LOCUS       sequence-385988-       12575 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination vector to express C-terminal TurboID fusion 
            under UAS promoter, for making transgenic flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12575)
  TITLE     Drosophila gateway destination vectors to express TurboID fusion 
            proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pWALIUM10-GW-TurboID-V5
FEATURES             Location/Qualifiers
     source          1..12575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(33..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1453..2067,2138..2269,2473..2788,2850..3504,
                     3579..3852,4254..4325))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     4777..4795
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    4918..4987
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5225..5655
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5673..5706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5719..5813
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5829..5862
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5875..5969
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5993..6231
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6178..6199
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    6277..6401
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6426..6431,6432..6449,6450..6456)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6510..7169
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6576..6595)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7511..7816
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     7695..7714
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(7857..7981)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             8016..8972
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8979..9020
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          9311..9376
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9506..9526
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9798..9919
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9835..9854)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9889..9908
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9941..10371
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     primer_bind     complement(10390..10410)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10390..10408)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10429..10445)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10429..10445)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10442..10464)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10453..10469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10477..10483,10484..10501,10502..10507))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10522..10543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10660..10677)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10831..11419)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10911..10930)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11590..12381,12382..12450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12451..12555)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
       61 ctcatttttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcrm ktyccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 caagcttcac gacctaggcg cgccctagtt ccagtgaaat ccaagcattt tctaaattaa
      721 atgtattctt attattatag ttgttatttt tgatatatat aaacaacact attatgccca
      781 ccattttttt gagatgcatc tacacaagga acaaacactg gatgtcactt tcagttcaaa
      841 ttgtaacgct aatcactccg aacaggtcac aaaaaattac cttaaaaagt cataatatta
      901 aattagaata aatatagctg tgagggaaat atatacaaat atattggagc aaataaattg
      961 tacatacaaa tatttattac taatttctat tgagacgaaa tgaaccactc ggaaccattt
     1021 gagcgaaccg aatcgcgcgg aactaacgac agtcgctcca aggtcgtcga acaaaaggtg
     1081 aatgtgttgc ggagagcggg tgggagacag cgaaagagca actacgaaac gtggtgtggt
     1141 ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat gtatataaaa aatatatccc
     1201 ggtgttttat gtagcgataa acgagttttt gatgtaaggt atgcaggtgt gtaagtcttt
     1261 tggttagaag acaaatccaa agtctacttg tggggatgtt cgaaggggaa atacttgtat
     1321 tctataggtc atatcttgtt tttattggca caaatataat tacattagct ttttgagggg
     1381 gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa aaaaacaagc agttatttcg
     1441 gatatatgtc ggctactcct tgcgtcgggc ccgaagtctt agagccagat atgcgagcac
     1501 ccggaagctc acgatgagaa tggccagacc cacgtagtcc agcggcagat cggcggcgga
     1561 gaagttaagc gtctccagga tgaccttgcc cgaactgggg cacgtggtgt tcgacgatgt
     1621 gcagctaatt tcgcccggct ccacgtccgc ccattggtta atcagcagac cctcgttggc
     1681 gtaacggaac catgagaggt acgacaacca tttgaggtat actggcaccg agcccgagtt
     1741 caagaagaag ccgccaaaga gcaggaatgg tatgataacc ggcggaccca cagacagcgc
     1801 catcgaggtc gaggagctgg cgcaggatat tagatatccg aaggacgttg acacattggc
     1861 caccagagtg accagcgcca ggcagttgaa gaagtgcagc actccggccc gcagtccgat
     1921 catcggatag gcaatcgccg tgaagaccag tggcactgtg agaaaaagcg gcaattcggc
     1981 aatcgttttg cccagaaagt atgtgtcaca gcgataaagt cgacttcggg cctccctcat
     2041 aaaaactggc agctctgagg tgaacaccta aatcgaatcg attcattaga aagttagtaa
     2101 attattgaaa tgcaaatgta ttctaaacat gacttacatt tatcgtggca aagacgtttt
     2161 gaaaggtcat gttggtcagg aagaggaaga tggctccgtt gatattcatc acacccactt
     2221 gcgtgagttg ttggcccaaa aagatgaggc caatcaagat ggcaaccatc tgcaaattaa
     2281 aatgttactc gcatctcatt aatattcgcg agttaaatga aatttattta tcttctgcaa
     2341 aactataaac tatacatctc attgaaaaaa actaagaagg gtgtggaatc aggcaattct
     2401 atctaaaatc tagcgaattt gtttccaaga attgtaagcg ttatatcatt tgtttccact
     2461 ggaaccactc accgttgtct gaataagtcg cacttttacg aggagtggtt ccttgagcac
     2521 cgacagccag gatcgccaca ggaccgcccg gaactgcatg aaccaggtgg ccttgtaggt
