LOCUS       sequence-385917-       10577 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10577)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1973
FEATURES             Location/Qualifiers
     source          1..10577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(37..233)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        complement(45..255)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      51..186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(486..530)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    715..948
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1441..1566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1613..1793
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1794..2020)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2048..2068)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2048..2066)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2087..2103)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2087..2103)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2100..2122)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2111..2127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2135..2141,2142..2159,2160..2165))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2180..2201
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2318..2335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2489..3077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2569..2588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3248..4039,4040..4108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3871..3890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4109..4213)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4239..4694)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4376..4397)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4588..4607
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4821..4843
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4835..4852
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4836..4852
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4862..4881
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4862..4880
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4901..5036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5076..5197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5087..5106)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5141..5160
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5275..5508
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    5580..6168
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5686..5697
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6095..6115
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     regulatory      6494..6503
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8504..9682)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8513..9451
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(8536..8556)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9820..9937
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(join(9980..10577,1..5))
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10066..10086)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     join(10563..10577,1..5)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gtcatggtaa gctccggtgg tacctctaga aagctttttg caaaagccta ggcctccaaa
       61 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
      121 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
      181 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagac gcatgctttg
      241 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
      301 atgcaatgca tgggtcccct cggggttggg aggtgggtct gaaacgataa tggtgaatat
      361 ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa acctaccaag
      421 cctcctacta tcattatgaa taattttata taccacagcc aatttgttat gttaaaccaa
      481 ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt ttcttgctgg
      541 ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc
      601 cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact ccaactagca
      661 ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat ccccaggagc
      721 tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg cttgatgccc
      781 cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa attgttctgc
      841 tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg
      901 cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat tcccactgct
      961 cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc taatggttca
     1021 atttttacta ctttatattt atataattca cttctccaat tgtccctcat atctcctcct
     1081 ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc
     1141 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata
     1201 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg
     1261 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac
     1321 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt
     1381 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact
     1441 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact
     1501 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg
     1561 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag
     1621 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     1681 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     1741 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccgtttatg
     1801 tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt ggttcgtcca
     1861 atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca ccttacttcc
     1921 accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta actcatcgtt
     1981 accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc agctccagct
     2041 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     2101 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     2161 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     2221 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     2281 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2341 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2401 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2461 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2521 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2581 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2641 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2701 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2761 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2821 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2881 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2941 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3001 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3061 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3121 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3181 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3241 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     3301 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     3361 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     3421 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     3481 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     3541 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     3601 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     3661 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     3721 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3781 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3841 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3901 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3961 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4021 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4081 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4141 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4201 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     4261 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     4321 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     4381 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     4441 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     4501 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     4561 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     4621 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     4681 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     4741 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     4801 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     4861 gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa aaagcctcct
