LOCUS       sequence-385870-       10213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit 
            (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Ga15
FEATURES             Location/Qualifiers
     source          1..10213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3001..3018)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3030..3603
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3053..3605
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3197..3214)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3424..3443
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5804..5821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5810..6034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6080..6508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6167..6186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6377..6398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6517..6537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6522..6851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6702..6837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6764..6783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6918..7712
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6972..6991)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7582..7601
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7886..8007
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7923..7942)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7977..7996
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8056..8072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8056..8072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8069..8091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8080..8096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8104..8110,8111..8128,8129..8134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8149..8170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8287..8304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8458..9046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8538..8557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9217..10008,10009..10077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9840..9859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10078..10182)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcccat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
     2221 tcccgaccac atgaagcagc acgatttctt taagtctgcc atgcctgagg gctacgtgca
     2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
     2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatcct cacaacgtgt atatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 ctctgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gaccagtgct
     2581 gctgcccgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggaatcacac tgggaatgga
     2701 cgagctgtac aagggcagcg ccggcaccat ggaggagtgg gatgtgcctc agatgaagaa
     2761 ggaggtggag agcctgaagt atcagctggc ctttcagcgg gagatggcct ccaagacaat
     2821 cccagagctg ctgaagtgga tcgaggacgg catccccaag gaccccttcc tgaatcctga
     2881 tctgatgaag aacaatccat gggtggagaa gggcaagtgc accatcctgt gaccgcggcg
     2941 tacgaggcct gcatgctccg gacctgcagg ttcgaagtcg acagatctca attggggccc
     3001 ctatagtgtc acctaaataa ttccgccccc ccctctccct cccccccccc taacgttact
     3061 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     3121 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     3181 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     3241 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     3301 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     3361 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     3421 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     3481 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     3541 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     3601 taatatggcc agaagcctga cctggaggtg ctgtccctgg tgcctgacag aggatgagaa
     3661 ggccgccgcc cgggtggacc aggagatcaa cagaatcctg ctggagcaga agaagcagga
     3721 taggggagag ctgaagctgc tgctgctggg accaggagag agcggcaagt ccacctttat
     3781 caagcagatg cggatcatcc acggagcagg atactctgag gaggagagga agggcttccg
     3841 gccactggtg tatcagaata tctttgtgag catgcgcgcc atgatcgagg ccatggagag
     3901 actgcagatc ccctttagca ggcctgagtc caagcaccac gccagcctgg tcatgagcca
     3961 ggacccttac aaggtgacca cattcgagaa gcggtatgct gccgccatgc agtggctgtg
     4021 gagggatgca ggcatccggg catgctacga gaggagaagg gagttccacc tgctggactc
     4081 tgccgtgtac tatctgagcc acctggagag aatcaccgag gagggatacg tgccaacagc
     4141 acaggatgtg ctgcgcagcc ggatgcctac cacaggcatc aacgagtatt gtttttccgt
     4201 gcagaagaca aatctgcgca tcgtggacgt gggaggacag aagtccgagc ggaagaagtg
     4261 gattcactgc ttcgagaacg tgatcgccct gatctacctg gcctctctga gcgagtatga
     4321 tcagtgtctg gaggagtctg gaggaggagg ctctatggca agcaaggtgt acgaccctga
     4381 gcagagaaag aggatgatca ccggaccaca gtggtgggca aggtgcaagc agatgaacgt
     4441 gctggatagc tttatcaatt actatgactc cgagaagcac gccgagaacg ccgtgatctt
     4501 cctgcacggc aatgccacaa gctcctacct gtggagacac gtggtgccac acatcgagcc
     4561 cgtggccagg tgtatcatcc ctgatctgat cggcatgggc aagtccggca agtctggcaa
     4621 cggctcctat aggctgctgg accactacaa gtatctgacc gcctggttcg agctgctgaa
     4681 tctgccaaag aagatcatct ttgtgggaca cgactgggga gcagcactgg cattccacta
     4741 cgcctatgag caccaggatc gcatcaaggc catcgtgcac atggagtctg tggtggacgt
     4801 gatcgagagc tgggacgagt ggcccgacat cgaggaggac atcgccctga tcaagtccga
     4861 ggagggcgag aagatggtgc tggagaacaa tttctttgtg gagaccgtgc tgccttctaa
     4921 gatcatgaga aagctggagc cagaggagtt cgccgcctac ctggagccct ttaaggagaa
     4981 gggcgaggtg cgccggccaa cactgtcttg gcctagggag atcccactgg tgaagggcgg
     5041 caagcccgat gtggtgcaga tcgtgcgcaa ctacaatgcc tatctgcggg ccagcgacga
     5101 tctgccaaag ctgttcatcg agtccgaccc cggcttcttt tctaacgcca tcgtggaggg
     5161 cgccaagaag ttccctaata ccgagtttgt gaaggtgaag ggcctgcact ttctgcagga
     5221 ggacgcccca gatgagatgg gcaagtacat caagagcttc gtcgagcgcg tgctgaagaa
     5281 cgagcagagc ggaggaggag gctctaacaa tcaggagaat cggatgaagg agtccctggc
     5341 cctgtttggc acaatcctgg agctgccctg gttcaagtcc acctctgtga tcctgtttct
     5401 gaataagaca gacatcctgg aggagaagat ccctacctcc cacctggcca catatttccc
     5461 ctcttttcag ggacctaagc aggatgcaga ggcagcaaag agattcatcc tggacatgta
     5521 caccaggatg tatacaggat gcgtggacgg accagagggc agcaagaagg gagccagaag
     5581 cagaaggctg ttttcccact acacctgtgc caccgataca cagaacatca ggaaggtgtt
     5641 caaggacgtg agagattccg tgctggccag gtatctggac gagatcaatc tgctgtgagg
     5701 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
     5761 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     5821 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5881 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5941 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     6001 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     6061 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     6121 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     6181 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     6241 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     6301 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     6361 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     6421 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     6481 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     6541 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     6601 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     6661 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6721 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6781 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6841 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6901 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6961 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     7021 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     7081 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7141 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7201 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7261 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7321 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7381 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7441 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7501 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7561 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7621 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7681 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7741 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     7801 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7861 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7921 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7981 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     8041 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     8101 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     8161 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     8221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     9061 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     9121 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     9181 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     9241 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     9301 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     9361 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     9421 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     9481 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     9541 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     9601 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     9661 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     9721 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     9781 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     9841 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     9901 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     9961 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    10021 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    10081 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    10141 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    10201 gccacctgac gtc
//
