LOCUS       sequence-385811-       10355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GluRIIA-Rescue-Construct  for integration at attP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10355)
  AUTHORS   Beckers CJ, Mrestani A, Komma F, Dannhauser S
  TITLE     Versatile Endogenous Editing of GluRIIA in Drosophila melanogaster.
  JOURNAL   Cells. 2024 Feb 10;13(4):323. doi: 10.3390/cells13040323.
  PUBMED    38391936
REFERENCE   2  (bases 1 to 10355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cells."; 
            date: "2024-02-10"; volume: "13(4):323. doi"; pages: " 
            10.3390/cells13040323"
COMMENT     Sequence Label: pCB15
FEATURES             Location/Qualifiers
     source          1..10355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(20..50,51..252)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    372..405
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    4665..4698
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(7747..8301,8302..8332)
                     /label=P element 5' end
     rep_origin      complement(8567..9155)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8647..8666)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9326..10117,10118..10186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9949..9968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10187..10291)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgatgagaa tggccagacc atgatgaaat aacataaggt ggtcccgtcg gcaagagaca
       61 tccacttaac gtatgcttgc aataagtgcg agtgaaagga atagtattct gagtgtcgta
      121 ttgagtctga gtgagacagc gatatgattg ttgattaacc cttagcatgt ccgtggggtt
      181 tgaattaact cataatatta attagacgaa attattttta aagttttatt tttaataatt
      241 tgcgagtacg caaagctaga gttgttggtt ggcacaccac aaatatactg ttgccgagca
      301 caattccgct cgaattgccg gctgtacact taagaagttc ctactgcagg taatgaattg
      361 ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacgaattca
      421 ctggagagag cttcccatcg gctgagatga cattcttggc cgtgccagtg agttccggca
      481 gtcagtaggc gtgcgaagcg ctccaagact gggtgcttcc gttgcgtgaa aataaattgt
      541 gaagaagaaa gacggcactt tgtatctgct cgttttgaat tttttttttc tgtggcagtc
      601 tgtgtgcgtc tgtagatata gatttgtaca tatgcagttg ggaagtgaac acgaaatggc
      661 tgctgcattt agaaaattca attagtttcg agtgtgttga ggtaaacgaa aaaatgcgcc
      721 tttgtccagt tgtcatctat gcatttataa tcatcatcgg gtttctggag gggattatag
      781 cccttggtgg cgacgatcgc aatgaaatca cagtgggtag gtccctccat taattactta
      841 gtgatagcaa tagctcaacg ttttgccctc gaaggtgcca ttttctacga gaacgagaag
      901 gagatcgagc tcagcttcga tcaggccttt cgcgaggtca acaatatgaa gttctcggag
      961 ctgcgattcg tgaccatcaa acgctatatg cccaccaacg acagtttcct tttgcagcag
     1021 attagtaagt aaatacgctc ggatgcggca gaaccaagtt acgagaaatt catcttccgt
     1081 gtgtagcatg tgaactgatt tccaatggcg ttgcggctat tttcggacca agttcaaagg
     1141 cagccagtgg taagaaaagc acacacacac acacacactg tgtgtggaca tggcccaaaa
     1201 catatgttcg cgatgtgacg gttcccccct ttcgtggcag atattgttgc gcaaatcgcg
     1261 aacgcgaccg gcattccaca cattgagtac gatttgaagc tggaggccac gcgtcaggag
     1321 cagctcaacc accagatgtc catcaatgtg gcaccctcct tgtcagtact ctcgcgtgcc
     1381 tacttcgaga tcatcaaatc caactatgag tggcgcacct tcactctgat ctatgagact
     1441 ccggagggta agtagtgcct ggataccccg tagagatatg gactacattt gaagtcctta
     1501 ggcctggcgc gcctgcagga tctgatgaac atccaagcgc tgaacagtga ctatgtcaag
     1561 ctgcgaaatt tggcagacta tgcggatgac tatcggatat tgtggaagga gacagacgag
     1621 actttccacg agcagcgcat cattttggac tgtgagccga agacccttaa ggagctgctt
     1681 aaagtatcca tagactttaa gttgcagggc ccgtttaggg taattcttca tttatgaaag
     1741 cgagaaagta cttactaagt cgctttatct ttagaactgg tttctcaccc atctggatac
     1801 gcataactcg ggtctgaggg atatttataa tgaggacttc aaggccaaca ttacgtcggt
     1861 gcgattgaaa gtggtcgacg cgaatccatt cgagagaaag aagacccggc tcaccaaagt
     1921 ggatgtaagt ggaggcccaa ctgactgtaa ccttggatct aatctagcgt acttcgcagc
     1981 aaatattggg gaaccaaacc atgttgccca ttctcatcta cgacgctgtc gtcctgtttg
     2041 ccagttctgc ccgaaatgtt attgctgcca tgcagccctt tcatccaccc aatcgccatt
     2101 gcggcagttc gtctccttgg atgctgggag catttatagt caacgagatg aagaccattt
     2161 ccgaggatga tgtggaaccg cactttaaga ccgaaaatat gaagcttgac gagtatgggc
