LOCUS       sequence-38581-e         520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Roose M, Sauert K, Turan G, Solomentsew N, Werdien D, Pramanik K, 
            Senkel S, Ryffel GU, Waldner C
  TITLE     Heat-shock inducible Cre strains to study organogenesis in 
            transgenic Xenopus laevis.
  JOURNAL   Transgenic Res. 2009 Aug . 18(4):595-605.
  PUBMED    19266305
REFERENCE   2  (bases 1 to 520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res. 2009 Aug . 18(4):595-605."
COMMENT     Sequence Label: pBSHSP:Cre;CMV:tdTomato-SceI
FEATURES             Location/Qualifiers
     source          1..520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        53..71
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     53..70
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(243..261)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gcatgctcgt tagggaaccg ccattctgcc tggggacgtc ggagcaagct tgatttaggt
       61 gacactatag aatacaagct acttgttctt tttgcaggat cccgccacca tggtgagcaa
      121 gggcgaggag gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
      181 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      241 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      301 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
      361 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      421 tctggtgacc gtgacccang actcctccct gcaggacggc acgctgatct acaaggtgaa
      481 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg
//
