LOCUS       sequence-385846-        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is used to express a EF-hand disabled human MICU1 
            protein in E. coli. The protein is fused with a maltose binding 
            protein in the N-terminus, and also contains a C-terminal His6 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Tsai CW, Liu TY, Chao FY, Tu YC, Rodriguez MX, Van Keuren AM, Ma Z, 
            Bankston J, Tsai MF
  TITLE     Evidence supporting the MICU1 occlusion mechanism and against the 
            potentiation model in the mitochondrial calcium uniporter complex.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Apr 18;120(16):e2217665120. doi: 
            10.1073/pnas.2217665120. Epub 2023 Apr 10.
  PUBMED    37036971
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-04-18"; volume: "120(16):e2217665120. 
            doi"; pages: " 10.1073/pnas.2217665120. Epub 2023 Apr 10"
COMMENT     Sequence Label: pET-hMICU1EF
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     112..135
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             139..156
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             157..177
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1471..1488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaaaacg cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg
       61 tatgccgtgc gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc
      121 ctgaaagacg cgcagactct ggttccgcgt ggatcggaaa acctgtattt tcagggcgga
      181 tccaagaggg cccatgcaga atctccacca tgtgtagaca acctaaaaag tgacatcggt
      241 gataaaggga agaataaaga tgaaggggat gtttgtaacc atgagaaaaa gactgcagat
      301 cttgcccctc acccagaaga gaaaaagaag aaacgttctg gattcagaga cagaaaagtg
      361 atggaatatg agaataggat tcgagcctac tccacgccag acaaaatctt ccgatatttt
      421 gccaccttga aagtcatcag tgagcctggt gaagcagaag tgtttatgac accagaagat
      481 tttgtgcgat ccataacacc caatgaaaaa caaccagaac acttgggtct ggatcaatat
      541 ataataaaac gctttgatgg aaagaaaatt tcccaggaac gagaaaaatt tgctgatgaa
      601 ggcagtatat tttacaccct tggagaatgt gggctcatat ccttttcaga ctacattttc
      661 ctcacaactg ttctttccac tcctcagaga aattttgaaa ttgccttcaa gatgtttgct
      721 ttgaatggag atggagaagt agatatggaa aaatttgaac aggttcagag catcattcgc
      781 tcccaaacca gtatgggtat gcgccacaga gatcgtccaa ctactggcaa caccctcaag
      841 tctggcttgt gttcagccct cacaacctac ttttttggag ctgatctgaa gggaaagctg
      901 acaatcaaaa acttcctcga atttcagcgt aaactgcagc atgatgttct gaagcttgag
      961 tttgaacgcc atgaccctgt ggatgggaga attactgaga ggcaatttgg tggcatgcta
     1021 cttgcctaca gtggggtgca gtccaagaag ctgaccgcca tgcagaggca gctcaagaag
     1081 cacttcaaag aaggaaaggg tctgacattt caggaggtgg agaacttctt tactttccta
     1141 aagaacatta atgatgtgga cactgcattg agtttttacc acatggctgg agcatctctt
     1201 gataaagtga ccatgcagca ggtggccagg acagtggcta aagtggagct ctcagaccac
     1261 gtgtgtgatg tggtgtttgc actctttgcc tgtgatggca atggcgaact gagcaataag
     1321 aaatgggttt ccatcatgaa gcaacggctg atgagaggcc tggaaaagcc caaagacatg
     1381 ggttggactc gcctcatgca ggccatgtgg aaatgtgcac aggaaactgc ctgggacttc
     1441 gctttaccca aacaggcggc cgcactcgag caccaccacc accaccactg a
//
