LOCUS       sequence-38578-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Werdien D, Peiler G, Ryffel GU
  TITLE     FLP and Cre recombinase function in Xenopus embryos.
  JOURNAL   Nucleic Acids Res. 2001 Jun 1. 29(11):E53-3.
  PUBMED    11376165
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2001 Jun 1. 29(11):E53-3."
COMMENT     Sequence Label: pCMV:DsRed(FRT)GFP
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    169..303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(206..225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     260..279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    305..338
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(395..423)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcgcaccga gggccgccac cacctgttcc tgtagcggcc actcgcagca tgctctagca
       61 ggatcataat cagccatacc acatttgtag aggttttacc ttgctttaaa aaacctccca
      121 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
      181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
      241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
      301 atccgaagtt cctattctct agaaagtata ggaacttccc gggcctagga gatccacagc
      361 caccatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      421 tggtgatgtt aatgggtaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      481 cagaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      541 acttgtcact actctcactt atggtgttca atgcttttca agatatccag atcatatgaa
      601 gcggcacgac ttcttcaaga gcgccatgcc tgagggatac gtgcaggaga ggaccatctt
      661 cttcaaggac gacgggaact acaagacacg tgctgaagtc aagtttgagg gagacaccct
      721 cgtcaacagg atcgagctta agggaatcga tttcaaggag gacggaaaca tcctcggcca
      781 caagttggaa tacaactaca actcccacaa cgtatacatc atggccgaca agcaaaagaa
      841 c
//
