LOCUS       sequence-385895-       10168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10168)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0277
FEATURES             Location/Qualifiers
     source          1..10168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3106..3694
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3159..3179)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3577..3588)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3766..3999
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4077..4198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4114..4133)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4168..4187
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4238..4373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4393..4412)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4394..4412)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4422..4439)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4422..4438)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4431..4453)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4580..5035
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4667..4686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4877..4898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5061..5165
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5166..5234,5235..6026)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5384..5403)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6197..6785
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6686..6705
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6939..6956
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7073..7094
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7109..7114,7115..7132,7133..7139)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7147..7163
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7152..7174
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7171..7187
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7171..7187
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7206..7226
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7208..7226
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7254..7480
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7481..7661
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7708..7833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8326..8559
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8744..8788
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9019..9229
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9041..9237
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9088..9223
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9269..9871
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9269..9288)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9765..9785
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9914..10031
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ccagccgcag aggatggcgc ctgtgggcac agacaaggag ctcagtgacc tcctggactt
     1261 cagcatgatg ttcccgctgc ctgtcaccaa cgggaagggc cggcccgcct ccctggccgg
     1321 ggcgcagttc ggaggttcag gcaagagcgg tgagcggggc gcctatgcct ccttcgggag
     1381 agacgcaggc gtgggcggcc tgactcaggc tggcttcctg tcaggcgagc tggccctcaa
     1441 cagccccggg cccctgtccc cttcgggcat gaaggggacc tcccagtact acccctccta
     1501 ctccggcagc tcccggcgga gagcggcaga cggcagccta gacacgcagc ccaagaaggt
     1561 ccggaaggtc ccgccgggtc ttccatcctc ggtgtaccca cccagctcag gtgaggacta
     1621 cggcagggat gccaccgcct acccgtccgc caagaccccc agcagcacct atcccgcccc
     1681 cttctacgtg gcagatggca gcctgcaccc ctcagccgag ctctggagtc ccccgggcca
     1741 ggcgggcttc gggcccatgc tgggtggggg ctcatccccg ctgcccctcc cgcccggtag
     1801 cggcccggtg ggcagcagtg gaagcagcag cacgtttggt ggcctgcacc agcacgagcg
     1861 tatgggctac cagctgcatg gagcagaggt gaacggtggg ctcccatctg catcctcctt
     1921 ctcctcagcc cccggagcca cgtacggcgg cgtctccagc cacacgccgc ctgtcagcgg
     1981 ggccgacagc ctcctgggct cccgagggac cacagctggc agctccgggg atgccctcgg
     2041 caaagcactg gcctcgatct actccccgga tcactcaagc aataacttct cgtccagccc
     2101 ttctaccccc gtgggctccc cccagggcct ggcaggaacg tcacagtggc ctcgagcagg
     2161 agcccccggt gccttatcgc ccagctacga cgggggtctc cacggcctgc agagtaagat
     2221 agaagaccac ctggacgagg ccatccacgt gctccgcagc cacgccgtgg gcacagccgg
     2281 cgacatgcac acgctgctgc ctggccacgg ggcgctggcc tcaggtttca ccggccccat
     2341 gtcactgggc gggcggcacg caggcctggt tggaggcagc caccccgagg acggcctcgc
     2401 aggcagcacc agcctcatgc acaaccacgc ggccctcccc agccagccag gcaccctccc
     2461 tgacctgtct cggcctcccg actcctacag tgggctaggg cgagcaggtg ccacggcggc
     2521 cgccagcgag atcaagcggg aggagaagga ggacgaggag aacacgtcag cggctgacca
     2581 ctcggaggag gagaagaagg agctgaaggc cccccgggcc cggaccagca gtacggacga
     2641 ggtgctgtcc ctggaggaga aagacctgag ggaccgggag aggcgcatgg ccaataacgc
     2701 gcgggagcgg gtgcgcgtgc gggatattaa cgaggccttc cgggagctgg ggcgcatgtg
     2761 ccagatgcac ctcaagtcgg acaaagcgca gaccaagctg ctcatcctgc agcaggccgt
     2821 gcaggtcatc ctggggctgg agcagcaggt gcgagagcgg aacctgaatc ccaaagcagc
     2881 ctgtttgaaa cggcgagaag aggaaaaggt gtcaggtgtg gttggagacc cccagatggt
     2941 gctttcagct ccccacccag gcctgagcga agcccacaac cccgccgggc acatgtagta
     3001 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     3061 ggacgaatct cggctccacg atgtctgacg acgcgttaag tcgacaatca acctctggat
     3121 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3181 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3241 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3301 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3361 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3421 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3481 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3541 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3601 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3661 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     3721 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3781 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3841 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3901 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3961 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     4021 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     4081 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4141 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4201 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     4261 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     4321 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     4381 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     4441 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4501 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4561 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4621 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4681 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4741 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4801 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4861 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4921 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4981 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     5041 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5101 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5161 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5221 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5281 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5341 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5401 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5461 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5521 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5581 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5641 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5701 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5761 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5821 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5881 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5941 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     6001 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6061 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6121 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6181 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6241 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6301 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6361 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6421 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6481 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6541 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6601 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6661 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6721 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6781 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6841 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6901 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6961 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     7021 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7081 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7141 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7201 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     7261 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7321 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7381 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7441 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7501 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7561 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7621 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7681 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7741 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7801 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7861 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     7921 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     7981 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8041 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8101 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8161 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     8221 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8281 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8341 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8401 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8461 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8521 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8581 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8641 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8701 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8761 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     8821 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     8881 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     8941 cctcccaacc ccgaggggac ccatgcattg catctcaatt agtcagcaac caggtgtgga
     9001 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcgtctcaa ttagtcagca
     9061 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9121 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     9181 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9241 ctttctagag gtaccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     9301 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     9361 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     9421 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     9481 cgccgtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     9541 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     9601 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     9661 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     9721 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     9781 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     9841 gtgcatgacc cgcaagcccg gtgccagtta acgaattcta gatcttgaga caaatggcag
     9901 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     9961 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10021 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10081 cctgcaggtg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
    10141 cttctaggtc ttgaaaggag tgggaatt
//
