LOCUS       sequence-385807-        9043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9043)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1849
FEATURES             Location/Qualifiers
     source          1..9043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1766..1976
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1788..1984
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1835..1970
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2016..2618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2016..2035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2512..2532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2661..2778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2916..4094
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3147..4085
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4042..4062
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4896..5484
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4949..4969)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5367..5378)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5556..5789
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5867..5988
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5904..5923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5958..5977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6028..6163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6183..6202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6184..6202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6212..6229)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6212..6228)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6221..6243)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6370..6825
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6457..6476)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6667..6688
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6851..6955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6956..7024,7025..7816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7174..7193)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7987..8575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8476..8495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8729..8746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8863..8884
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8899..8904,8905..8922,8923..8929)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8937..8953
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8942..8964
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8961..8977
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8961..8977
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8996..9016
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8998..9016
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag
     1741 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta
     1801 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1861 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1921 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1981 caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc
     2041 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     2101 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     2161 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     2221 acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     2281 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     2341 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     2401 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     2461 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     2521 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     2581 gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa
     2641 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2701 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2761 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg
     2821 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
     2881 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
     2941 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
     3001 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     3061 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     3121 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
     3181 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
     3241 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
     3301 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
     3361 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
     3421 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
     3481 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
     3541 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
     3601 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
     3661 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
     3721 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
     3781 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     3841 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     3901 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     3961 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     4021 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     4081 ttcaggtgtc gtgaggctag caccgccacc atgaaggcgg ccgtcgatct caagccgact
     4141 ctcaccatca tcaagacgga aaaagtcgat ctggagcttt tcccctcccc gggtaaactc
     4201 gggggccagg actcttttga aagcatagag agctacgata gttgtgatcg cctcacccag
     4261 tcctggagca gccagtcatc tttcaacagc ctgcagcgtg ttccctccta tgacagcttc
     4321 gactcagagg actatccggc tgccctgccc aaccacaagc ccaagggcac cttcaaggac
     4381 tatgtgcggg accgtgctga cctcaataag gacaagcctg tcattcctgc tgctgcccta
     4441 gctggctaca caggcagtgg accaatccag ctatggcagt ttcttctgga attactcact
     4501 gataaatcct gtcagtcttt tatcagctgg acaggagatg gctgggaatt caaactttct
     4561 gacccagatg aggtggccag gagatgggga aagaggaaaa acaaacctaa gatgaattat
     4621 gagaaactga gccgtggcct acgctactat tacgacaaaa acatcatcca caagacagcg
     4681 gggaaacgct acgtgtaccg ctttgtgtgt gacctgcaga gcctgctggg gtacacccct
     4741 gaggagctgc acgccatgct ggacgtcaag ccagatgccg acgagtagta atgaactagt
     4801 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatgaa
     4861 ggcgatttaa ctgactctga acgcgttaag tcgacaatca acctctggat tacaaaattt
     4921 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4981 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5041 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5101 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5161 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5221 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5281 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5341 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5401 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5461 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     5521 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5581 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5641 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5701 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5761 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     5821 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6001 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6061 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6121 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6181 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6241 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6301 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6361 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6421 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6481 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6541 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6601 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6661 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6721 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6781 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6841 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6901 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6961 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7021 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7081 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7141 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7201 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7261 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7321 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7381 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7441 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7501 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7561 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7621 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7681 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7741 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7801 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7861 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7921 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7981 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8041 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8101 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8161 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8221 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8281 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8341 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8401 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8461 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8521 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8581 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8641 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8701 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8761 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8821 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8881 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8941 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9001 taaccctcac taaagggaac aaaagctgga gctgcaagct taa
//
