LOCUS       sequence-385881-       10228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit 
            (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components 
            of a BRET2 biosensor for studying heterotrimeric G proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, 
            Che T, Gavin AC, McCorvy JD, Roth BL, Strachan RT
  TITLE     TRUPATH, an open-source biosensor platform for interrogating the 
            GPCR transducerome.
  JOURNAL   Nat Chem Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8.
  PUBMED    32367019
REFERENCE   2  (bases 1 to 10228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2020 May 4. pii: 10.1038/s41589-020-0535-8. doi: 
            10.1038/s41589-020-0535-8."
COMMENT     Sequence Label: TRUPATH Triple Ga13
FEATURES             Location/Qualifiers
     source          1..10228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1937..1990
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(3007..3024)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    3036..3609
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3059..3611
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3203..3220)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3430..3449
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(5819..5836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5825..6049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6095..6523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6182..6201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6392..6413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6532..6552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6537..6866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6717..6852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6779..6798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6933..7727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6987..7006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7597..7616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7901..8022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7938..7957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7992..8011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8071..8087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8071..8087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8084..8106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8095..8111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8119..8125,8126..8143,8144..8149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8164..8185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8302..8319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8473..9061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8553..8572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9232..10023,10024..10092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9855..9874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10093..10197)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttatgg gagagatgga gcagctgagg caggaggcag agcagctgaa
      961 gaagcagatc gccgacgcca gaaaggcctg cgcagatgtg accctggcag agctggtgag
     1021 cggactggag gtggtgggaa gggtgcagat gaggaccagg agaacactga ggggccacct
     1081 ggccaagatc tacgccatgc actgggccac agactctaag ctgctggtgt ccgcctctca
     1141 ggacggcaag ctgatcgtgt gggatagcta taccacaaac aaggtgcacg caatccccct
     1201 gcgcagctcc tgggtcatga cctgtgccta cgccccttcc ggaaatttcg tggcatgcgg
     1261 aggactggat aacatgtgct ctatctataa tctgaagagc cgggagggca acgtgaaggt
     1321 gtctagagag ctgagcgccc acaccggcta cctgtcttgc tgtcggttcc tggacgataa
     1381 caatatcgtg acatctagcg gcgacaccac atgcgcactg tgggacatcg agaccggaca
     1441 gcagaagaca gtgtttgtgg gccacacagg cgactgtatg tccctggccg tgtctccaga
     1501 tttcaatctg tttatctccg gagcatgcga cgcatctgcc aagctgtggg atgtgcggga
     1561 gggcacctgt agacagacct tcacaggcca cgagagcgac atcaacgcca tctgcttctt
     1621 tcccaatggc gaggccatct gcacaggcag cgacgatgcc tcctgtaggc tgttcgacct
     1681 gcgcgccgat caggagctga tctgcttttc tcacgagagc atcatctgtg gcatcacctc
     1741 cgtggccttt agcctgtccg gcaggctgct gttcgcagga tatgacgact tcaactgcaa
     1801 cgtgtgggac tccatgaagt ctgagagggt gggcatcctg agcggccacg ataatcgcgt
     1861 gtcctgtctg ggagtgaccg cagacggaat ggcagtggcc acaggcagct gggattcctt
     1921 tctgaagatc tggaacgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa
     1981 tcccggccct accggtatgg tgtccaaggg agaggagctg ttcaccggag tggtgcctat
     2041 cctggtggag ctggacggcg atgtgaatgg ccacaagttt agcgtgtccg gagagggaga
     2101 gggcgacgca acctacggca agctgacact gaagttcatc tgcaccacag gcaagctgcc
     2161 cgtgccttgg ccaaccctgg tgaccacact gtcctacggc gtgcagtgtt tttctaggta
     2221 tcctgaccac atgaagcagc acgatttctt taagtctgcc atgccagagg gctacgtgca
     2281 ggagcggaca atcttcttta aggacgatgg caactataag accagagccg aggtgaagtt
     2341 cgagggcgac acactggtga acaggatcga gctgaagggc atcgacttta aggaggatgg
     2401 caatatcctg ggccacaagc tggagtacaa ctataatccc cacaacgtgt atatcatggc
     2461 cgataagcag aagaacggca tcaaggtcaa tttcaagatc cgccacaata tcgaggacgg
     2521 cagcgtgcag ctggccgatc actaccagca gaacacccca atcggcgacg gacccgtgct
     2581 gctgcctgat aatcactatc tgtttacaca gtctgccctg agcaaggacc ccaacgagaa
     2641 gcgggatcac atggtgctgc tggagttcgt gaccgcagca ggcatcacac tgggcatgga
     2701 tgagctgtac aagggcagcg ccggcaccat ggcacaggac ctgtccgaga aggatctgct
     2761 gaagatggag gtggagcagc tgaagaagga ggtgaagaat acaagaatcc ccatcagcaa
     2821 ggccggcaag gagatcaagg agtatgtgga ggcccaggcc ggcaacgacc ctttcctgaa
     2881 gggcatccca gaggataaga atccctttaa ggagaagggc ggctgcatca tcagctgacc
     2941 gcggcgtacg aggcctgcat gctccggacc tgcaggttcg aagtcgacag atctcaattg
     3001 gggcccctat agtgtcacct aaataattcc gcccccccct ctccctcccc cccccctaac
     3061 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3121 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3181 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3241 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     3301 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     3361 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     3421 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     3481 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     3541 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     3601 cgatgataat atggccgatt tcctgccatc tcgcagcgtg ctgtctgtgt gctttccagg
