LOCUS       sequence-385793-       10978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10978)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1106
FEATURES             Location/Qualifiers
     source          1..10978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6831..7419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6884..6904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7302..7313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7491..7724
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7802..7923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7839..7858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7893..7912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7963..8098
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8118..8137)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8119..8137)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8147..8164)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8147..8163)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8156..8178)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8305..8760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8392..8411)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8602..8623
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8786..8890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8891..8959,8960..9751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9109..9128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9922..10510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10411..10430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10664..10681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10798..10819
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10834..10839,10840..10857,10858..10864)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10872..10888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10877..10899
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10896..10912
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10896..10912
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10931..10951
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10933..10951
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atgacagctg acaaggagaa gaaaaggagt
     4141 agctcggaga ggaggaagga gaagtcccgg gatgctgcgc ggtgccggcg gagcaaggag
     4201 acggaggtgt tctatgagct ggcccatgag ctgcctctgc cccacagtgt gagctcccat
     4261 ctggacaagg cctccatcat gcgactggca atcagcttcc tgcgaacaca caagctcctc
     4321 tcctcagttt gctctgaaaa cgagtccgaa gccgaagctg accagcagat ggacaacttg
     4381 tacctgaaag ccttggaggg tttcattgcc gtggtgaccc aagatggcga catgatcttt
     4441 ctgtcagaaa acatcagcaa gttcatggga cttacacagg tggagctaac aggacatagt
     4501 atctttgact tcactcatcc ctgcgaccat gaggagattc gtgagaacct gagtctcaaa
     4561 aatggctctg gttttgggaa aaaaagcaaa gacatgtcca cagagcggga cttcttcatg
     4621 aggatgaagt gcacggtcac caacagaggc cgtactgtca acctcaagtc agccacctgg
     4681 aaggtcttgc actgcacggg ccaggtgaaa gtctacaaca actgccctcc tcacaatagt
     4741 ctgtgtggct acaaggagcc cctgctgtcc tgcctcatca tcatgtgtga accaatccag
     4801 cacccatccc acatggacat ccccctggat agcaagacct tcctgagccg ccacagcatg
     4861 gacatgaagt tcacctactg tgatgacaga atcacagaac tgattggtta ccaccctgag
     4921 gagctgcttg gccgctcagc ctatgaattc taccatgcgc tagactccga gaacatgacc
     4981 aagagtcacc agaacttgtg caccaagggt caggtagtaa gtggccagta ccggatgctc
     5041 gcaaagcatg ggggctacgt gtggctggag acccagggga cggtcatcta caaccctcgc
     5101 aacctgcagc cccagtgcat catgtgtgtc aactacgtcc tgagtgagat tgagaagaat
     5161 gacgtggtgt tctccatgga ccagactgaa tccctgttca agccccacct gatggccatg
     5221 aacagcatct ttgatagcag tggcaagggg gctgtgtctg agaagagtaa cttcctattc
     5281 accaagctaa aggaggagcc cgaggagctg gcccagctgg ctcccacccc aggagacgcc
     5341 atcatctctc tggatttcgg gaatcagaac ttcgaggagt cctcagccta tggcaaggcc
     5401 atcctgcccc cgagccagcc atgggccacg gagttgagga gccacagcac ccagagcgag
     5461 gctgggagcc tgcctgcctt caccgtgccc caggcagctg ccccgggcag caccaccccc
     5521 agtgccacca gcagcagcag cagctgctcc acgcccaata gccctgaaga ctattacaca
     5581 tctttggata acgacctgaa gattgaagtg attgagaagc tcttcgccat ggacacagag
     5641 gccaaggacc aatgcagtac ccagacggat ttcaatgagc tggacttgga gacactggca
     5701 ccctatatcc ccatggacgg ggaagacttc cagctaagcc ccatctgccc cgaggagcgg
     5761 ctcttggcgg agaacccaca gtccaccccc cagcactgct tcagtgccat gacaaacatc
     5821 ttccagccac tggcccctgt agccccgcac agtcccttcc tcctggacaa gtttcagcag
     5881 cagctggaga gcaagaagac agagcccgag caccggccca tgtcctccat cttctttgat
     5941 gccggaagca aagcatccct gccaccgtgc tgtggccagg ccagcacccc tctctcttcc
     6001 atggggggca gatccaatac ccagtggccc ccagatccac cattacattt tgggcccaca
     6061 aagtgggccg tcggggatca gcgcacagag ttcttgggag cagcgccgtt ggggccccct
     6121 gtctctccac cccatgtctc caccttcaag acaaggtctg caaagggttt tggggctcga
     6181 ggcccagacg tgctgagtcc ggccatggta gccctctcca acaagctgaa gctgaagcga
     6241 cagctggagt atgaagagca agccttccag gacctgagcg ggggggaccc acctggtggc
     6301 agcacctcac atttgatgtg gaaacggatg aagaacctca ggggtgggag ctgccctttg
     6361 atgccggaca agccactgag cgcaaatgta cccaatgata agttcaccca aaaccccatg
     6421 aggggcctgg gccatcccct gagacatctg ccgctgccac agcctccatc tgccatcagt
     6481 cccggggaga acagcaagag caggttcccc ccacagtgct acgccaccca gtaccaggac
     6541 tacagcctgt cgtcagccca caaggtgtca ggcatggcaa gccggctgct cgggccctca
     6601 tttgagtcct acctgctgcc cgaactgacc agatatgact gtgaggtgaa cgtgcccgtg
     6661 ctgggaagct ccacgctcct gcaaggaggg gacctcctca gagccctgga ccaggccacc
     6721 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     6781 ggaaaggacg aattatcgtg gatcaagttt ccatcacgcg ttaagtcgac aatcaacctc
     6841 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     6901 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6961 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     7021 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     7081 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     7141 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     7201 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     7261 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     7321 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     7381 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     7441 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     7501 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7561 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7621 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7681 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     7741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8041 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8101 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8161 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8221 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8281 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8341 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8401 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8461 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8521 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8581 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8641 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8701 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10561 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
    10621 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
    10681 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
    10741 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
    10801 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
    10861 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
    10921 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaa
//
