LOCUS       sequence-385962-        6421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BDLF3.5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BDLF3.5
FEATURES             Location/Qualifiers
     source          1..6421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..116
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    132..231
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1489..1588)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1606..1624)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1629..1645)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2076..2867
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2960..3548
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3449..3468
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3702..3719
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3878..3905)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3997..4083)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4147..4163
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4179..4278
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5523..6092
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             5523..6092
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(6091..6190)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6208..6226)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6231..6247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
       61 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
      121 agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
      181 ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagttggc atggtcgatg
      241 aacaagtggc ggtggaacac ggcaccgtca gccacacaat cagccgggag gaagatggcg
      301 tggtccacga gcggcgtgtc ttggccagcg gggagagggt ggaagtcttt tataaggctc
      361 cggccccacg acctcgggag ggacgggcct ctaccttcca cgacttcacc gtcccggcgg
      421 ctgctgccgt cccagggccg gaacctgagc ctgagccaca cccacctatg ccaatccatg
      481 ccaatggggg aggagagacc aagaccaata cccaggatca gaatcaaaat cagaccaccc
      541 ggacccggac caatgccaaa gctgaagaac ggactgcgga gatggatgac accatggcct
      601 cgtcaggagg gcagagaggg gcaccaattt ccgcggacct actctctctc tcctcgttaa
      661 ctggtagaat ggcagccatg gccccctcct ggatgaagag cgaggtatgc ggtgagagaa
      721 tgagattcaa ggaagatgtc tatgatggag aggcagagac cctagctgag cctccacgct
      781 gtttcatgct gagctttgtt ttcatctatt actgctgcta tttggcgttc ctggccctac
      841 tcgcctttgg ttttaaccca ctctttttgc ccagctttat gccggtgggg gccaaggtgc
      901 ttcggggtaa ggggcgtgat tttggggtgc ccctgtctta tgggtgtccg accaatccat
      961 tctgcaaggt ttacaccctt atcccggccg tggtcattaa caatgtgact tattacccca
     1021 acaacacgga cagccatggg ggtcatggtg gatttgaggc ggctgccctt catgtagctg
     1081 cactttttga gtctgggtgc ccaaatctac aggctgtgac taataggaac aggacattta
     1141 acgtcaccag agccagtggc cgagttgaaa ggcgccttgt acaagatatg cagagggtcc
     1201 tggcgagtgc tgtggtggtg atgcatcatc actgccacta tgagacatat tatgtctttg
     1261 atggggtggg ccccgagttt ggtaccattc ctacgccctg cttcaaggat gtgttggcct
     1321 ttaggccgtc attggtgacc aactgcaccg cgccgttaaa gacatccgtc aagggtccta
     1381 actggtcagg ggcagctgga ggcatgaaac ggaagcaatg tcgtgttgac cggctcacgg
     1441 accgctcatt ccctgcatac ctcgaggagg tcatgtatgt gatggttcag ccaactttct
     1501 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1561 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1621 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1681 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     1741 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1801 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1861 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1921 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1981 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     2041 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2101 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2161 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2221 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2281 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2341 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2401 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2461 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2521 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2581 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2641 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2701 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2761 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2821 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2881 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2941 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3001 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3061 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3121 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3181 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3241 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3301 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3361 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3421 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3481 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3541 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3601 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3661 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3721 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3781 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3841 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3901 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3961 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     4021 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4081 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     4141 gacgttgtaa aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac
     4201 tgatagtgac ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt
     4261 tgtacaaaaa agttggcatg aggcctaaaa aggatggctt ggaagacttt ctgaggctaa
     4321 ctcctgaaat caaaaagcag ctgggctctc tggtctctga ctactgcaac gtcctcaaca
     4381 aggaatttac agccgggagt gtggagatta ctctgagatc ctacaaaata tgcaaggcat
     4441 ttataaatga ggccaaggcc cacgggcgag aatggggcgg gctaatggcc acgctcaaca
     4501 tctgcaattt ttgggccatt ctccgaaaca acagggtaag aagacgggct gagaatgccg
     4561 gcaacgacgc atgttccatc gcgtgcccta tagtgatgcg ctacgtgtta gaccacctga
     4621 tagtggtcac tgacagattc ttcatccagg cccccagcaa ccgggtgatg attcctgcca
     4681 ccataggcac cgctatgtac aagctcctaa aacacagtcg ggtgcgggcc tacacctaca
     4741 gcaaggtgct gggcgtggac cgcgcggcca tcatggcctc cggcaagcag gtagtggaac
     4801 acctgaacag gatggagaag gaaggcctcc taagctccaa gttcaaggcc ttttgcaagt
     4861 gggtgttcac ctatcccgtc ctcgaggaga tgttccagac tatggtctcg tccaagacag
     4921 gccatctgac ggacgatgtt aaggatgtca gggctctgat taagacactg ccccgggcct
     4981 cctactccag ccacgccgga cagaggagct acgtgagcgg cgtgcttccc gcgtgcctgc
     5041 tgtcaaccaa gtccaaggca gtggaaactc ctatcctcgt gtccggagcc gacaggatgg
     5101 acgaggagct catggggaat gatgggggtg cctctcacac cgaggcccgc tactcggagt
     5161 ccggacagtt tcatgctttt acagatgaac tcgaaagtct cccgagcccg accatgcccc
     5221 tgaagcccgg tgcccaaagc gccgactgcg gtgacagcag ttccagcagc agtgactcgg
     5281 gcaacagtga caccgagcag agcgagcggg aagaggccag ggccgaggcc ccgcgcctgc
     5341 gggccccaaa gtcgcgccgg acatccaggc ccaaccgtgg tcaaactcca tgtccttcca
     5401 acgcggcgga acctgaacag ccttggatag cagcggtcca ccaagagagc gatgagagac
     5461 ccatattccc ccacccctca aagcccacct ttcttcctcc cgttaaaagg aagaagggcc
     5521 tcagggatag ccgggaaggt atgttcctgc caaagccgga agcgggcagt gccatatctg
     5581 acgtgttcga ggggcgagag gtgtgtcagc caaagaggat caggcccttc catccacccg
     5641 gatccccgtg ggccaaccgg cccctgcctg cctctttggc tcccaccccc acaggacctg
     5701 tccatgaacc ggtcggatcc ctaacgccag ccccggtgcc ccagccactt gacccggccc
     5761 ccgcagtaac ccccgaggca agtcatctgt tggaggaccc tgatgaagaa accagtcagg
     5821 ccgtgaaggc cctaagggag atggctgaca ctgttattcc ccagaaggag gaagcagcca
     5881 tatgtggaca gatggacctg agccacccgc cccctcgtgg ccatttggac gaactgacca
     5941 caacactaga gtccatgaca gaggatttga atctggactc ccccctgacc cccgaactta
     6001 atgaaatctt ggatacattt ctaaatgatg aatgtctgct gcatgccatg catatttcaa
     6061 ctgggctgtc tatttttgac accagcttat ttccaacttt cttgtacaaa gttggcatta
     6121 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa
     6181 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg
     6241 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa
     6301 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac
     6361 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat
     6421 a
//
