LOCUS       sequence-38579-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-ctH6-eGFP
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(63..69,70..87,88..93))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      191..234
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(238..256)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        324..428
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(647..666)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcgcccttgc tcaccatatg gatccagatc tcctccttct actagacgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggtctaga taagttcagg
      181 atgaattcca gaaatcatcc ttagcgaaag ctaaggattt tttttatctg aaattctgcc
      241 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      301 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      361 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      421 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      481 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      541 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      601 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      661 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      721 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      781 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      841 caacgatcgg aggaccgaag gagctaaccg cttttt
//
