LOCUS       sequence-385909-        9562 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1978
FEATURES             Location/Qualifiers
     source          1..9562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2500..3088
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2553..2573)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2971..2982)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3160..3393
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3471..3592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3508..3527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3562..3581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3632..3767
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3787..3806)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3788..3806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3816..3833)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3816..3832)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3825..3847)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3974..4429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4061..4080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4271..4292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4455..4559
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4560..4628,4629..5420)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4778..4797)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5591..6179
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6080..6099
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6333..6350
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6467..6488
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6503..6508,6509..6526,6527..6533)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6541..6557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6546..6568
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6565..6581
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6565..6581
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6600..6620
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6602..6620
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6648..6874
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6875..7055
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7102..7227
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7720..7953
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8138..8182
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8413..8623
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8435..8631
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8482..8617
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8663..9265
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8663..8682)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9159..9179
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9308..9425
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgca
     1201 cttcagcacc gtcaccaggg acatggaagc cttcacggcc agcagcctga gcagcctggg
     1261 ggccgcgggg ggcttcccgg gcgccgcgtc gcccggcgcc gacccgtacg gcccgcgcga
     1321 gcccccgccg ccgccgccgc gctacgaccc gtgcgccgcc gccgcccccg gcgccccggg
     1381 cccgccgccg ccgccgcacg cctacccgtt tgcgccggcc gccggggccg ccaccagcgc
     1441 cgccgccgag cccgagggcc ccggggccag ctgcgcggcc gcagccaagg cgccggtgaa
     1501 gaagaacgcg aaggtggccg gtgtgagcgt gcagctagag atgaaggcgc tgtgggacga
     1561 gttcaaccag ctgggcaccg agatgatcgt caccaaggcc ggcaggcgga tgtttcccac
     1621 cttccaagtg aagctcttcg gcatggatcc catggccgac tatatgctgc tcatggactt
     1681 cgtgccggtg gacgataagc gctaccggta cgccttccac agctcctcct ggctggtggc
     1741 ggggaaggcc gaccctgcca cgccaggccg cgtgcactac cacccggact cgcctgccaa
     1801 gggcgcgcag tggatgaagc aaatcgtgtc cttcgacaag ctcaagctga ccaacaacct
     1861 actggacgac aacggccaca ttattctgaa ttccatgcac agataccagc cccgcttcca
     1921 cgtggtctat gtggacccac gcaaagatag cgagaaatat gccgaggaga acttcaaaac
     1981 ctttgtgttc gaggagacac gattcaccgc ggtcactgcc taccagaacc atcggatcac
     2041 gcagctcaag attgccagca atcccttcgc gaaaggcttc cgggactgtg accctgagga
     2101 ctggccccgg aaccaccggc ccggcgcact gccgctcatg agcgccttcg cgcgctcgcg
     2161 gaaccccgtg gcttccccga cgcagcccag cggcacggag aaaggtggac atgtcctgaa
     2221 ggacaaggaa gtgaaagctg agacgtctag gaacacacca gagagagaag tggagcttct
     2281 gagggatgca ggtggctgtg tgaacctggg gctcccctgc cccgcagagt gccaaccctt
     2341 caatacccag ggcctggtgg ctgggaggac cgcaggtgac cgtctttgtt agtaatgaac
     2401 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     2461 ccatttggtg cttgctatga cgagacgcgt taagtcgaca atcaacctct ggattacaaa
     2521 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2581 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2641 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     2701 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     2761 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     2821 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     2881 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     2941 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3001 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3061 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3121 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3181 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     3241 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3301 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3361 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     3421 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3601 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3661 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3721 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3781 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3841 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3901 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3961 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4801 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4921 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4981 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5041 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5101 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5161 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5221 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5281 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5341 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5401 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5701 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6301 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6361 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6421 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6481 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6541 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6601 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6661 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6721 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6781 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6841 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6901 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6961 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7021 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7081 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7141 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7201 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7261 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7321 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7381 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7441 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7501 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7561 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7621 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7681 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7741 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7801 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7861 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7921 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7981 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8041 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8101 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8161 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8221 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8281 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8341 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     8401 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     8461 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8521 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8581 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     8641 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     8701 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     8761 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     8821 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     8881 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     8941 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9001 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9061 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9121 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9181 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     9241 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
     9301 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9361 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9421 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
     9481 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
     9541 ggtcttgaaa ggagtgggaa tt
//
