctcagca tcttctgatt ctacaagccc agtttgcctc
     3781 gagcctcttc cagacatcag agggcaaata cctgctgtct gatctgggcc cacaagagcg
     3841 tatgcaaatc tctaagttta cgggactctc aatgaccact atcagtcact ggctggccaa
     3901 cgtcaagtac cagcttagga aaacgggcgg gacaaaattt ctgaaaaaca tggacaaagg
     3961 ccaccccatc ttttattgca gtgactgtgc ctcccagttc agaacccctt ctacctacat
     4021 cagtcactta gaatctcacc tgggtttcca aatgaaggac atgacccgct tgtcagtgga
     4081 ccagcaaagc aaggtggagc aagagatctc ccgggtatcg tcggctcaga ggtctccaga
     4141 aacaatagct gccgaagagg acacagactc taaattcaag tgtaagttgt gctgtcggac
     4201 atttgtgagc aaacatgcgg taaaactcca cctaagcaaa acgcacagca agtcacccga
     4261 acaccattca cagtttgtaa cagacgtgga tgaagaatag taatgaacta gtccgtaact
     4321 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaat atcacacaga
     4381 tccacaaact gtacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     4441 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     4501 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     4561 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     4621 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     4681 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     4741 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     4801 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     4861 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     4921 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     4981 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     5041 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5101 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5161 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5221 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5281 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     5341 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     5401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     5521 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5581 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5641 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     5701 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5761 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5821 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5881 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5941 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6001 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6061 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6121 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6181 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6241 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6301 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     6481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     6541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     6601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     6661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     6721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     6781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     6841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     6901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     6961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     7321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     7381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     7441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     7501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     7561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     7621 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     7681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     7741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     7801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     7861 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     7921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     7981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     8281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     8341 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     8401 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     8461 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     8521 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     8581 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     8641 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     8701 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     8761 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     8821 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8881 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8941 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     9001 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     9061 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     9121 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     9181 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     9241 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     9301 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     9361 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     9421 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     9481 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     9541 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     9601 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     9661 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     9721 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     9781 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     9841 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     9901 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     9961 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    10021 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    10081 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    10141 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    10201 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    10261 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10321 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
    10381 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10441 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    10501 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
    10561 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
    10621 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
    10681 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
    10741 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
    10801 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
    10861 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
    10921 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
    10981 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
    11041 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
    11101 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    11161 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    11221 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    11281 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    11341 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    11401 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    11461 agtgggaatt
//