     3661 atgtctgctg accagcggag aggcagagca gcagcggaag agcaaggaga tcgacaagtg
     3721 cctgtccaga gagaagacct acgtgaagag gctggtgaag atcctgctgc tgggagcagg
     3781 agagagcggc aagtccacat ttctgaagca gatgagaatc atccacggcc aggacttcga
     3841 tcagagagcc agggaggagt ttaggccaac aatctattct aacgtgatca agggaatgag
     3901 ggtgctggtg gatgcccggg agaagctgca catcccatgg ggcgataaca gcaatcagca
     3961 gcacggcgac aagatgatgt ccttctctgg aggaggaggc tctatggcaa gcaaggtgta
     4021 cgaccctgag cagcgcaagc ggatgatcac cggcccacag tggtgggcca gatgcaagca
     4081 gatgaacgtg ctggattctt ttatcaatta ctatgacagc gagaagcacg ccgagaacgc
     4141 cgtgatcttc ctgcacggca atgccacaag ctcctacctg tggaggcacg tggtgcccca
     4201 catcgagcct gtggcaaggt gtatcatccc cgatctgatc ggcatgggca agtccggcaa
     4261 gtctggcaac ggctcctatc gcctgctgga ccactacaag tatctgaccg cctggttcga
     4321 gctgctgaat ctgcctaaga agatcatctt tgtgggacac gactggggag cagcactggc
     4381 attccactac gcctatgagc accaggatcg gatcaaggcc atcgtgcaca tggagtccgt
     4441 ggtggacgtg atcgagtctt gggacgagtg gcccgacatc gaggaggaca tcgccctgat
     4501 caagagcgag gagggcgaga agatggtgct ggagaacaat ttctttgtgg agaccgtgct
     4561 gccatccaag atcatgagaa agctggagcc agaggagttc gcagcatacc tggagccttt
     4621 taaggagaag ggcgaggtgc ggagaccaac actgtcctgg ccaagggaga tccccctggt
     4681 gaagggcggc aagcccgatg tggtgcagat cgtgcgcaac tacaatgcct atctgcgggc
     4741 ctctgacgat ctgcccaagc tgtttatcga gagcgaccct ggcttctttt ccaacgccat
     4801 cgtggagggc gccaagaagt tccccaatac cgagtttgtg aaggtgaagg gcctgcactt
     4861 tctgcaggag gacgcccctg atgagatggg caagtatatc aagagcttcg tggagcgcgt
     4921 gctgaagaac gagcagagcg gaggaggagg ctctgatacc cgggccccta tggcagcaca
     4981 gggaatggtg gagacaagag tgttcctgca gtacctgcca gcaatcaggg cactgtgggc
     5041 agattccggc atccagaacg cctatgacag gaggagggag ttccagctgg gagagtctgt
     5101 gaagtacttt ctggacaatc tggataagct gggcgagcct gattatatcc catcccagca
     5161 ggacatcctg ctggccagaa ggcccaccaa gggcatccac gagtacgatt tcgagatcaa
     5221 gaatgtgcct tttaagatgg tggacgtggg cggccagaga agcgagagaa agaggtggtt
     5281 cgagtgtttt gacagcgtga catccatcct gttcctggtg tctagctccg agtttgatca
     5341 ggtgctgatg gaggaccgcc tgaccaaccg gctgacagag tccctgaata tcttcgagac
     5401 catcgtgaac aatagggtgt tctctaacgt gagcatcatc ctgtttctga ataagacaga
     5461 tctgctggag gagaaggtgc agatcgtgag catcaaggac tacttcctgg agtttgaggg
     5521 cgatcctcac tgcctgagag acgtgcagaa gttcctggtg gagtgtttta ggaacaagcg
     5581 ccgggatcag cagcagaagc cactgtatca ccactttacc acagccatca acaccgagaa
     5641 tatcagactg gtgttcaggg acgtgaagga tacaatcctg cacgacaatc tgaagcagct
     5701 gatgctgcag tgaggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga
     5761 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc
     5821 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5881 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5941 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6001 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6061 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6121 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6181 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6241 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6301 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6361 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6421 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6481 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6541 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6601 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6661 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6721 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6781 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6841 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6901 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     6961 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     7021 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7081 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7141 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7201 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7261 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7321 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7381 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7441 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7501 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7561 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7621 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7681 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     7741 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     7801 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     7861 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     8041 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     8101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     8161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     8341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     8401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     9001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     9061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     9121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     9181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     9241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     9301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     9361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    10021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    10081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    10141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    10201 atttccccga aaagtgccac ctgacgtc
//
