LOCUS       sequence-38599-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-lacZa
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(94..100,101..118,119..124))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    139..160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      222..265
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(269..287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        355..459
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(678..697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ctctagagtc gacctgcagg catgcaagct tggcgtaatc atggtcatat ggatccagat
       61 ctcctccttc tactagacgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      181 cgctttccag tcgggtctag ataagttcag gatgaattcc agaaatcatc cttagcgaaa
      241 gctaaggatt ttttttatct gaaattctgc ctcgtgatac gcctattttt ataggttaat
      301 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      661 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      841 agtgataaca ctgcggccaa cttacttctg acaacgatcg
//
