LOCUS       sequence-385977-        3693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BaRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BaRF1(RNR-s)
FEATURES             Location/Qualifiers
     source          1..3693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1572..1671)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1688..1707)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1689..1707)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1712..1728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1712..1728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2159..2950
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3043..3631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3532..3551
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tccaagttgt tgtacgtgcg tgatcatgag ggctttgcct gcctaacggt
      721 cgaaacccac cgcaaccgct ggttcgcggc tcacattgtc ctcaccaagg actgcgggtg
      781 tctcaagcta ctcaatgaga gggacttgga gttttacaag ttcctcttta cgttcctggc
      841 catggccgag aagcttgtga actttaacat tgatgaactg gtcaccagct tcgagagcca
      901 cgacattgat cactactaca ccgagcagaa ggccatggag aacgtccacg gggagactta
      961 tgctaacatt ttaaacatgc tctttgatgg ggacagggcg gcgatgaacg cctacgcaga
     1021 ggccatcatg gccgacgagg ccctgcaagc caagatttcc tggctccgtg acaaggtggc
     1081 ggccgccgtc accctgccgg agaagattct tgtgttcctg ctgattgaag gcatcttctt
     1141 cattagctcc ttctacagca tagccctgct gcgggtccgg ggcctaatgc ctggcatctg
     1201 cctggccaat aactacataa gtagggatga gctgctccac acccgcgctg cctccctgtt
     1261 atacaatagc atgacagcca aggctgaccg accaagggcc acctggatcc aggagctgtt
     1321 tcgcactgcg gtggaggtag agactgcctt catcgaggct cgtggagagg gggttacctt
     1381 ggtggatgtg cgagccataa agcagtttct ggaggccacg gccgatcgca tcctgggtga
     1441 cattggtcag gctcccttgt atggcacacc accccccaag gactgcccgc tcacctacat
     1501 gactagcatc aagcaaacta atttctttga gcaagagagt tccgattaca ccatgctggt
     1561 ggtagatgac cttccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     1621 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     1681 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     1741 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgcctcct
     1801 ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg
     1861 cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     1921 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     1981 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     2041 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     2101 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacattat
     2161 gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     2221 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     2281 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     2341 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     2401 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     2461 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     2521 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     2581 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     2641 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     2701 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     2761 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     2821 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     2881 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     2941 cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt
     3001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     3121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     3181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     3241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     3301 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     3361 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     3421 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     3481 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     3541 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     3601 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     3661 cctggccttt tgctggcctt ttgctcacat gtt
//
