LOCUS       sequence-385951-       10342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0274
FEATURES             Location/Qualifiers
     source          1..10342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3280..3868
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3333..3353)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3751..3762)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3940..4173
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4251..4372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4288..4307)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4342..4361
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4412..4547
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4567..4586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4568..4586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4596..4613)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4596..4612)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4605..4627)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4754..5209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4841..4860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5051..5072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5235..5339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5340..5408,5409..6200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5558..5577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6371..6959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6860..6879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7113..7130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7247..7268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7283..7288,7289..7306,7307..7313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7321..7337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7326..7348
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7345..7361
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7345..7361
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7380..7400
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7382..7400
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7428..7654
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7655..7835
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7882..8007
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8500..8733
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8918..8962
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9193..9403
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9215..9411
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9262..9397
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9443..10045
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9443..9462)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9939..9959
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10088..10205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ccagccgcag aggatggcgc ctgtgggcac agacaaggag ctcagtgacc tcctggactt
     1261 cagcatgatg ttcccgctgc ctgtcaccaa cgggaagggc cggcccgcct ccctggccgg
     1321 ggcgcagttc ggaggttcag gcaagagcgg tgagcggggc gcctatgcct ccttcgggag
     1381 agacgcaggc gtgggcggcc tgactcaggc tggcttcctg tcaggcgagc tggccctcaa
     1441 cagccccggg cccctgtccc cttcgggcat gaaggggacc tcccagtact acccctccta
     1501 ctccggcagc tcccggcgga gagcggcaga cggcagccta gacacgcagc ccaagaaggt
     1561 ccggaaggtc ccgccgggtc ttccatcctc ggtgtaccca cccagctcag gtgaggacta
     1621 cggcagggat gccaccgcct acccgtccgc caagaccccc agcagcacct atcccgcccc
     1681 cttctacgtg gcagatggca gcctgcaccc ctcagccgag ctctggagtc ccccgggcca
     1741 ggcgggcttc gggcccatgc tgggtggggg ctcatccccg ctgcccctcc cgcccggtag
     1801 cggcccggtg ggcagcagtg gaagcagcag cacgtttggt ggcctgcacc agcacgagcg
     1861 tatgggctac cagctgcatg gagcagaggt gaacggtggg ctcccatctg catcctcctt
     1921 ctcctcagcc cccggagcca cgtacggcgg cgtctccagc cacacgccgc ctgtcagcgg
     1981 ggccgacagc ctcctgggct cccgagggac cacagctggc agctccgggg atgccctcgg
     2041 caaagcactg gcctcgatct actccccgga tcactcaagc aataacttct cgtccagccc
     2101 ttctaccccc gtgggctccc cccagggcct ggcaggaacg tcacagtggc ctcgagcagg
     2161 agcccccggt gccttatcgc ccagctacga cgggggtctc cacggcctgc agagtaagat
     2221 agaagaccac ctggacgagg ccatccacgt gctccgcagc cacgccgtgg gcacagccgg
     2281 cgacatgcac acgctgctgc ctggccacgg ggcgctggcc tcaggtttca ccggccccat
     2341 gtcactgggc gggcggcacg caggcctggt tggaggcagc caccccgagg acggcctcgc
     2401 aggcagcacc agcctcatgc acaaccacgc ggccctcccc agccagccag gcaccctccc
     2461 tgacctgtct cggcctcccg actcctacag tgggctaggg cgagcaggtg ccacggcggc
     2521 cgccagcgag atcaagcggg aggagaagga ggacgaggag aacacgtcag cggctgacca
     2581 ctcggaggag gagaagaagg agctgaaggc cccccgggcc cggaccaggt gccaacccac
     2641 ccctcggcac tccccaccct caccccatca ggacgcacat gtgcacaggc cacacgcaca
     2701 ccgcacccac actgggaggc cgtcggcggg ccccacgctc ttcccccaac ctcactgtct
     2761 ccctctggcc ccctcccggc gccctcccca tagcccagac gaggacgagg acgaccttct
     2821 ccccccagag cagaaggccg agcgggagaa ggagcgccgg gtggccaata acgcccggga
     2881 gcggctgcgg gtccgtgaca tcaacgaggc ctttaaggag ctggggcgca tgtgccaact
     2941 gcacctcaac agcgagaagc cccagaccaa actgctcatc ctgcaccagg ctgtctcggt
     3001 catcctgaac ttggagcagc aagtgcgaga gcggaacctg aatcccaaag cagcctgttt
     3061 gaaacggcga gaagaggaaa aggtgtcagg tgtggttgga gacccccaga tggtgctttc
     3121 agctccccac ccaggcctga gcgaagccca caaccccgcc gggcacatgt agtaatgaac
     3181 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     3241 cgcgagccta cttcctgata gtgcacgcgt taagtcgaca atcaacctct ggattacaaa
     3301 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     3361 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     3421 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3481 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3541 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3601 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3661 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     3721 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3781 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3841 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     3901 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     3961 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     4021 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4081 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4141 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     4201 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     4261 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4321 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     4381 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4441 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4501 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     4561 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     4621 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4681 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4741 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4801 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4861 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4921 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4981 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     5041 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     5101 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     5161 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     5221 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     5281 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     5341 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     5401 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     5461 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     5521 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     5581 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5641 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5701 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5761 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5821 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5881 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5941 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6001 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6061 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6121 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6181 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     6241 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     6301 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     6361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     6421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     6481 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     6541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7081 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7141 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     7201 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     7261 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     7321 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     7381 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     7441 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     7501 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     7561 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     7621 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7681 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7741 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7801 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7861 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7921 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7981 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     8041 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     8101 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     8161 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     8221 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     8281 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     8341 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8401 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8461 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8521 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8581 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8641 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8701 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8761 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8821 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8881 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8941 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     9001 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     9061 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     9121 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
     9181 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
     9241 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9301 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9361 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
     9421 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     9481 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     9541 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     9601 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
     9661 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     9721 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     9781 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     9841 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     9901 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     9961 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    10021 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    10081 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    10141 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    10201 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    10261 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    10321 ggtcttgaaa ggagtgggaa tt
//
