LOCUS       sequence-385845-        3450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BARF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BARF1
FEATURES             Location/Qualifiers
     source          1..3450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1329..1428)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1445..1464)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1446..1464)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1469..1485)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1469..1485)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1916..2707
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2800..3388
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3289..3308
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gccaggttca tcgctcagct cctcctgttg gcctcctgtg tggccgccgg
      721 ccaggctgtc accgctttct tgggtgagcg agtcaccctg acctcctact ggaggagggt
      781 gagcctcggt ccagagattg aggtcagctg gtttaaactg ggcccaggag aggagcaggt
      841 gcttattggg cgcatgcacc acgatgtcat ctttatagag tggcctttca ggggcttctt
      901 tgatatccac agaagtgcca acaccttctt tttagtagtc accgctgcca acatctccca
      961 tgacggcaac tacctgtgcc gcatgaaact gggcgagacc gaggtcacca agcaggaaca
     1021 cctgagcgtg gtgaagcctc taacgctgtc tgtccactcc gaaaggtctc agttcccaga
     1081 cttctctgtc cttactgtga catgcaccgt gaatgcattt ccccatcccc acgtccagtg
     1141 gctcatgccc gagggcgtgg agcccgcacc aactgcggca aatggcggtg ttatgaagga
     1201 aaaggatggg agcctctctg ttgctgttga cctgtcactt cccaagccct ggcacctgcc
     1261 agtgacctgc gttgggaaaa atgacaagga ggaagcccac ggggtttatg tttctggata
     1321 cttgtcgcaa ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1381 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     1441 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     1501 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     1561 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     1621 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     1681 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     1741 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     1801 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     1861 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     1921 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     1981 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     2041 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     2101 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     2161 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     2221 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     2281 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     2341 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     2401 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     2461 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     2521 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     2581 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     2641 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     2701 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     2761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2941 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3181 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     3361 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     3421 ggccttttgc tggccttttg ctcacatgtt
//
