LOCUS       sequence-385887-        8252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid containing promoter of RecA from Xanthomonas oryzae driving 
            expression of dead Cas9, cytosine deaminase CDA1, and glycosylase 
            inhibitor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, 
            Pauwels L, White FF, Dong H, Yang B
  TITLE     Efficient CRISPR-Cas9 based cytosine base editors for 
            phytopathogenic bacteria.
  JOURNAL   Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8.
  PUBMED    36646768
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-01-17"; volume: "6(1):56. doi"; pages: " 
            10.1038/s42003-023-04451-8"
COMMENT     Sequence Label: pEN-dCas9-CDA1-UGI
FEATURES             Location/Qualifiers
     source          1..8252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      125..152
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(311..328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(482..1070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(562..581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1217..2026)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1940..1959
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2018..2037
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2160..2179
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2160..2178
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2251..2374)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2451..2702)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(2709..3332)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(3339..3404)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(3423..3488)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(join(3696..5279,5280..5282,5283..7769,7770..7772,
                     7773..7799))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(7983..8000)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7983..7999)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7992..8014)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(8052..8147)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      join(8199..8252,1..33)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgtg
       61 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaact
      121 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctggctag
      181 cggtacgcgt attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1081 tgatcttttc tacggggtct gacgctcagt ggaacgacgc gtaactcacg ttaagggatt
     1141 ttggtcatga gcttgcgccg tcccgtcaag tcagcgtaat gctctgccag tgttacaacc
     1201 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     1261 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     1321 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     1381 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     1441 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     1501 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     1561 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     1621 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     1681 cacctgaatc aggatattct tctaatacct ggaatgctgt ttttccgggg atcgcagtgg
     1741 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     1801 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     1861 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaagcga tagattgtcg
     1921 cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt
     1981 tggaatttaa tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat
     2041 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     2101 tgtaacatca gagattttga gacacgggcc agagctgcca ggaaacagct atgaccatgt
     2161 aatacgactc actatagggg atatcgcggc cgccctgcag ctctagagct cgaattctac
     2221 aggtcactaa taccatctaa gtagttggtt catagtgact gcatatgttg tgttttacag
