LOCUS       sequence-385952-       10330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10330)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0275
FEATURES             Location/Qualifiers
     source          1..10330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3268..3856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3321..3341)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3739..3750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3928..4161
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4239..4360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4276..4295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4330..4349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4400..4535
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4555..4574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4556..4574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4584..4601)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4584..4600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4593..4615)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4742..5197
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4829..4848)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5039..5060
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5223..5327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5328..5396,5397..6188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5546..5565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6359..6947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6848..6867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7101..7118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7235..7256
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7271..7276,7277..7294,7295..7301)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7309..7325
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7314..7336
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7333..7349
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7333..7349
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7368..7388
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7370..7388
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7416..7642
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7643..7823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7870..7995
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8488..8721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8906..8950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9181..9391
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9203..9399
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9250..9385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9431..10033
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9431..9450)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9927..9947
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10076..10193
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 ccagccgcag aggatggcgc ctgtgggcac agacaaggag ctcagtgacc tcctggactt
     1261 cagcatgatg ttcccgctgc ctgtcaccaa cgggaagggc cggcccgcct ccctggccgg
     1321 ggcgcagttc ggaggttcag gtcttgagga ccggcccagc tcaggctcct ggggcagcgg
     1381 cgaccagagc agctcctcct ttgaccccag ccggaccttc agcgagggca cccacttcac
     1441 tgagtcgcac agcagcctct cttcatccac attcctggga ccgggactcg gaggcaagag
     1501 cggtgagcgg ggcgcctatg cctccttcgg gagagacgca ggcgtgggcg gcctgactca
     1561 ggctggcttc ctgtcaggcg agctggccct caacagcccc gggcccctgt ccccttcggg
     1621 catgaagggg acctcccagt actacccctc ctactccggc agctcccggc ggagagcggc
     1681 agacggcagc ctagacacgc agcccaagaa ggtccggaag gtcccgccgg gtcttccatc
     1741 ctcggtgtac ccacccagct caggtgagga ctacggcagg gatgccaccg cctacccgtc
     1801 cgccaagacc cccagcagca cctatcccgc ccccttctac gtggcagatg gcagcctgca
     1861 cccctcagcc gagctctgga gtcccccggg ccaggcgggc ttcgggccca tgctgggtgg
     1921 gggctcatcc ccgctgcccc tcccgcccgg tagcggcccg gtgggcagca gtggaagcag
     1981 cagcacgttt ggtggcctgc accagcacga gcgtatgggc taccagctgc atggagcaga
     2041 ggtgaacggt gggctcccat ctgcatcctc cttctcctca gcccccggag ccacgtacgg
     2101 cggcgtctcc agccacacgc cgcctgtcag cggggccgac agcctcctgg gctcccgagg
     2161 gaccacagct ggcagctccg gggatgccct cggcaaagca ctggcctcga tctactcccc
     2221 ggatcactca agcaataact tctcgtccag cccttctacc cccgtgggct ccccccaggg
     2281 cctggcagga acgtcacagt ggcctcgagc aggagccccc ggtgccttat cgcccagcta
     2341 cgacgggggt ctccacggcc tgcagagtaa gatagaagac cacctggacg aggccatcca
     2401 cgtgctccgc agccacgccg tgggcacagc cggcgacatg cacacgctgc tgcctggcca
     2461 cggggcgctg gcctcaggtt tcaccggccc catgtcactg ggcgggcggc acgcaggcct
     2521 ggttggaggc agccaccccg aggacggcct cgcaggcagc accagcctca tgcacaacca
     2581 cgcggccctc cccagccagc caggcaccct ccctgacctg tctcggcctc ccgactccta
     2641 cagtgggcta gggcgagcag gtgccacggc ggccgccagc gagatcaagc gggaggagaa
     2701 ggaggacgag gagaacacgt cagcggctga ccactcggag gaggagaaga aggagctgaa
     2761 ggccccccgg gcccggacca gcccagacga ggacgaggac gaccttctcc ccccagagca
     2821 gaaggccgag cgggagaagg agcgccgggt ggccaataac gcccgggagc ggctgcgggt
     2881 ccgtgacatc aacgaggcct ttaaggagct ggggcgcatg tgccaactgc acctcaacag
     2941 cgagaagccc cagaccaaac tgctcatcct gcaccaggct gtctcggtca tcctgaactt
     3001 ggagcagcaa gtgcgagagc ggaacctgaa tcccaaagca gcctgtttga aacggcgaga
     3061 agaggaaaag gtgtcaggtg tggttggaga cccccagatg gtgctttcag ctccccaccc
     3121 aggcctgagc gaagcccaca accccgccgg gcacatgtag taatgaacta gtccgtaact
     3181 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgatt tctcggagag
     3241 gcggccgagc caacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     3301 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3361 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3421 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     3481 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     3541 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     3601 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     3661 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     3721 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     3781 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     3841 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     3901 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3961 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4021 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4081 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4141 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     4201 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     4261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     4381 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     4441 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     4501 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     4561 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     4621 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4681 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4741 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4801 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4861 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4921 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4981 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5041 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     5101 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5161 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     5221 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5281 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5341 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5401 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5461 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5521 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5581 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5641 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5701 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5761 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5821 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5881 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5941 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     6001 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     6061 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     6121 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     6181 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6241 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6481 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6601 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6661 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6721 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6781 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6841 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6901 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6961 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     7021 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     7081 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     7141 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     7201 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7261 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7321 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     7381 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     7441 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     7501 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     7561 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     7621 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7801 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7861 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7921 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7981 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     8041 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     8101 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     8161 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     8221 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     8281 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     8341 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     8401 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     8461 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     8521 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     8581 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     8641 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8701 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8761 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8821 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8881 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8941 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     9001 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     9061 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     9121 acccatgcat tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     9181 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
     9241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9301 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9361 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
     9421 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     9481 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     9541 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     9601 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac
     9661 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     9721 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     9781 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     9841 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     9901 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     9961 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
    10021 cggtgccagt taacgaattc tagatcttga gacaaatggc agtattcatc cacaatttta
    10081 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
    10141 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
    10201 attacaggga cagcagagat ccactttggc gccggctcga ggcctgcagg tgcaaagatg
    10261 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
    10321 agtgggaatt
//
