LOCUS       sequence-385959-        9609 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Third generation lentiviral vector expressing Kinesin Heavy Chain 5 
            isoform B fused to Myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9609)
  AUTHORS   Zamponi E, Meehl JB, Voeltz GK
  TITLE     The ER ladder is a unique morphological feature of developing 
            mammalian axons.
  JOURNAL   Dev Cell. 2022 Jun 6;57(11):1369-1382.e6. doi: 
            10.1016/j.devcel.2022.05.002. Epub 2022 May 23.
  PUBMED    35609616
REFERENCE   2  (bases 1 to 9609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2022.05.002"; journalName: "Dev Cell"; date: 
            "2022-06-6- 6"; volume: "57"; issue: "11"; pages: "1369-1382.e6"
COMMENT     Sequence Label: pLV-Kif5b-Myc
FEATURES             Location/Qualifiers
     source          1..9609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    81..102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(117..122,123..140,141..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    155..171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     160..182
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     179..195
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     179..195
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     214..234
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        216..234
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        262..488
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             489..669
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    716..841
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1334..1567
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1752..1796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2179
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2202..2505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2506..2709
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2660..2680
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2706..2730
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5675..5704
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    5714..6302
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5767..5787)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6185..6196)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6389..6622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6694..6815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6731..6750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6785..6804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6855..6990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7010..7029)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7011..7029)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7039..7056)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7039..7055)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7048..7070)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7197..7652
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7284..7303)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7494..7515
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7678..7782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7783..7851,7852..8643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8001..8020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8814..9402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9303..9322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9556..9573
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
       61 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggctttc
      121 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      181 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
      241 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
      301 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
      361 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
      421 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
      481 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
      541 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
      601 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
      661 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
      721 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
      781 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
      841 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
      901 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
      961 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     1021 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     1081 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     1141 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     1201 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     1261 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     1321 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     1381 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     1441 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     1501 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     1561 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     1621 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     1681 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     1741 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     1801 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     1861 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     1921 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     1981 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     2041 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tatagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaatt caaaatttta tcgataagct tgggagttcc gcgttacata acttacggta
     2221 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2281 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2341 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2401 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2461 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2521 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     2581 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     2641 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     2701 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2761 ctccatagaa gacaccgact ctagaatggc ggacctggcc gagtgcaaca tcaaagtgat
