LOCUS       sequence-385949-       10405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0273
FEATURES             Location/Qualifiers
     source          1..10405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3343..3931
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3396..3416)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3814..3825)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4003..4236
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4314..4435
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4351..4370)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4405..4424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4475..4610
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4630..4649)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4631..4649)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4659..4676)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4659..4675)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4668..4690)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4817..5272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4904..4923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5114..5135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5298..5402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5403..5471,5472..6263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5621..5640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6434..7022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6923..6942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7176..7193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7310..7331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7346..7351,7352..7369,7370..7376)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7384..7400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7389..7411
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7408..7424
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7408..7424
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7443..7463
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7445..7463
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7491..7717
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7718..7898
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7945..8070
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8563..8796
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8981..9025
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9256..9466
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9278..9474
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9325..9460
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9506..10108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9506..9525)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10002..10022
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10151..10268
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ccagccgcag aggatggcgc ctgtgggcac agacaaggag ctcagtgacc tcctggactt
     1261 cagcatgatg ttcccgctgc ctgtcaccaa cgggaagggc cggcccgcct ccctggccgg
     1321 ggcgcagttc ggaggttcag gtcttgagga ccggcccagc tcaggctcct ggggcagcgg
     1381 cgaccagagc agctcctcct ttgaccccag ccggaccttc agcgagggca cccacttcac
     1441 tgagtcgcac agcagcctct cttcatccac attcctggga ccgggactcg gaggtgagtg
     1501 cctggcctgg tgcggtccct ctgctgtgca cagatgtgca gacgtgggcc ttggcatggt
     1561 cagtgcacgc acagcgccag gcaagagcgg tgagcggggc gcctatgcct ccttcgggag
     1621 agacgcaggc gtgggcggcc tgactcaggc tggcttcctg tcaggcgagc tggccctcaa
     1681 cagccccggg cccctgtccc cttcgggcat gaaggggacc tcccagtact acccctccta
     1741 ctccggcagc tcccggcgga gagcggcaga cggcagccta gacacgcagc ccaagaaggt
     1801 ccggaaggtc ccgccgggtc ttccatcctc ggtgtaccca cccagctcag gtgaggacta
     1861 cggcagggat gccaccgcct acccgtccgc caagaccccc agcagcacct atcccgcccc
     1921 cttctacgtg gcagatggca gcctgcaccc ctcagccgag ctctggagtc ccccgggcca
     1981 ggcgggcttc gggcccatgc tgggtggggg ctcatccccg ctgcccctcc cgcccggtag
     2041 cggcccggtg ggcagcagtg gaagcagcag cacgtttggt ggcctgcacc agcacgagcg
     2101 tatgggctac cagctgcatg gagcagaggt gaacggtggg ctcccatctg catcctcctt
     2161 ctcctcagcc cccggagcca cgtacggcgg cgtctccagc cacacgccgc ctgtcagcgg
     2221 ggccgacagc ctcctgggct cccgagggac cacagctggc agctccgggg atgccctcgg
     2281 caaagcactg gcctcgatct actccccgga tcactcaagc aataacttct cgtccagccc
     2341 ttctaccccc gtgggctccc cccagggcct ggcaggaacg tcacagtggc ctcgagcagg
     2401 agcccccggt gccttatcgc ccagctacga cgggggtctc cacggcctgc agagtaagat
     2461 agaagaccac ctggacgagg ccatccacgt gctccgcagc cacgccgtgg gcacagccgg
     2521 cgacatgcac acgctgctgc ctggccacgg ggcgctggcc tcaggtttca ccggccccat
     2581 gtcactgggc gggcggcacg caggcctggt tggaggcagc caccccgagg acggcctcgc
     2641 aggcagcacc agcctcatgc acaaccacgc ggccctcccc agccagccag gcaccctccc
     2701 tgacctgtct cggcctcccg actcctacag tgggctaggg cgagcaggtg ccacggcggc
     2761 cgccagcgag atcaagcggg aggagaagga ggacgaggag aacacgtcag cggctgacca
     2821 ctcggaggag gagaagaagg agctgaaggc cccccgggcc cggaccagta cggacgaggt
     2881 gctgtccctg gaggagaaag acctgaggga ccgggagagg cgcatggcca ataacgcgcg
     2941 ggagcgggtg cgcgtgcggg atattaacga ggccttccgg gagctggggc gcatgtgcca
     3001 gatgcacctc aagtcggaca aagcgcagac caagctgctc atcctgcagc aggccgtgca
     3061 ggtcatcctg gggctggagc agcaggtgcg agagcggaac ctgaatccca aagcagcctg
     3121 tttgaaacgg cgagaagagg aaaaggtgtc aggtgtggtt ggagaccccc agatggtgct
     3181 ttcagctccc cacccaggcc tgagcgaagc ccacaacccc gccgggcaca tgtagtaatg
     3241 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     3301 cgaatattgt ttcgtctgcg tgaggatacg cgttaagtcg acaatcaacc tctggattac
     3361 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3421 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3481 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     3541 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     3601 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     3661 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     3721 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     3781 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     3841 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3901 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     3961 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     4021 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     4081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     4141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     4201 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     4261 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     4321 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4381 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4441 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4501 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4561 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     4621 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     4681 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4741 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4801 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4861 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4921 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4981 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5041 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5101 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5161 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5221 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5281 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5341 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5401 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5461 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5521 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5581 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5641 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5701 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5761 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5821 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5881 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5941 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6001 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6061 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6121 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6181 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6241 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6301 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6361 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6421 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6481 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6541 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6601 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6661 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6721 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6781 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6841 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6901 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6961 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7021 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7081 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7141 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7201 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7261 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7321 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7381 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7441 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     7501 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     7561 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     7621 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     7681 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     7741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     7921 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     7981 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     8041 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     8101 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     8161 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     8221 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     8281 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     8341 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     8401 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     8461 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     8521 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     8581 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     8641 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     8701 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     8761 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     8821 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     8881 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     8941 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     9001 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     9061 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     9121 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     9181 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
     9241 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
     9301 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     9361 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     9421 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     9481 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     9541 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     9601 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     9661 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     9721 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     9781 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     9841 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     9901 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     9961 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    10021 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    10081 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    10141 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    10201 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    10261 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    10321 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    10381 ctaggtcttg aaaggagtgg gaatt
//