     2281 tattatgtag tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt
     2341 tacgtttctc gttcaacttt tttgtacaaa cttgcatact gcaaaaaacc cctcagactc
     2401 ctaaagaggg aacccaaggg gtttgcttat tctgattatg caaccagtcc tagcatcttg
     2461 atcttgttct ctccattaga gtcctgaatc accagagccc agggtttgta ctctggggca
     2521 tctgacgtga gcagcatcac gttctcatcg gtgctctcat catacgcagt gtgtacgaga
     2581 atgtcacttt ccggcttatt cccaatgacc tcctcaactt cttcaggaag catgagtatc
     2641 gactcttgga tgaccaactg tttgcctgtt tccttctcta tgatgtcgga aaggttggtc
     2701 attctagaaa cagcaggact cttagtggtg tggagtattt ttacctgaat cataatggac
     2761 aactcgctcc gtcgtttttc agctcgcttc aaagtcttct caagccatct attctcattc
     2821 aattgattgt gcgacgattg gatgaatatt ttcctgcaac attggtagtg ttcacttacc
     2881 attacattca acccaacccc gttatctctg aggttccaca gcccaatttg attcctcgca
     2941 tttttctcgt aatagagttt gcaagcccag attttcaaag tgtggccgtt cccccgcagc
     3001 tcctggttat accattctaa gatcttttca gcgcaatctg cacaaggact ccaggatgag
     3061 taccaattta tcgtgaattg tccggggttg tcgcgcaggt attcttcgac ttttctaatg
     3121 ctaaagattt cggcgtgaat gccacgttct gtcccgctct gtggtttatt cacagcatag
     3181 ccccaaaaac acgctctacg ttcaccccgt cgttttaatt caaagagaac gtagcatcta
     3241 tgcgacacgg attttttgtt gttgaaaaac tgtttcttaa acgtgtagat gtccaacttc
     3301 tcatggattc tcacgtactc agcgtcggtc atcctagact tatcgtcatc gtctttgtaa
     3361 tcaatatcat gatccttgta gtctccgtcg tggtccttat agtctccgga ctcgagccta
     3421 gacttatcgt catcgtcttt gtaatcaata tcatgatcct tgtagtctcc gtcgtggtcc
     3481 ttatagtctc cggaatactt ctccacgtaa gggacaggaa tcatccccct ctttccttcg
     3541 ctgtcctctg cattccacca ctgctcctca ggcttatccc ggattctcag gatgtctcct
     3601 ttcttaaagg gaagatcctc ttcatcattc ccattaaagt caaagagggc tcgcacatac
     3661 tcagcagaac ctccacctcc agaacctcct ccaccgtcac ctcctagctg actcaaatca
     3721 atgcgtgttt cataaagacc agtgatggat tgatggataa gagtggcatc taaaacttct
     3781 tttgtagacg tatatcgttt acgatcaatt gttgtatcaa aatatttaaa agcagcggga
     3841 gctccaagat tcgtcaacgt aaataaatga ataatatttt ctgcttgttc acgtattggt
     3901 ttgtctctat gtttgttata tgcactaaga actttatcta aattggcatc tgctaaaata
     3961 acacgcttag aaaattcact gatttgctca ataatctcat ctaaataatg cttatgctgc
     4021 tccacaaaca attgtttttg ttcgttatct tctggactac ccttcaactt ttcataatga
     4081 ctagctaaat ataaaaaatt cacatatttg cttggcagag ccagctcatt tcctttttgt
     4141 aattctccgg cactagccag catccgttta cgaccgtttt ctaactcaaa aagactatat
     4201 ttaggtagtt taatgattaa gtctttttta acttccttat atcctttagc ttctaaaaag
     4261 tcaatcggat ttttttcaaa ggaacttctt tccataattg tgatccctag taactcttta
     4321 acggatttta acttcttcga tttccctttt tccaccttag caaccactag gactgaataa
     4381 gctaccgttg gactatcaaa accaccatat ttttttggat cccagtcttt tttacgagca
     4441 ataagcttgt ccgaatttct ttttggtaaa attgactcct tggagaatcc gcctgtctgt
     4501 acttctgttt tcttgacaat attgacttgg ggcatggaca atactttgcg cactgtggca
     4561 aaatctcgcc ctttatccca gacaatttct ccagtttccc cattagtttc gattagaggg
     4621 cgtttgcgaa tctctccatt tgcaagtgta atttctgttt tgaagaagtt catgatatta
     4681 gagtaaaaga aatattttgc ggttgctttg cctatttctt gctcagactt agcaatcatt
     4741 ttacgaacat cataaacttt ataatcacca tagacaaact ccgattcaag ttttggatat
     4801 ttcttaatca aagcagttcc aacgacggca tttagatacg catcatgggc atgatggtaa
     4861 ttgttaatct cacgtacttt atagaattgg aaatcttttc ggaagtcaga aactaattta
     4921 gattttaagg taatcacttt aacctctcga ataagtttat cattttcatc gtatttagta
     4981 ttcatgcgac tatccaaaat ttgtgccaca tgcttagtga tttggcgagt ttcaaccaat
     5041 tggcgtttga taaaaccagc tttatcaagt tcactcaaac ctccacgttc agctttcgtt
     5101 aaattatcaa acttacgttg agtgattaac ttggcgttta gaagttgtct ccaatagttt
     5161 ttcatctttt tgactacttc ttcacttgga acgttatccg atttaccacg atttttatca
     5221 gaacgcgtta agaccttatt gtctattgaa tcgtctttaa ggaaactttg tggaacaatg
