LOCUS       sequence-38591-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pCDFBB-eGFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(64..70,71..88,89..94))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    109..130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(205..223)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      219..266
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ctcgcccttg ctcaccatat ggatccagat ctcctccttc tactagacgc tcacaattcc
       61 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      121 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggtctag ataagttcag
      181 gatgaattcc ctaggctgct gccaccgctg agcaataact agcataaccc cttggggcct
      241 ctaaacgggt cttgaggggt tttttgctga aacctcaggc atttgagaag cacacggtca
      301 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
      361 gaccctgccc tgaaccgacg accgggtcat cgtggccgga tcttgcggcc cctcggcttg
      421 aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
      481 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
      541 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
      601 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
      661 acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta
      721 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
      781 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
      841 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
//