     4921 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     4981 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     5041 ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg agtttggaca
     5101 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5161 tttatttgta accattataa gctgcaataa acaagttcct ctcactctct gatattcatt
     5221 tctttgcaag ttataaatac tgaataataa gatgacatga actactatac gtactgctag
     5281 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     5341 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     5401 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     5461 aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt cccccctttt
     5521 cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt aaagtcgacg
     5581 cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa gacgcggaag
     5641 aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga gggccgaagg
     5701 gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga gcagccaagg
     5761 aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca gccgagcccc
     5821 tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag ggagggggaa
     5881 agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag tgggggttgc
     5941 gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca actcctcata
     6001 aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac gggaagcaat
     6061 agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag caacatagtt
     6121 aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt cgacttaacg
     6181 cgtagactgg aacacaccca gatacgttcg tcctttccac aagatatata aagccaagta
     6241 cggactagtt cattactacg gggacgcgct gcgggacctg tccggcttgg cttccaagcc
     6301 gctaaccaac cgctggatgc tctgcagttc gctggtggcc gcctctttct ccgcgcagag
     6361 tttgggcgac aaggacatgg agctggagga gagcgtggag gagcggctgt tgagttcaga
     6421 gcccgagtcc actgccaccg aggccgggct ggcggccagg gcggcgactt tgccgtccag
     6481 gggccccgcg gccgccgcca tggagggcag ggcggtggtg agcagactgc tgccgtccgg
     6541 gaccggcacc gggatggagt aggggctgta gcgcagccgc gggcgcatgg tgttcagatt
     6601 gaggaagggg tggcggtgca ccgagctgga ggctgccgca gaggaggcgg ccgccgctgc
     6661 ggccatgtac gtgtaggggt aagggaacag gcttccgaaa ggggacatgg ccaggccctg
     6721 agaggccagg acgtgctgct ggaggtggaa gggcagggtg gccgcggacg ccccggacag
     6781 tccctgcgcc gcagcggcag aggccatggc cgtggaatcc aggcccgaga caccggtgga
     6841 ggccccagaa accgtggcca ggaggggacc catgccagcg gccgccatgc tggagaaggc
     6901 gccccccatg gcaaactggc tggggtgcag gaagagcggg tgcccgttga agaactgttg
     6961 gcccgccagg cccggggcga agccgaggcc aggcaggggg ccctgggcca ggtgcgcggc
     7021 ggccgcgtcc gtctgcaccg tgagcggcgc gaaggcctcc ttgcccggga gcgcgcgcgc
     7081 ctcttccacc ttggccggcg ctgtgccctc gcgaaccggg ctcctgcgct cctccgcgcc
     7141 caggccgcga gtgctggacg agatggtggc ggggctatgg cgtgagtcgg gcgacgcttt
     7201 gtccagccgc ccgctgtccc ggggccgctc agcagcgaaa aggtgagcct tgaccgcggg
     7261 gctgcccttg tcacggcagg gctcctccga cgtggtggtg gagatcttgg ccgcgtcgca
     7321 ggcctcgggg ccatgctcct ctttgctctc ggcctcggcg tcgctctcac cctcgctggg
     7381 acataaatct ttgaggttcg atgtccctac agtggaggcg gctggagaag aagcctgggc
     7441 gaagcagttg aaagctgctt gttcactgga ggactcatca gaggtcccat tctccttttt
     7501 gtgtctttca tcaaacaccc tcatggactg cagggtgagc tgttttcttt tttctcttcg
     7561 gccatttcca gtgtcccgga aaccttttgc aaaagggttg ttgtctattt ttaactgggt
     7621 tatcttatca ttctggtatg cagtcacagc gatgaattca gtttcgggga acaagtatgt
     7681 ccgaaatgta ctataaggga gtttcaagat gtcattggct cttacaatgt ggaaccgggg
     7741 ctggtatttg tgcatggagt tcaatatagt ctgagtacca aaactataat tcccctgcca
     7801 cgtagcgtga tcacttggga aggccaaagt aaatccatgt ttgtctgaaa tgttgttggt
     7861 gagtttcagt ttgtggaaag tgacgacttt ggacatccac tgttccccag tagcggggct
     7921 gtccgggtga atgtacatcc tctttggcat ttcggggtcg gccttaccag ccaccatcca
     7981 ccgagaattg tgaaatttat aacgacagtc atcagcagct ataatgtcca tcaataaaat
     8041 gtatttggct tttttatcca gcccagaaca tctcacttta aatggaggaa acattcgcct
     8101 tcccgacttg gtaatgacca tctcggtgcc ccgcttgtga aactgatccc aaagttcttt
     8161 agcctccagg tgcaccttgg ggtcgtcctc cacctcttct tcgggctcca tggtcttcaa
     8221 aggcctcaga tgcgcctggg gccccaggga ggagaacggg atgccggtct cggccgcccc
     8281 caccaattga tccatgatcg gcttggccag ggcgcccggc agcgagagcg ccgccgcgcc
     8341 gttgggaggc agcgtcagcg cggggaagaa cggcggctgg tgacccagca ccgcgctcat
     8401 ggcgaagtcc ggcgcccggt gaggtaggaa cggatggtag gccatgcttg tcccaggaat
     8461 gaccggatct ctcatggaga ggctcatggt ggcggtgcta gcctcacgac acctgaaatg
     8521 gaagaaaaaa actttgaacc actgtctgag gcttgagaat gaaccaagat ccaaactcaa
     8581 aaagggcaaa ttccaaggag aattacatca agtgccaagc tggcctaact tcagtctcca
     8641 cccactcagt gtggggaaac tccatcgcat aaaacccctc cccccaacct aaagacgacg
     8701 tactccaaaa gctcgagaac taatcgaggt gcctggacgg cgcccggtac tccgtggagt
     8761 cacatgaagc gacggctgag gacggaaagg cccttttcct ttgtgtgggt gactcacccg
     8821 cccgctctcc cgagcgccgc gtcctccatt ttgagctccc tgcagcaggg ccgggaagcg
     8881 gccatctttc cgctcacgca actggtgccg accgggccag ccttgccgcc cagggcgggg
     8941 cgatacacgg cggcgcgagg ccaggcacca gagcaggccg gccagcttga gactaccccc
     9001 gtccgattct cggtggccgc gctcgcaggc cccgcctcgc cgaacatgtg cgctgggacg
     9061 cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat accagtgtgc agatcttggc
     9121 ccgcatttac aagactatct tgccagaaaa aaagcgtcgc agcaggtcat caaaaatttt
     9181 aaatggctag agacttatcg aaagcagcga gacaggcgcg aaggtgccac cagattcgca
     9241 cgcggcggcc ccagcgccca ggccaggcct caactcaagc acgaggcgaa ggggctcctt
     9301 aagcgcaagg cctcgaactc tcccacccac ttccaacccg aagctcggga tcaagaatca
     9361 cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg gccataaccc gtaaagaggc
     9421 caggcccgcg ggaaccacac acggcactta cctgtgttct ggcggcaaac ccgttgcgaa
     9481 aaagaacgtt cacggcgact actgcactta tatacggttc tcccccaccc tcgggaaaaa
     9541 ggcggagcca gtacacgaca tcactttccc agtttacccc gcgccacctt ctctaggcac
     9601 cggttcaatt gccgacccct ccccccaact tctcggggac tgtgggcgat gtgcgctctg
     9661 cccactgacg ggcaccggag ccaattccca ctcctttcaa gacctagaag gtccattagc
     9721 tgcaaagatt cctctctgtt taaaacttta tccatctttg cacctgcagg cctcgagccg
     9781 gcgccaaagt ggatctctgc tgtccctgta ataaacccga aaattttgaa tttttgtaat
     9841 ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc
     9901 tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata ctgccatttg
     9961 tctcaagatc tagaattcgt taactggcac cgggcttgcg ggtcatgcac caggtgcgcg
    10021 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
    10081 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
    10141 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
    10201 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
    10261 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
    10321 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccacggcg gcgccgtcgt
    10381 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
    10441 cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg
    10501 cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca
    10561 ccgtgggctt gtactcg
//
