LOCUS       sequence-385982-        4747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses right-side TALE–DddAtox C term half cox1-FusXTBE in 
            zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Sabharwal A, Kar B, Restrepo-Castillo S, Holmberg SR, Mathew ND, 
            Kendall BL, Cotter RP, WareJoncas Z, Seiler C, Nakamaru-Ogiso E, 
            Clark KJ, Ekker SC
  TITLE     The FusX TALE Base Editor (FusXTBE) for Rapid Mitochondrial DNA 
            Programming of Human Cells In Vitro and Zebrafish Disease Models In 
            Vivo.
  JOURNAL   CRISPR J. 2021 Dec;4(6):799-821. doi: 10.1089/crispr.2021.0061. Epub
            2021 Nov 30.
  PUBMED    34847747
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/crispr.2021.0061"; journalName: "CRISPR J"; date: 
            "2021-12"; volume: "4"; issue: "6"; pages: "799-821"
COMMENT     Sequence Label: Cox1-FusXTBE-RightTALE
FEATURES             Location/Qualifiers
     source          1..4747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..21
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     5'UTR           30..72
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             2424..2675
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     3'UTR           2723..2847
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
     primer_bind     complement(2984..3003)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2995..3804
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3062..3081)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3947..4535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4436..4455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4689..4706
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgaattaacc ctcactaaag ggaagcttgc ttgttctttt tgcagaagct cagaataaac
       61 gctcaacttt ggcagatctc accatggcag gttatttgaa ggtcctaagc tctctcagca
      121 ggtctgctgc cactctatcc aaaagccccg ccgttcttgc tccagcttgc cggagtttgc
      181 aacagaggaa ctatgccgat aaacgtatcc agtctaggga cgtcactaga gtggatctac
      241 gcacgctcgg ctacagtcag cagcagcaag agaagatcaa accgaaggtg cgttcgacag
      301 tggcgcagca ccacgaggca ctggtgggcc atgggtttac acacgcgcac atcgttgcgc
      361 tcagccaaca cccggcagcg ttagggaccg tcgctgtcac gtatcagcac ataatcacgg
      421 cgttgccaga ggcgacacac gaagacatcg ttggcgtcgg caaacagtgg tccggcgcac
      481 gcgccctgga ggccttgctc acggatgcgg gggagttgag aggtccgccg ttacagttgg
      541 acacaggcca acttgtgaag attgcaaaac gtggcggcgt gaccgcaatg gaggcagtgc
      601 atgcatcgcg caatgcactg acgggtgccc ccctgaacct gaccccggac caagtggtgg
      661 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
      721 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
      781 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
      841 gcctgacccc ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg
      901 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
      961 tggtggctat cgccagcaac ggtggcggca agcaagcgct cgaaacggtg cagcggctgt
     1021 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     1081 acaatggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     1141 accatggcct gaccccggac caagtggtgg ctatcgccag caacggtggc ggcaagcaag
     1201 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     1261 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     1321 ggctgttgcc ggtgctgtgc caggaccatg gcctcacacc cgatcaggtc gtggctatcg
     1381 ccagcaacaa tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     1441 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac attggcggca
     1501 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     1561 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     1621 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     1681 ctatcgccag caacattggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     1741 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
     1801 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     1861 gcctgacccc ggaccaagtg gtggctatcg ccagcaacat tggcggcaag caagcgctcg
     1921 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag
     1981 tggtggctat cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt
     2041 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     2101 acattggcgg caagcaagcg ctcgaaagca ttgtggccca gctgagccgg cctgatccgg
     2161 cgttggccgc gttgaccaac gaccacctcg tcgccttggc ctgcctcggc ggacgtcctg
     2221 ccatggatgc agtgaaaaag ggattgccgc acgcgccgga attgatcaga agagtcaatc
     2281 gccgtattgg cgaacgcacg tcccatcgcg ttgcctctag agccattcca gtgaagcgcg
     2341 gcgctaccgg tgaaaccaaa gtgtttaccg gtaacagcaa cagcccgaag agcccgacca
     2401 aaggcggttg ctctggcggc tcaactaatc tgagcgacat cattgagaag gagactggga
     2461 aacagctggt cattcaggag tccatcctga tgctgcctga ggaggtggag gaagtgatcg
     2521 gcaacaagcc agagtctgac atcctggtgc acaccgccta cgacgagtcc acagatgaga
     2581 atgtgatgct gctgacctct gacgcccccg agtataagcc ttgggccctg gtcatccagg
     2641 attctaacgg cgagaataag atcaagatgc tgtgataggc actagttagt gactgactag
     2701 gatctggcta gcgttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct
     2761 acataatacc aacttacact tacaaaatgt tgtcccccaa aatgtagcca ttcgtatctg
     2821 ctcctaataa aaagaaagtt tcttcacatt ctaaaaaaaa aaaaaaaaaa aaaaaaaaac
     2881 cccccccccc cccgcatgcc tgcaggtcga ctaggatcac tagtgcggcc gcccgcggct
     2941 gcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     3001 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     3061 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     3121 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     3181 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     3241 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     3301 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     3361 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     3421 cgcgtatttc gcctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3481 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     3541 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3601 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3661 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3721 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3781 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcaaaaa
     3841 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3901 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3961 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     4021 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     4081 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     4141 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     4201 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4261 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4321 gatacctaca gcgtgagcta tgagaaagcg ccacggttcc cgaagggaga aaggcggaca
     4381 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4441 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4501 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4561 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4621 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4681 ccgagcgcag cgagtcagtg agcgaggaag cggaacagat cgacgataga gcgcccaata
     4741 cgcaaac
//