     2221 agcgcatcca cttcaacctg gagatctaca agccaacggt gaacgaacca atgatggtct
     2281 ggacaccaga caatgggata aagaagcgtc tcctcaactt ggaactggaa agtgccggca
     2341 ccacccagga tttctcggag cagcgtaaag tgtatacagt ggttacccac tacgaggagc
     2401 cctacttcat gatgaagttg gttttgaagc agtaatctgg tttttagcag actacaacct
     2461 ttccttattt cctttaggga ggatcacgag aacttcagag gcagggaaaa gtacgagggc
     2521 tatgccgtgg atctcattag caagctttcc gagctcatgg agttcgatta cgagttcatg
     2581 atcgttaatg gcaatggcaa atacaatccg gagacgaagc aatgggatgg cattatacgg
     2641 aaactgatag accacgtagg tttcaccaga agtatagtag taactccact tgatatttgt
     2701 atcttttata cacagcatgc ccaaatcggc gtctgcgatc tgaccatcac ccaaatgcga
     2761 cgttctgtgg tggacttcac ggtgcccttc atgcagttgg gcattagcat tctgcactac
     2821 aagagtcctc cggagccgaa gaatcagttt gccttcttgg aaccgtttgc cgtcgaagtc
     2881 tggatctata tgatctttgc ccagttgata atgactttgg cttttgtttt catagccagg
     2941 tgggattagg ttaacgctct tgagtgaagc ttaatttact tttcttacag actttcgtat
     3001 cgcgaatggt tgccacctaa tcccgccatt caggatccag atgagctgga aaatatctgg
     3061 aatgtgaaca actcgacttg gctgatggtg ggctccatca tgcaacaggg ctgcgacatt
     3121 ctgcctaggt ctcagattat atattacaca gtatcaatgg gtatcgaaat gtaaactttc
     3181 ttcactcaca gaggtccaca catgcgcata ctcactggca tgtggtggtt cttcgccctg
     3241 atgatgctca gtacctacac ggccaacttg gccgccttcc tgaccagcaa caagtggcag
     3301 agcagcataa agagcctcca ggacctcatc gaacaggata aggtgcactt cggcagcatg
     3361 cgcggtggca gcacctcgtt gttcttttcg gagtcgaatg acacggatta ccagcgtgcg
     3421 tggaaccaga tgaaggactt caatccctcg gccttcacca gcaccaacaa agagggagtg
     3481 gcacgtgtgc gcaaggagaa gggcggctat gcctttctga tggagaccac cagtttgacc
     3541 tacaatatcg aacggaactg cgatctcacc cagattggcg agcagatcgg ggagaagcac
     3601 tacggcctgg ccgtgccact gggtgagtag ctgtgccgcc agtggaggga gttgggatat
     3661 tactttcttc catttcaacc aggctctgat taccggacca acctcagcgt gtccattctc
     3721 cagctgagcg aaaggggcga gctgcagaag atgaagaaca agtggtggaa gaaccacaat
     3781 gtcacctgtg actcctacca cgaggtggat ggtgacgagc tgtccattat tgagctgggc
     3841 ggcgtgtttc ttgttctggc cggtggcgtt ctcatcggcg ttatcctggg catctttgag
     3901 ttcctgtgga acgtgcagaa cgtggcggtg gaggagcgag tgactccgtg gcaggctttc
     3961 aaggcggagc tcatctttgc cctgaagttt tgggtgcgca aaaagccgat gcgcatctcc
     4021 agcagcagcg acaagtcgtc gtccaggcga tcatccggtt ccagacgcag ctccaaggaa
     4081 aagtcccgca gcaagacggt tagctaggtg gtcggaatat tggacgattg gcgtcttcta
     4141 ctttggcatt tcattagaga gtcgcattgg aaatgaggca ctccatggtg tccacattgt
     4201 atattgtgag ctatatttgt agaattattt tagaacacag atagcgcatt tgggtatatt
     4261 gaatgttctg aagatttaac gaaactttca atgaaataat ttggataact aattttagta
     4321 aacttttctg ttggctctaa tgttttaata gttgacaata attgattttc cttacaaaga
     4381 tttaataaat aaacattaaa atataatatg tgtgtttata tacaatatat agtcagttag
     4441 ttgtgaaatt atattctata catttctttt gaaagctgcc taattttaga ccacatttag
     4501 ttttcattca ctggtccatt tccagctaat gctcgtgtct gcatggtcta gattaaagag
     4561 tatagagtat agacgctaga ctctaaagtc tagacatgtg gtgattgctc ttctcgccga
     4621 gcatggaatc catccacaat ggctgctgtt ctccatgagg taccataact tcgtataatg
     4681 tatgctatac gaagttatcg tacgactagc acctgactgt cgagcccagt ggaaaccctt
     4741 gaaatgcctt taagtcgagc ccagtggaaa cccttgaaat gcctttaagt cgagcccagt
     4801 ggaaaccctt gaaatgcctt taagtcgagc ccagtggaaa cccttgaaat gcctttaagt
     4861 cgagcccagt ggaaaccctt gaaatgcctt taagtcgagt agcagtcgag gtaggtacgc
     4921 catgccccgt tattctctat tcgttttgtg actctccctc tctgtactat tgctctctca
     4981 ctctgtcgca cagtaaacgg cacgctattc tcgttgcttc gagagagcgc gcctcgaatg
     5041 ttcgcgaaaa gagcgccgga gtataaatag aggcgcttcg tcgacggagc gtcaattcaa
     5101 ttcaaacaag caaagtgaac acatcgcgaa gcgtaagctg agcaaacaaa caagcgcagc
     5161 tgaacaagct aaacaatctg caataaagtg caagttaaag tgaatcaatt aaaagtaacc
     5221 aacaaccaag taattaaact aaaaactgca actactgaaa tcaaccaaga agtcattatt
     5281 gaagacaaga agagaactct gaataggtcg atagcgtcaa ttcggcacga gcggtttcgt
     5341 gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc caaaactcct
     5401 ctcgcttctt atttttgttt gttttttgag tgattgggtg ggtgattggt tttgggtggg
     5461 taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg agctattaat
