LOCUS       sequence-385874-       10240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNA12) with RLuc8, a G gamma subunit 
            (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10240)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Ga12
FEATURES             Location/Qualifiers
     source          1..10240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3007..3024)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3036..3609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3059..3611
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3203..3220)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3430..3449
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5831..5848)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5837..6061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6107..6535
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6194..6213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6404..6425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6544..6564)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6549..6878
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6729..6864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6791..6810
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6945..7739
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6999..7018)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7609..7628
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7913..8034
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7950..7969)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8004..8023
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8083..8099)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8083..8099)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8096..8118)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8107..8123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8131..8137,8138..8155,8156..8161))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8176..8197
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8314..8331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8485..9073)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8565..8584)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9244..10035,10036..10104))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9867..9886
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10105..10209)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
     2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca
     2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
     2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 cagcgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct
     2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga
     2701 tgagctgtac aagggcagcg ccggcaccat ggcacaggac ctgtccgaga aggatctgct
     2761 gaagatggag gtggagcagc tgaagaagga ggtgaagaat acaagaatcc ccatcagcaa
     2821 ggccggcaag gagatcaagg agtatgtgga ggcccaggcc ggcaacgacc ctttcctgaa
     2881 gggcatccca gaggataaga atccctttaa ggagaagggc ggctgcatca tcagctgacc
     2941 gcggcgtacg aggcctgcat gctccggacc tgcaggttcg aagtcgacag atctcaattg
     3001 gggcccctat agtgtcacct aaataattcc gcccccccct ctccctcccc cccccctaac
     3061 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3121 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3181 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3241 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3301 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3361 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3421 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3481 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3541 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3601 cgatgataat atgtccggcg tggtgagaac cctgtctagg tgcctgctgc cagcagaggc
     3661 aggaggagcc cgggagcgga gagccggcag cggcgcccgc gatgccgagc gggaggccag
     3721 gcgccggtcc agagacatcg atgccctgct ggccagggag agaagggccg tgcgcaggct
     3781 ggtgaagatc ctgctgctgg gagcaggaga gtctggcaag agcacctttc tgaagcagat
     3841 gagaatcatc cacggcaggg agttcgacca gaaggccctg ctggagttca gagacacaat
     3901 ctttgataac atcctgaagg gctccagagt gctggtggac gccagggata agctgggcat
     3961 cccctggcag tactccgaga atgaggagca cggcatgttc ctgatggcat ctggaggagg
     4021 aggctccatg gcatctaagg tgtatgaccc tgagcagaga aagaggatga tcaccggacc
     4081 acagtggtgg gcaaggtgca agcagatgaa cgtgctggat agctttatca attactatga
     4141 ctccgagaag cacgccgaga acgccgtgat cttcctgcac ggcaatgcca caagctccta
     4201 cctgtggagg cacgtggtgc cacacatcga gccagtggca aggtgtatca tccccgatct
     4261 gatcggcatg ggcaagagcg gcaagtccgg caacggcagc tatcgcctgc tggaccacta
     4321 caagtatctg acagcctggt tcgagctgct gaatctgcct aagaagatca tctttgtggg
     4381 acacgactgg ggagcagcac tggcattcca ctacgcctat gagcaccagg atcggatcaa
     4441 ggccatcgtg cacatggagt ctgtggtgga cgtgatcgag agctgggacg agtggcctga
     4501 catcgaggag gacatcgccc tgatcaagtc tgaggagggc gagaagatgg tgctggagaa
     4561 caatttcttt gtggagaccg tgctgcctag caagatcatg cgcaagctgg agccagagga
     4621 gttcgccgcc tacctggagc cctttaagga gaagggcgag gtgagaaggc ccacactgtc
     4681 ctggcctcgg gagatcccac tggtgaaggg cggcaagcct gatgtggtgc agatcgtgcg
     4741 caactacaat gcctatctgc gggcctctga cgatctgcca aagctgttca tcgagagcga
     4801 ccccggcttc ttttccaacg ccatcgtgga gggcgccaag aagttcccaa ataccgagtt
     4861 tgtgaaggtg aagggcctgc actttctgca ggaggacgcc cccgatgaga tgggcaagta
     4921 catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag agcggaggag gaggctcttt
     4981 tgagaataag gcaggactgc cagtggagcc tgcaacattc cagctgtatg tgccagccct
     5041 gtctgcactg tggagggata gcggcatccg ggaggccttc tctcgcagga gcgagtttca
     5101 gctgggcgag agcgtgaagt actttctgga caacctggat agaatcggcc agctgaatta
     5161 tttcccttcc aagcaggaca tcctgctggc caggaaggcc accaagggca tcgtggagca
     5221 cgatttcgtg atcaagaaga tccccttcaa gatggtggac gtgggaggac agaggtctca
     5281 gaggcagaag tggttccagt gctttgatgg catcacaagc atcctgttta tggtgtctag
     5341 ctccgagtac gatcaggtgc tgatggagga cagaaggacc aacagactgg tggagtccat
     5401 gaatatcttt gagacaatcg tgaacaataa gctgttcttt aacgtgtcta tcatcctgtt
     5461 cctgaataag atggacctgc tggtggagaa ggtgaagacc gtgtccatca agaagcactt
     5521 ccccgatttt agaggcgacc ctcacaggct ggaggatgtg cagagatacc tggtgcagtg
     5581 tttcgacagg aagaggagga acaggagcaa gcccctgttc caccacttta ccacagccat
     5641 cgataccgag aacgtgaggt tcgtgtttca cgccgtgaag gatacaatcc tgcaggagaa
     5701 tctgaaggac atcatgctgc agtgaggtac cgagctcgga tccactagtc cagtgtggtg
     5761 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
     5821 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5881 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5941 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6001 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6061 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6121 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6181 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6241 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6301 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6361 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6421 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6481 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     6541 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6601 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6661 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6721 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6781 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     6841 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     6901 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     6961 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7021 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7081 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     7141 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7201 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7261 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7321 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7381 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7441 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     7501 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7561 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7621 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7681 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     7741 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     7801 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7861 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7921 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7981 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     8041 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     8101 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     8161 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     8221 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     8281 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     8341 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     8401 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     8461 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     8521 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     8581 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     8641 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     8701 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     8761 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8821 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8881 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     8941 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     9001 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     9061 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     9121 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     9181 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     9241 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     9301 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     9361 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     9421 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     9481 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     9541 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     9601 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     9661 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     9721 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     9781 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     9841 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     9901 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     9961 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    10021 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    10081 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    10141 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    10201 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