     2581 gtacccattc tccggctgct ccagtggctt ctccagattt ttggtggcca acaactgctc
     2641 catatcccgg gctactttgc taatggcaaa attgtcgcat atcttggcga tccgatcacg
     2701 ggactcgatc tcccgtccgg gcacaacggc caacacctgt acgtaaaagt ccgccggatt
     2761 gtagttggta ggacactggg cacccacgct ggataggagt tgagatgtta tgtaatacta
     2821 gataccctta ataaacacat cgaactcact aggaaaagaa gtcgacggct tcgctgggag
     2881 tgcccaagaa agctaccctg ccctcggcca tcagaaggat cttgtcaaag agctcaaaca
     2941 gctcggaaga cggctgatga atggtcagga tgacggtctt gcccttctgc gacagcttct
     3001 tcagcacctg gacgacgctg tgggcggtaa aggagtccag tccggaggtg ggctcatcgc
     3061 agatcagaag cggcggatcg gttagagcct cggaggcgaa tgccagacgc ttcctttctc
     3121 cgccggacag acctttcacc ctgccgggca caccgatgat cgtgtgctga catttgctga
     3181 gcgaaagctc ctggatcacc tgatccacgc gggccactcg ctgccgatag gtcagatgtc
     3241 gtggcatccg caccatggct tggaaaatca ggtgttccct ggccgttagg gagccgataa
     3301 agaggtcatc ctgctggaca taggcgcacc tggcctgcat ctccttggcg tccacaggtt
     3361 ggccattgag cagtcgcatc ccggatggcg atacttggat gccctgcggc gatcgaaagg
     3421 caagggcatt cagcagggtc gtctttccgg caccggaact gcccatcacg gccaaaagtt
     3481 cgcccggata ggccacgccg caaactgagt ttcaaattgg taattggacc ctttattaag
     3541 atttcacaca gatcagccga ctgcgaatag aaactcaccg ttcttgagca aatgtttcct
     3601 gggcgccggt atgtgtcgct cgttgcagaa tagtccgcgt gtccggttga ccagctgccg
     3661 ccatccggag cccggctgat tgaccgcccc aaagatgtcc atattgtgcc aggcataggt
     3721 gaggttctcg gctagttggc cgctccctga accggagtcc tccggcggac tgggtggccg
     3781 gagcgtgccg tagtttttgg cctgcccgaa gccctggtta atgcagctct gcgaagccgc
     3841 tccgctgtca ccctgcaatg ataggggatc tcaaatatca actacaagcg ttatgctcat
     3901 ctaaccccga acaaaaagta ccccgaagta tcctacgaag taggtttata cttttattta
     3961 ttttttgtgc atctaggatc agcttaaaat atctggttgt tatatttttt gtaaaaaaga
     4021 atatagtcga aaatgaatgc ctttagatgt cttgatcatg atatgatctc aaaaattgtc
     4081 ttatatagcg agaacagcta ccagaataat ctgtttcgtg tcactatttg tttgtgcaat
     4141 tgcggtttgg gatttttgtg ggtcgcagtt ctcacgccgc agacaatttg atgttgcaat
     4201 cgcagttcct atagatcaag tgaacttaag atgtatgcac atgtactact cacattgttc
     4261 agatgctcgg cagatgggtg tttgctgcct ccgcgaatta atagctcctg atcctcttgg
     4321 cccattgccg ggatttttca cactttcccc tgcttaccca cccaaaacca atcaccaccc
     4381 caatcactca aaaaacaaac aaaaataaga agcgagagga gttttggcac agcactttgt
     4441 gtttaattga tggcgtaaac cgcttggagc ttcgtcacga aaccgctgac aaaatgcaac
     4501 tgaaggcgga cattgacgct acgtaacgct acaaacggtg gcgaaagaga tagcggacgc
     4561 agcggcgaaa gagacggcga tatttctgtg gacagagaag gaggcaaaca gcgctgactt
     4621 tgagtggaat gtcattttga gtgagaggta atcgaaagaa cctggtacat caaataccct
     4681 tggatcgaag taaatttaaa actgatcaga taagttcaat gatatccagt gcagtaaaaa
     4741 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
     4801 ctaggcgcgc cagatcgcaa gaagcttgat atcatcgatc tcgaggctgc atccaacgcg
     4861 ttgggagctc tccggatcaa ttcggcttca ggtaccgtcg acgatgtagg tcacggtctc
     4921 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
     4981 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
     5041 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
     5101 gggacgtgcg acggcgtcgg ctggtgcgga tacgcggggc agcgtcagcg ggttctcgac
     5161 ggtcacggcg ggcatgtcga caagccgaat tgatccacta gaaggcctaa ttcggtacac
     5221 tagttggcca cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg
     5281 caaagtaaaa ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa
     5341 accaaataat tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta
     5401 ataaaaaata ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta
     5461 ttatattgca tacccgtttt taataaaata cattgcatac cctcttttaa taaagaatat
     5521 tgcatacgtt gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat
     5581 accttttctt gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt
     5641 gtgccaacca acaacactag tagtaccagc ttataacttc gtataatgta tgctatacga
     5701 agttatctgc aggcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5761 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5821 tcccatggat aacttcgtat aatgtatgct atacgaagtt atggatccgc aggtcggagt
     5881 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     5941 gtcctccgag cggagtactg tcctccgagc ggagactcgt cgacagcgag cgccggagta
     6001 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     6061 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     6121 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     6181 actactgaaa tctgccaaga agtaattatt gaatacaaga agagaactct gaatagggaa
     6241 ttgggaattc gcagatctgc ggccgcggct cgaatcacaa gtttgtacaa aaaagctgaa
     6301 cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag
     6361 actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc
     6421 caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt
     6481 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     6541 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     6601 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     6661 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     6721 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     6781 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     6841 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     6901 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     6961 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     7021 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     7081 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     7141 actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta aaagccagat
     7201 aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata
     7261 cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag
     7321 cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca
     7381 accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa
     7441 gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg
     7501 ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     7561 tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc
     7621 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     7681 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     7741 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     7801 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
     7861 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
     7921 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
     7981 tgattcgagg gtacctcagc ggtggcagcg gcggtaaaga caatactgtg cctctgaagc
     8041 tgatcgctct cctggctaat ggcgagttcc atagtggcga acagctggga gaaaccctgg
     8101 gcatgtccag ggccgctatc aacaagcaca ttcagactct gcgcgactgg ggcgtggacg
     8161 tgttcaccgt gcccggaaag ggctactctc tgcccgagcc tatcccgctg ctgaacgcta
     8221 aacagattct gggacagctg gacggcggga gcgtggcagt cctgcctgtg gtcgactcca
     8281 ccaatcagta cctgctggat cgaatcggcg agctgaagag tggggatgct tgcattgcag
     8341 aatatcagca ggcagggaga ggaagcagag ggaggaaatg gttctctcct tttggagcta
     8401 acctgtacct gagtatgttt tggcgcctga agcggggacc agcagcaatc ggcctgggcc
     8461 cggtcatcgg aattgtcatg gcagaagcgc tgcgaaagct gggagcagac aaggtgcgag
     8521 tcaaatggcc caatgacctg tatctgcagg atagaaagct ggcaggcatc ctggtggagc
     8581 tggccggaat aacaggcgat gctgcacaga tcgtcattgg cgccgggatt aacgtggcta
     8641 tgaggcgcgt ggaggaaagc gtggtcaatc agggctggat cacactgcag gaagcaggga
     8701 ttaacctgga caggaatact ctggccgcta cgctgatccg agagctgcgg gcagccctgg
     8761 aactgttcga gcaggaaggc ctggctccat atctgccacg gtgggagaag ctggataact
     8821 tcatcaatag acccgtgaag ctgatcattg gggacaaaga gattttcggg attagccggg
     8881 ggattgataa acagggagcc ctgctgctgg aacaggacgg agttatcaaa ccctggatgg
     8941 gcggagaaat cagtctgcgg tctgccgaaa aggctagcgg caagcccatc cccaaccccc
     9001 tgctgggcct ggacagcacc tagagcaaac tagttctgat ctgctagaca attgttggca
     9061 tcaggtaggc atcacacacg attaacaacc cctaaaaata cactttgaaa atattgaaaa
     9121 tatgtttttg tatacatttt tgatattttc aaataatacg cagttataaa actcattagc
     9181 taacccattt tttctttgct tatgcttaca gattgcaaag aactagagcc gcgggatctt
     9241 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     9301 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     9361 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     9421 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     9481 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     9541 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     9601 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     9661 aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag
     9721 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     9781 atggaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9841 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9901 aactcatcaa tgtatcttat catgtctggt tccagagctc tggccacgta ataagtgtgc
     9961 gttgaattta ttcgcaaaaa cattgcatat tttcggcaaa gtaaaatttt gttgcatacc
    10021 ttatcaaaaa ataagtgctg catacttttt agagaaacca aataattttt tattgcatac
    10081 ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc atactttgac
    10141 gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc cgtttttaat
    10201 aaaatacatt gcataccctc ttttaataaa gaatattgca tacgttgacg aaacaaattt
    10261 tcgttgcata cccaataaaa gattattata ttgcatacct tttcttgcca taccatttag
    10321 ccgatcaatt gtgctcggca acagtatatt tgtggtgtgc caaccaacaa cgagctccag
    10381 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
    10441 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    10501 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    10561 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10621 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
    10681 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    10741 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    10801 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    10861 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10921 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10981 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    11041 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    11101 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    11161 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    11221 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    11281 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    11341 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    11401 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    11461 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    11521 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    11581 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    11641 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    11701 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    11761 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    11821 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    11881 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11941 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    12001 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    12061 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    12121 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    12181 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    12241 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    12301 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    12361 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    12421 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    12481 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    12541 aataggggtt ccgcgcacat ttccccgaaa agtgc
//