     5521 tcgcggaggc agcaaacacc catctgccga gcatctgaac aatggtgaca gcggagcggc
     5581 ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa aactacggca cgctcctgcc
     5641 acccagtccg ccggaggact ccggttcagg gagcggccaa ctagccgaga acctcaccta
     5701 tgcctggcac aatatggaca tctttggggc ggtcaatcag ccgggctccg gatggcggca
     5761 gctggtcaac cggacacgcg gactattctg caacgagcga cacataccgg cgcccaggaa
     5821 acatttgctc aagaacgttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg
     5881 cagttccggt gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg
     5941 catccaagta tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga
     6001 gatgcaggcc aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc
     6061 cagggaacac ctgattttcc aggccatggt gcggatgcca cgacatctga cctatcggca
     6121 gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac
     6181 gatcatcggt gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc
     6241 attcgcctcc gaggcactaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg
     6301 actggactca tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg
     6361 caagaccgtc atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa
     6421 gatccttctg atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga
     6481 cttcttttcc tacgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     6541 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
     6601 atgcgacaat tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa
     6661 aaatttggag aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt
     6721 catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct
     6781 cgtaaaagtg cgacttattc agacaacgat ggttgccatc ttgattggcc tcatcttttt
     6841 gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac ggagccatct tcctcttcct
     6901 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtgttcacct cagagctgcc
     6961 agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact ttctgggcaa
     7021 aacgattgcc gaattgccgc tttttctcac agtgccactg gtcttcacgg cgattgccta
     7081 tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg cgctggtcac
     7141 tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca gctcctcgac
     7201 ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct ttggcggctt
     7261 cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt
     7321 ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat
     7381 tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct
     7441 taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc tcatcgtgag
     7501 cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg agtagccgac
     7561 atatatccga aataactgtt tgtttttttt ttttaccatt attaccatcg tgtttactgt
     7621 ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac aagatatgac
     7681 ctatagaata caaaaaaaaa aaaaaaaaaa aaaaaaaaaa ctcgacaccg ggatccaaac
     7741 ctagtggctg ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca
     7801 agctcttatt ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca
     7861 taaatgattt tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct
     7921 tcttttatct tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt
     7981 cgcctccttt taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag
     8041 tgggtatcac aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat
     8101 tttctcttaa tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag
     8161 caaaatttgc aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa
     8221 aattcgtccg cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat
     8281 acttcggtaa gcttcggcta tcgacgggac caccttatgt tatttcatca tgggccagac
     8341 ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg atgaccttgc
     8401 ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc tccacgtccg
     8461 cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg tacgacaacc
     8521 atttgaggta tactggcacc gagcccgagt tcaagaagaa ggcgtttttc cataggctcc
     8581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     9001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     9061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     9121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctcgaa
    10321 gtcttagagc cagatatgcg agcacccgga agctc
//
