LOCUS       sequence-385898-        9421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3482
FEATURES             Location/Qualifiers
     source          1..9421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2175..2199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2207..2248
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2359..2947
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2412..2432)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2830..2841)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3019..3252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3330..3451
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3367..3386)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3421..3440
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3491..3626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3646..3665)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3647..3665)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3675..3692)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3675..3691)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3684..3706)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3833..4288
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3920..3939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4130..4151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4314..4418
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4419..4487,4488..5279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4637..4656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5450..6038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5939..5958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6192..6209
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6362..6367,6368..6385,6386..6392)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6400..6416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6405..6427
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6424..6440
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6424..6440
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6459..6479
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6461..6479
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6507..6733
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6734..6914
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6961..7086
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7579..7812
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7997..8041
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8272..8482
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8294..8490
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8341..8476
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8522..9124
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8522..8541)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9018..9038
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9167..9284
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcatcg attgatcaac
     1201 aagtttgtac aaaaaagttg gcatgtacaa catgatggag acggagctga agccgccggg
     1261 cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg ccggcggcaa
     1321 ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg
     1381 cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa
     1441 gcgcctgggc gccgagtgga aacttttgtc ggagacggag aagcggccgt tcatcgacga
     1501 ggctaagcgg ctgcgagcgc tgcacatgaa ggagcacccg gattataaat accggccccg
     1561 gcggaaaacc aagacgctca tgaagaagga taagtacacg ctgcccggcg ggctgctggc
     1621 ccccggcggc aatagcatgg cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt
     1681 gaaccagcgc atggacagtt acgcgcacat gaacggctgg agcaacggca gctacagcat
     1741 gatgcaggac cagctgggct acccgcagca cccgggcctc aatgcgcacg gcgcagcgca
     1801 gatgcagccc atgcaccgct acgacgtgag cgccctgcag tacaactcca tgaccagctc
     1861 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc
     1921 tggcatggct cttggctcca tgggttcggt ggtcaagtcc gaggccagct ccagcccccc
     1981 tgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat
     2041 gatcagcatg tatctccccg gcgccgaggt gccggaaccc gccgccccca gcagacttca
     2101 catgtcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc
     2161 cctctcacac atgtgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc
     2221 taaccctctc ctcggtctcg attctacgta gtaatgaact agtccgtaac ttgaaagtat
     2281 ttcgatttct tggctttata tatcttgtgg aaaggacgag ctgtgattag gcttcacagg
     2341 attacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2401 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2461 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2521 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2581 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2641 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2701 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2761 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     2821 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2881 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2941 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     3001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     3061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     3121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     3181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     3241 aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat ttataacttg
     3301 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     3361 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3421 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     3481 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     3541 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3601 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     3661 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     3721 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     3781 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     3841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     3901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     3961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     4081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     4201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4261 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     4321 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4381 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4441 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4501 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4561 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4621 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4681 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4741 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4801 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4861 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4921 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     4981 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5041 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5101 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5161 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5221 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5281 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5341 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5401 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5461 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5521 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5581 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5641 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5701 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5761 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5821 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5881 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5941 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6001 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6061 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6121 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6181 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6241 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6301 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6361 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6421 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6481 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6541 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6601 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6661 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6721 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6781 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6841 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6901 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     6961 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7021 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7081 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7141 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7201 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7261 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7321 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7381 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7441 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7501 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7561 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7621 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7681 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7741 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7801 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7861 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7921 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7981 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8041 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8101 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8161 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccatgca
     8221 ttgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8281 agtatgcaaa gcatgcgtct caattagtca gcaaccatag tcccgcccct aactccgccc
     8341 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8401 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8461 ggcttttttg gaggcctagg cttttgcaaa aagctttcta gaggtaccac cggagcttac
     8521 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     8581 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     8641 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     8701 catcggcaag gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga
     8761 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     8821 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     8881 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     8941 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     9001 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     9061 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccag
     9121 ttaacgaatt ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     9181 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     9241 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     9301 acagcagaga tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt
     9361 ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat
     9421 t
//
