LOCUS       sequence-385902-       10537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10537)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1974
FEATURES             Location/Qualifiers
     source          1..10537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3120..3129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3475..4063
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3528..3548)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3946..3957)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4135..4368
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4446..4567
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4483..4502)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4537..4556
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4607..4742
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4762..4781)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4763..4781)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4791..4808)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4791..4807)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4800..4822)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4949..5404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5036..5055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5246..5267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5430..5534
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5535..5603,5604..6395)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5753..5772)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6566..7154
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7055..7074
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7308..7325
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7442..7463
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7478..7483,7484..7501,7502..7508)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7516..7532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7521..7543
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7540..7556
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7540..7556
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7575..7595
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7577..7595
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7623..7849
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7850..8030
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8077..8202
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8695..8928
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9113..9157
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9388..9598
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9410..9606
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9457..9592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9638..10240
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9638..9657)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10134..10154
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10283..10400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgag
     1201 cctctccatg agagatccgg tcattcctgg gacaagcatg gcctaccatc cgttcctacc
     1261 tcaccgggcg ccggacttcg ccatgagcgc ggtgctgggt caccagccgc cgttcttccc
     1321 cgcgctgacg ctgcctccca acggcgcggc ggcgctctcg ctgccgggcg ccctggccaa
     1381 gccgatcatg gatcaattgg tgggggcggc cgagaccggc atcccgttct cctccctggg
     1441 gccccaggcg catctgaggc ctttgaagac catggagccc gaagaagagg tggaggacga
     1501 ccccaaggtg cacctggagg ctaaagaact ttgggatcag tttcacaagc ggggcaccga
     1561 gatggtcatt accaagtcgg gaaggcgaat gtttcctcca tttaaagtga gatgttctgg
     1621 gctggataaa aaagccaaat acattttatt gatggacatt atagctgctg atgactgtcg
     1681 ttataaattt cacaattctc ggtggatggt ggctggtaag gccgaccccg aaatgccaaa
     1741 gaggatgtac attcacccgg acagccccgc tactggggaa cagtggatgt ccaaagtcgt
     1801 cactttccac aaactgaaac tcaccaacaa catttcagac aaacatggat ttactatatt
     1861 gaactccatg cacaaatacc agccccggtt ccacattgta agagccaatg acatcttgaa
     1921 actcccttat agtacatttc ggacatactt gttccccgaa actgaattca tcgctgtgac
     1981 tgcataccag aatgataaga taacccagtt aaaaatagac aacaaccctt ttgcaaaagg
     2041 tttccgggac actggaaatg gccgaagaga aaaaagaaaa cagctcaccc tgcagtccat
     2101 gagggtgttt gatgaaagac acaaaaagga gaatgggacc tctgatgagt cctccagtga
     2161 acaagcagct ttcaactgct tcgcccaggc ttcttctcca gccgcctcca ctgtagggac
     2221 atcgaacctc aaagatttat gtcccagcga gggtgagagc gacgccgagg ccgagagcaa
     2281 agaggagcat ggccccgagg cctgcgacgc ggccaagatc tccaccacca cgtcggagga
     2341 gccctgccgt gacaagggca gccccgcggt caaggctcac cttttcgctg ctgagcggcc
     2401 ccgggacagc gggcggctgg acaaagcgtc gcccgactca cgccatagcc ccgccaccat
     2461 ctcgtccagc actcgcggcc tgggcgcgga ggagcgcagg agcccggttc gcgagggcac
     2521 agcgccggcc aaggtggaag aggcgcgcgc gctcccgggc aaggaggcct tcgcgccgct
     2581 cacggtgcag acggacgcgg ccgccgcgca cctggcccag ggccccctgc ctggcctcgg
     2641 cttcgccccg ggcctggcgg gccaacagtt cttcaacggg cacccgctct tcctgcaccc
     2701 cagccagttt gccatggggg gcgccttctc cagcatggcg gccgctggca tgggtcccct
     2761 cctggccacg gtttctgggg cctccaccgg tgtctcgggc ctggattcca cggccatggc
     2821 ctctgccgct gcggcgcagg gactgtccgg ggcgtccgcg gccaccctgc ccttccacct
     2881 ccagcagcac gtcctggcct ctcagggcct ggccatgtcc cctttcggaa gcctgttccc
     2941 ttacccctac acgtacatgg ccgcagcggc ggccgcctcc tctgcggcag cctccagctc
     3001 ggtgcaccgc caccccttcc tcaatctgaa caccatgcgc ccgcggctgc gctacagccc
     3061 ctactccatc ccggtgccgg tcccggacgg cagcagtctg ctcaccaccg ccctgccctc
     3121 catggcggcg gccgcggggc ccctggacgg caaagtcgcc gccctggccg ccagcccggc
     3181 ctcggtggca gtggactcgg gctctgaact caacagccgc tcctccacgc tctcctccag
     3241 ctccatgtcc ttgtcgccca aactctgcgc ggagaaagag gcggccacca gcgaactgca
     3301 gagcatccag cggttggtta gcggcttgga agccaagccg gacaggtccc gcagcgcgtc
     3361 cccgtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     3421 ttgtggaaag gacgacaccc tcggatcatt tgggataata cgcgttaagt cgacaatcaa
     3481 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3541 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3601 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     3661 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3721 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3781 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3841 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3901 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3961 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4021 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     4081 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4141 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4201 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4261 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4321 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     4381 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4441 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     4501 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     4561 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     4621 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4681 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4741 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4801 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4861 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4921 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4981 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5041 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5101 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5161 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5221 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5281 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5341 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5401 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     5461 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5521 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5581 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5641 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5701 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5761 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5821 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5881 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5941 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6001 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6061 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6121 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6181 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6241 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6301 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6361 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6421 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6481 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6541 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6601 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6661 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6721 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6781 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6841 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6901 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6961 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7021 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7081 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7141 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7201 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7261 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7321 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     7501 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     7561 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     7621 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     7681 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7741 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7801 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     8341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     8401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     8461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     8521 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     8581 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     8641 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     8701 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8761 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8821 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8881 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8941 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9001 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9061 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9121 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     9181 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     9241 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     9301 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     9361 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     9421 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     9481 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9541 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     9601 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     9661 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     9721 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9781 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9841 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9901 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9961 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
    10021 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
    10081 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
    10141 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
    10201 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
    10261 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10321 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10381 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
    10441 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
    10501 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
