LOCUS       sequence-38586-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pACBB-eGFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(63..69,70..87,88..93))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     764..783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcgcccttgc tcaccatatg gatccagatc tcctccttct actagacgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggtctaga taagttcagg
      181 atgaattcat cgatgataag ctgtcaaaca tgagaattac aacttatatc gtatggggct
      241 gacttcaggt gctacatttg aagagataaa ttgcactgaa atcttgaaat attttatctg
      301 attaataaga tgatcttctt gagatcgttt tggtctgcgc gtaatctctt gctctgaaaa
      361 cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc tgagctacca actctttgaa
      421 ccgaggtaac tggcttggag gagcgcagtc accaaaactt gtcctttcag tttagcctta
      481 accggcgcat gacttcaaga ctaactcctc taaatcaatt accagtggct gctgccagtg
      541 gtgcttttgc atgtctttcc gggttggact caagacgata gttaccggat aaggcgcagc
      601 ggtcggactg aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg
      661 aactgagtgt caggcgtgga atgagacaaa cgcggccata acagcggaat gacaccggta
      721 aaccgaaagg caggaacagg agagcgcacg agggagccgc caggggaaac gcctggtatc
      781 tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttg
//