     2821 gtgtcgcttc agacctctca acgagtctga agtgaaccgc ggcgacaagt acatcgccaa
     2881 gtttcaggga gaagacacgg tcgtgatcgc gtccaagcct tatgcatttg atcgggtgtt
     2941 ccagtcaagc acatctcaag agcaagtgta taatgactgt gcaaagaaga ttgttaaaga
     3001 tgtacttgaa ggatataatg gaacaatatt tgcatatgga caaacatcct ctgggaagac
     3061 acacacaatg gagggtaaac ttcatgatcc agaaggcatg ggaattattc caagaatagt
     3121 gcaagatatt tttaattata tttactccat ggatgaaaat ttggaatttc atattaaggt
     3181 ttcatatttt gaaatatatt tggataagat aagggacctg ttagatgttt caaagaccaa
     3241 cctttcagtt catgaagaca aaaaccgagt tccctatgta aaggggtgca cagagcgttt
     3301 tgtatgtagt ccagatgaag ttatggatac catagatgaa ggaaaatcca acagacatgt
     3361 agcagttaca aatatgaatg aacatagctc taggagtcac agtatatttc ttattaatgt
     3421 caaacaagag aacacacaaa cggaacaaaa gctgagtgga aaactttatc tggttgattt
     3481 agctggtagt gaaaaggtta gtaaaactgg agctgaaggt gctgtgctgg atgaagctaa
     3541 aaacatcaac aagtcacttt ctgctcttgg aaatgttatt tctgctttgg ctgagggtag
     3601 tacatatgtt ccatatcgag atagtaaaat gacaagaatc cttcaagatt cattaggtgg
     3661 caactgtaga accactattg taatttgctg ctctccatca tcatacaatg agtctgaaac
     3721 aaaatctaca ctcttatttg gccaaagggc caaaacaatt aagaacacag tttgtgtcaa
     3781 tgtggagtta actgcagaac agtggaaaaa gaagtatgaa aaagaaaaag aaaaaaataa
     3841 gatcctgcgg aacactattc agtggcttga aaatgagctc aacagatggc gtaatgggga
     3901 gacggtgcct attgatgaac agtttgacaa agagaaagcc aacttggaag ctttcacagt
     3961 ggataaagat attactctta ccaatgataa accagcaacc gcaattggag ttataggaaa
     4021 ttttactgat gctgaaagaa gaaagtgtga agaagaaatt gctaaattat acaaacagct
     4081 tgatgacaag gatgaagaaa ttaaccagca aagtcaactg gtagagaaac tgaagacgca
     4141 aatgttggat caggaggagc ttttggcatc taccagaagg gatcaagaca atatgcaagc
     4201 tgagctgaat cgccttcaag cagaaaatga tgcctctaaa gaagaagtga aagaagtttt
     4261 acaggcccta gaagaacttg ctgtcaatta tgatcagaag tctcaggaag ttgaagacaa
     4321 aactaaggaa tatgaattgc ttagtgatga attgaatcag aaatcggcaa ctttagcgag
     4381 tatagatgct gagcttcaga aacttaagga aatgaccaac caccagaaaa aacgagcagc
     4441 tgagatgatg gcatctttac taaaagacct tgcagaaata ggaattgctg tgggaaataa
     4501 tgatgtaaag cagcctgagg gaactggcat gatagatgaa gagttcactg ttgcaagact
     4561 ctacattagc aaaatgaagt cagaagtaaa aaccatggtg aaacgttgca agcagttaga
     4621 aagcacacaa actgagagca acaaaaaaat ggaagaaaat gaaaaggagt tagcagcatg
     4681 tcagcttcgt atctctcaac atgaagccaa aatcaagtca ttgactgaat accttcaaaa
     4741 tgtggaacaa aagaaaagac agttggagga atctgtcgat gccctcagtg aagaactagt
     4801 ccagcttcga gcacaagaga aagtccatga aatggaaaag gagcacttaa ataaggttca
     4861 gactgcaaat gaagttaagc aagctgttga acagcagatc cagagccata gagaaactca
     4921 tcaaaaacag atcagtagtt tgagagatga agtagaagca aaagcaaaac ttattactga
     4981 tcttcaagac caaaaccaga aaatgatgtt agagcaggaa cgtctaagag tagaacatga
     5041 gaagttgaaa gccacagatc aggaaaagag cagaaaacta catgaactta cggttatgca
     5101 agatagacga gaacaagcaa gacaagactt gaagggtttg gaagagacag tggcaaaaga
     5161 acttcagact ttacacaacc tgcgcaaact ctttgttcag gacctggcta caagagttaa
     5221 aaagagtgct gagattgatt ctgatgacac cggaggcagc gctgctcaga agcaaaaaat
     5281 ctcctttctt gaaaataatc ttgaacagct cactaaagtg cacaaacagt tggtacgtga
     5341 taatgcagat ctccgctgtg aacttcctaa gttggaaaag cgacttcgag ctacagctga
     5401 gagagtgaaa gctttggaat cagcactgaa agaagctaaa gaaaatgcat ctcgtgatcg
     5461 caaacgctat cagcaagaag tagatcgcat aaaggaagca gtcaggtcaa agaatatggc
     5521 cagaagaggg cattctgcac agattgctaa acctattcgt cccgggcaac atccagcagc
     5581 ttctccaact cacccaagtg caattcgtgg aggaggtgca tttgttcaga acagccagcc
     5641 agtggcagtg cgaggtggag gaggcaaaca agtggaacaa aaacttattt ctgaagaaga
     5701 tctgtaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5761 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5821 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5881 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5941 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6001 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6061 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     6121 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6181 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6241 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6301 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6361 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6421 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6481 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6541 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6601 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     6661 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     6721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6781 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     6841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6961 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7021 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7081 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7141 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7201 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7261 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7321 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     7381 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     7441 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     7501 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     7561 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     7621 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     7681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     8341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     8401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     8461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     8521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     8581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     9001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     9061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     9121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     9181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     9241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     9301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     9541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9601 ccgcctctc
//
