LOCUS       sequence-38593-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-ptet-eGFP
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(45..70,71..76,77..93,94..99,100..118))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    75..93
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      145..188
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(192..210)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        278..382
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(601..620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctcctcgccc ttgctcacca tatggatcca gatctcctcc ttctggtcag tgcgtcctgc
       61 tgatgtgctc agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggatc
      121 tagataagtt caggatgaat tccagaaatc atccttagcg aaagctaagg atttttttta
      181 tctgaaattc tgcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      241 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      361 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      421 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      481 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      541 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      601 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      661 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      721 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      781 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      841 gggggatcat gtaactcgcc ttgatcgttg gga
//
