LOCUS       sequence-385784-        9115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9115)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2803
FEATURES             Location/Qualifiers
     source          1..9115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4105..4114
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4968..5556
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5021..5041)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5439..5450)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5628..5861
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5939..6060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5976..5995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6030..6049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6100..6235
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6255..6274)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6256..6274)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6284..6301)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6284..6300)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6293..6315)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6442..6897
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6529..6548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6739..6760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6923..7027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7028..7096,7097..7888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7246..7265)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8059..8647
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8548..8567
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8801..8818
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8935..8956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8971..8976,8977..8994,8995..9001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9009..9025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9014..9036
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9033..9049
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9033..9049
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9068..9088
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9070..9088
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atggcgtgca cagcctggga ctcttggagc
     4141 ggcgcctcgc agaccctggg ccccgcccct ctcggcccgg gccccatccc cgccgccggc
     4201 tccgaaggcg ccgcgggcca gaactgcgtc cccgtggcgg gagaggccac ctcgtggtcg
     4261 cgcgcccagg ccgccgggag caacaccagc tgggactgtt ctgtggggcc cgacggcgat
     4321 acctactggg gcagtggcct gggcggggag ccgcgcacgg actgtaccat ttcgtggggc
     4381 gggcccgcgg gcccggactg taccacctcc tggaacccgg ggctgcatgc gggtggcacc
     4441 acctctttga agcggtacca gagctcagct ctcaccgttt gctccgaacc gagcccgcag
     4501 tcggaccgtg ccagtttggc tcgatgcccc aaaactaacc accgaggtcc cattcagctg
     4561 tggcagttcc tcctggagct gctccacgac ggggcgcgta gcagctgcat ccgttggact
     4621 ggcaacagcc gcgagttcca gctgtgcgac cccaaagagg tggctcggct gtggggcgag
     4681 cgcaagagaa agccgggcat gaattacgag aagctgagcc ggggccttcg ctactactat
     4741 cgccgcgaca tcgtgcgcaa gagcgggggg cgaaagtaca cgtaccgctt cgggggccgc
     4801 gtgcccagcc tagcctatcc ggactgtgcg ggaggcggac ggggagcaga gacacaatag
     4861 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     4921 aaggacgaag cctcaagcca ttcggttagc tcacgcgtta agtcgacaat caacctctgg
     4981 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5041 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5101 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5161 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5221 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5281 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5341 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5401 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5461 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5521 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     5581 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5641 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     5701 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     5761 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     5821 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     5881 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6061 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6121 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6181 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6241 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6301 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6361 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6421 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6481 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6541 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6601 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6661 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6721 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6781 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6841 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6901 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6961 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7021 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7081 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7141 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7201 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7261 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7321 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7381 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7441 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7501 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7561 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7621 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7681 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7741 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7801 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7861 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7921 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7981 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8041 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8101 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8161 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8221 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8281 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8341 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8401 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8461 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8521 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8581 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8641 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8701 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8761 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8821 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     8881 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8941 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     9001 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     9061 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaa
//