     5281 gcatcgacat cataatcact taaacgatta atatctaatt cttggtccac atacatgtct
     5341 cttccatttt ggagataata gagatagagc ttttcatttt gcaattgagt attttcaaca
     5401 ggatgctctt taagaatctg acttcctaat tctttgatac cttcttcgat tcgtttcata
     5461 cgctctcgcg aatttttctg gcccttttga gttgtctgat tttcacgtgc catttcaata
     5521 acgatatttt ctggcttatg ccgccccatt actttgacca attcatcaac aacttttaca
     5581 gtctgtaaaa tacctttttt aatagcaggg ctaccagcta aatttgcaat atgttcatgt
     5641 aaactatcgc cttgtccaga cacttgtgct ttttgaatgt cttctttaaa tgtcaaacta
     5701 tcatcatgga tcagctgcat aaaattgcga ttggcaaaac catctgattt caaaaaatct
     5761 aatattgttt tgccagattg cttatcccta ataccattaa tcaattttcg agacaaacgt
     5821 ccccaaccag tataacggcg acgtttaagc tgtttcatca ccttatcatc aaagaggtga
     5881 gcatatgttt taagtctttc ctcaatcatc tccctatctt caaataaggt caatgttaaa
     5941 acaatatcct ctaagatatc ttcattttct tcattatcca aaaaatcttt atctttaata
     6001 atttttagca aatcatggta ggtacctaat gaagcattaa atctatcttc aactcctgaa
     6061 atttcaacac tatcaaaaca ttctattttt ttgaaataat cttcttttaa ttgcttaacg
     6121 gttacttttc gatttgtttt gaagagtaaa tcaacaatgg ctttcttctg ttcacctgaa
     6181 agaaatgctg gttttcgcat tccttcagta acatatttga cctttgtcaa ttcgttataa
     6241 accgtaaaat actcataaag caaactatgt tttggtagta ctttttcatt tggaagattt
     6301 ttatcaaagt ttgtcatgcg ttcaataaat gattgagctg aagcaccttt atcgacaact
     6361 tcttcaaaat tccatggggt aattgtttct tcagacttcc gagtcatcca tgcaaaacga
     6421 ctattgccac gcgccaatgg accaacataa taaggaattc gaaaagtcaa gattttttca
     6481 atcttctcac gattgtcttt taaaaatgga taaaagtctt cttgtcttct caaaatagca
     6541 tgcagctcac ccaagtgaat ttgatgggga atagagccgt tgtcaaaggt ccgttgcttg
     6601 cgcagcaaat cttcacgatt tagtttcacc aataattcct cagtaccatc cattttttct
     6661 aaaattggtt tgataaattt ataaaattct tcttggctag ctcccccatc aatataacct
     6721 gcatatccgt tttttgattg atcaaaaaag atttctttat acttttctgg aagttgttgt
     6781 cgaactaaag cttttaaaag agtcaagtct tgatgatgtt catcgtagcg tttaatcatt
     6841 gaagctgata ggggagcctt agttatttca gtatttactc ttaggatatc tgaaagtaaa
     6901 atagcatctg ataaattctt agctgccaaa aacaaatcag catattgatc tccaatttgc
     6961 gccaataaat tatctaaatc atcatcgtaa gtatcttttg aaagctgtaa tttagcatct
     7021 tctgccaaat caaaatttga tttaaaatta ggggtcaaac ccaatgacaa agcaatgaga
     7081 ttcccaaata agccattttt cttctcaccg gggagctgag caatgagatt ttctaatcgt
     7141 cttgatttac tcaatcgtgc agaaagaatc gctttagcat ctactccact tgcgttaata
     7201 gggttttctt caaataattg attgtaggtt tgtaccaact ggataaatag tttgtccaca
     7261 tcactattat caggatttaa atctccctca atcaaaaaat gaccacgaaa cttaatcata
     7321 tgcgctaagg ccaaatagat taagcgcaaa tccgctttat cagtagaatc taccaatttt
     7381 tttcgcagat gatagatagt tggatatttc tcatgataag caacttcatc tactatattt
     7441 ccaaaaatag gatgacgttc atgcttcttg tcttcttcca ccaaaaaaga ctcttcaagt
     7501 cgatgaaaga aactatcatc tactttcgcc atctcatttg aaaaaatctc ctgtagataa
     7561 caaatacgat tcttccgacg tgtatacctt ctacgagctg tccgtttgag acgagtcgct
     7621 tccgctgtct ctccactgtc aaataaaaga gcccctataa gatttttttt gatactgtgg
     7681 cggtctgtat ttcccagaac cttgaacttt ttagacggaa ccttatattc atcagtgatc
     7741 accgcccatc cgacgctatt tgtgccgata gctaagccta ttgagtattt cttatccatc
     7801 tgggtgatcc tcgtcagtga tttcggtatg gggctaatgt agcggcggtg tatcgcacag
     7861 gctgagactg caggcgacac actcatatta gggagacgcc atgtcccgcg gcagggggaa
     7921 atctgcggtg cctgctcggg aaaaagccag cacgaccatc ggtacagagc tcattactta
     7981 agactggccg tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttactagt
     8041 gctcgaggta gcaacttttc tatacaaagt tggcattata aaaaagcatt gcttatcaat
     8101 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt
     8161 aagatctgtt aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     8221 cggaagactg ggcctttcgt tttatctgtt gt
//
