LOCUS       sequence-38596-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-eGFP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(65..71,72..89,90..95))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      193..236
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(240..258)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        326..430
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(649..668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctcgccctt gctcaccata tggatccaga tctcctcctt ctactagacg ctcacaattc
       61 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggtcta gataagttca
      181 ggatgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg
      241 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      301 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      361 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      421 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      481 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      541 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      601 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      661 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      721 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      781 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      841 gacaacgatc ggaggaccga aggagctaac cgctttttgc
//
