LOCUS       sequence-385863-       10165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit 
            (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10165)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Gai2
FEATURES             Location/Qualifiers
     source          1..10165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3010..3027)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3039..3612
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3062..3614
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3206..3223)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3433..3452
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5756..5773)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5762..5986
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6032..6460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6119..6138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6329..6350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6469..6489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6474..6803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6654..6789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6716..6735
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6870..7664
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6924..6943)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7534..7553
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7838..7959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7875..7894)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7929..7948
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8008..8024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8008..8024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8021..8043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8032..8048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8056..8062,8063..8080,8081..8086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8101..8122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8239..8256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8410..8998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8490..8509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9169..9960,9961..10029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9792..9811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10030..10134)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtctaaggg agaggagctg ttcaccggag tggtgcctat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gagctacggc gtgcagtgtt tttcccggta
     2221 tcccgaccac atgaagcagc acgatttctt taagagcgcc atgcctgagg gctacgtgca
     2281 ggagaggaca atcttcttta aggacgatgg caactataag acccgcgccg aggtgaagtt
     2341 cgagggcgac acactggtga accggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt acatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 ctccgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gaccagtgct
     2581 gctgcccgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggaatcacac tgggaatgga
     2701 cgagctgtat aagggctccg ccggcaccat gtctaacaat atggccaaga tcgccgaggc
     2761 cagaaagaca gtggagcagc tgaagctgga ggtcaatatc gataggatga aggtgtctca
     2821 ggctgccgcc gagctgctgg ccttttgcga gacccacgcc aaggacgatc ctctggtgac
     2881 acccgtgcct gcagcagaga acccattccg ggataagcgg ctgttctgcg tgctgctgtg
     2941 accgcggcgt acgaggcctg catgctccgg acctgcaggt tcgaagtcga cagatctcaa
     3001 ttggggcccc tatagtgtca cctaaataat tccgcccccc cctctccctc ccccccccct
     3061 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3121 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3181 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3241 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3301 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3361 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3421 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3481 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3541 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     3601 acacgatgat aatatgggct gcaccgtgag cgcagaggat aaggctgccg ccgagaggtc
     3661 caagatgatc gataagaacc tgagggagga cggagagaag gcagcccgcg aggtgaagct
     3721 gctgctgctg ggagcaggag agtctggcaa gagcaccatc gtgaagcaga tgaagatcat
     3781 ccacgaggat ggctattccg aggaggagtg taggcagtac cgcgccgtgg tgtattccaa
     3841 cacaatccag tctatcatgg ccatcgtgaa ggcaatggga aattctggag gaggaggctc
     3901 catggcatct aaggtgtacg acccagagca gcggaagaga atgatcaccg gaccacagtg
     3961 gtgggcaaga tgcaagcaga tgaacgtgct ggatagcttt atcaattact atgactccga
     4021 gaagcacgcc gagaacgccg tgatcttcct gcacggcaat gccacaagct cctatctgtg
     4081 gaggcacgtg gtgccccaca tcgagcctgt ggccagatgt atcatccctg atctgatcgg
     4141 catgggcaag agcggcaagt ccggaaacgg cagctacagg ctgctggacc actacaagta
     4201 tctgaccgcc tggttcgagc tgctgaatct gccaaagaag atcatctttg tgggacacga
     4261 ctggggagca gcactggcat tccactacgc ctatgagcac caggatagaa tcaaggccat
     4321 cgtgcacatg gagtctgtgg tggacgtgat cgagagctgg gacgagtggc ccgacatcga
     4381 ggaggacatc gccctgatca agagcgagga gggcgagaag atggtgctgg agaacaattt
     4441 ctttgtggag accgtgctgc catccaagat catgaggaag ctggagccag aggagttcgc
     4501 agcatacctg gagcctttta aggagaaggg cgaggtgcgg agacctacac tgagctggcc
     4561 acgcgagatc ccactggtga agggaggcaa gccagatgtg gtgcagatcg tgcggaacta
     4621 caatgcctat ctgagagcct ccgacgatct gcccaagctg ttcatcgagt ctgaccctgg
     4681 cttctttagc aacgccatcg tggagggcgc caagaagttc cccaataccg agtttgtgaa
     4741 ggtgaagggc ctgcactttc tgcaggagga cgcccctgat gagatgggca agtacatcaa
     4801 gtccttcgtg gagcgggtgc tgaagaacga gcagtccgga ggaggaggct ccctgcagat
     4861 cgactttgcc gatccatcta gggccgacga tgcaaggcag ctgttcgccc tgagctgcac
     4921 agcagaggag cagggagtgc tgccagacga tctgagcggc gtgatcagga ggctgtgggc
     4981 agaccacgga gtgcaggcct gtttcggaag gtcccgcgag tatcagctga acgattctgc
     5041 cgcctactat ctgaatgacc tggagagaat cgcccagtcc gattacatcc ctacccagca
     5101 ggacgtgctg cggacaagag tgaagaccac aggcatcgtg gagacccact tcaccttcaa
     5161 ggatctgcac ttcaagatgt ttgacgtggg cggccagcgg tctgagagaa agaagtggat
     5221 tcactgcttc gagggcgtga ccgccatcat cttttgtgtg gccctgagcg cctatgatct
     5281 ggtgctggcc gaggacgagg agatgaacag gatgcacgag tccatgaagc tgttcgattc
     5341 tatctgcaac aataagtggt ttaccgacac atccatcatc ctgttcctga ataagaagga
     5401 tctgtttgag gagaagatca cccactctcc tctgacaatc tgttttccag agtacacagg
     5461 cgccaacaag tatgacgagg ccgcctctta catccagagc aagtttgagg atctgaataa
     5521 gcgcaaggac accaaggaga tctacaccca cttcacatgc gccaccgata caaagaatgt
     5581 gcagttcgtg tttgatgccg tgacagacgt gatcatcaag aacaatctga aggactgtgg
     5641 cctgttctga ggtaccgagc tcggatccac tagtccagtg tggtggaatt ctgcagatat
     5701 ccagcacagt ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     5761 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5821 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5881 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5941 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6001 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     6061 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6121 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6181 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6241 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6301 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6361 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6421 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     6481 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6541 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6601 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6661 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6721 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6781 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6841 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6901 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6961 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     7021 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     7081 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     7141 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     7201 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     7261 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     7321 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     7381 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     7441 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     7501 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     7561 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     7621 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     7681 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     7741 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7801 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7861 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7921 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7981 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8041 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8161 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     8221 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8281 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8341 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8401 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8461 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8521 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8581 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8641 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8701 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8761 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8821 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8881 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8941 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9001 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9061 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9121 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9181 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9241 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9301 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9361 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9421 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9481 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9541 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9601 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9661 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9721 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9781 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9841 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9901 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9961 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10021 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10081 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10141 tccccgaaaa gtgccacctg acgtc
//
