LOCUS       sequence-385996-        9226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            COMP5AP-AviTag-9xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-LTF-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3065..3100
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3110..3133
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4793..4837
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             4838..4864
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5004..5021)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5010..5121
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5239..5259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5244..5573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5424..5559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5486..5505
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5621..5668
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5687..6286
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5687..5706)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6183..6203
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6416..6537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6453..6472)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6507..6526
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6590..7381,7382..7450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7213..7232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7451..7542)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7584..7603
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7609..7628
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7714..8302
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8203..8222
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8456..8473
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8571..8588
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8572..8588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9044..9060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccggacgtag aagatctgtt cagtggtgcg ccgtgtctca
     1021 gcctgaggcc accaagtgtt tccagtggca gcggaacatg cggaaagtgc ggggacctcc
     1081 tgtgtcctgc atcaagaggg atagccccat ccagtgcatc caggccattg ccgagaacag
     1141 agccgatgcc gtgacactgg acggcggctt tatctatgaa gccggactgg ccccttacaa
     1201 gctgaggcct gttgccgccg aagtgtacgg cacagagaga cagccccgga cacactatta
     1261 tgccgtggcc gtggttaaga aaggcggcag cttccagctg aacgagctgc agggactgaa
     1321 gtcctgtcac accggcctga gaagaaccgc cggatggaat gtgcccatcg gaaccctgag
     1381 gcctttcctg aattggacag gccctcctga gcctatcgaa gccgctgtgg ccagattctt
     1441 cagcgcctct tgtgtgcctg gcgccgacaa gggccagttt cctaatctgt gcagactgtg
     1501 cgccggcacc ggcgagaaca aatgtgcctt cagcagccaa gagccttact tcagctacag
     1561 cggcgccttc aagtgcctga gagatggcgc tggggatgtc gccttcatca gagaaagcac
     1621 cgtgttcgag gacctgagcg acgaggccga gagagatgag tacgagctgc tgtgccccga
     1681 caacaccaga aagcccgtgg acaagttcaa ggactgccac ctggccagag tgccttctca
     1741 tgctgtggtg gccagaagcg tgaacggcaa agaggacgcc atctggaacc tgctgagaca
     1801 ggcccaagag aagttcggca aggacaagag ccccaagttc cagctgtttg gctctcccag
     1861 cggccagaag gacctgctgt ttaaggatag cgccatcggc ttcagccggg tgccacctag
     1921 aattgacagc ggcctgtatc tcggctccgg ctactttacc gccatccaga acctgcggaa
     1981 gtccgaggaa gaagtggctg ccagaagggc cagagttgtt tggtgcgctg tgggcgagca
     2041 agagctgaga aagtgcaatc agtggtccgg cctgtctgag ggcagcgtga catgtagctc
     2101 tgccagcacc accgaggact gtattgccct ggtgctgaag ggcgaagccg acgccatgtc
     2161 tctcgatggc ggatacgtgt acaccgccgg caaatgtgga ctggtgcctg tgctggccga
     2221 gaactacaag agccagcaga gcagcgaccc cgatcctaac tgtgtggaca gacccgtgga
     2281 aggctatctg gccgtggctg ttgtgcggag aagcgataca agcctgacct ggaactccgt
     2341 gaagggcaag aagtcttgcc acaccgccgt ggacagaaca gctggctgga atatccctat
     2401 gggcctgctg ttcaaccaga ccggcagctg caagttcgac gagtacttca gccagtcttg
     2461 cgcccctggc agcgatccta gaagcaatct gtgcgccctg tgcatcggag atgagcaggg
     2521 cgaaaacaaa tgcgtgccca acagcaacga gcggtactac ggatacaccg gggccttcag
     2581 atgcctggcc gaaaatgccg gcgacgtggc ctttgtgaag gacgtgacag tgctgcagaa
     2641 caccgacggc aacaacaatg aggcctgggc caaagacctg aagctggccg attttgccct
     2701 gctgtgtctg gacggcaagc ggaagcctgt gacagaggcc agatcttgtc acctggctat
     2761 ggcccctaat cacgccgtgg tgtccagaat ggacaaggtg gaacggctga aacaggtgct
     2821 gctgcaccag caggccaagt ttggcagaaa cggcagcgac tgccccgata agttctgcct
     2881 gttccagagc gagacaaaga acctcctgtt caacgacaat accgagtgtc tggcccggct
     2941 gcacggcaag acaacctacg agaagtatct gggccctcag tacgtggccg gcatcaccaa
     3001 tctgaagaag tgcagcacaa gccctctgct ggaagcctgc gagttcctga gaaaaggcgc
     3061 gccggaagat caagttgatc caagattgat tgatggcaag ggcggatctc tggaggtgct
     3121 gttccaggga ccaggcggaa gcggcaatcc cggcggcgat ctggcccccc agatgctgcg
     3181 cgagctgcag gagaccaatg ccgccctgca ggacgtgcgc gagctgctgc gccaccgcgt
     3241 gaaggagatc accttcctga agaacaccgt gatggagtgc gacgcctgcg gtatgcagcc
     3301 ctctagaggc tccggctcca tcatcccagt tgaggaggag aacccggact tctggaaccg
     3361 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     3421 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     3481 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     3541 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     3601 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     3661 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     3721 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     3781 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     3841 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     3901 ggacattgac gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga
     3961 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     4021 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat
     4081 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat
     4141 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     4201 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     4261 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     4321 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     4381 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     4441 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     4501 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     4561 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     4621 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     4681 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     4741 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtggcg gaggactgaa
     4801 cgacatcttc gaggctcaga aaatcgaatg gcacgaacat caccatcacc atcaccatca
     4861 ccattaaggc cggcctgtag tcactggcac aacgcgttac ccggtaagcc aatcgggtat
     4921 acacggtcgt catactgcag acagggttct tctactttgc aagatagtct tgagtagtaa
     4981 aataaataga tagagaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5041 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5101 ataaaatgag gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta
     5161 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5221 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5281 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5341 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5401 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5461 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     5521 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     5581 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     5641 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa
     5701 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5761 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     5821 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     5881 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     5941 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6001 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6061 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6121 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc
     6181 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6241 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga
     6301 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6361 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6421 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6481 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6541 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct
     6601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
     6721 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
     6781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6901 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7441 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
     7561 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
     7621 accccttgct catgaccaaa atcccttaac gtgagttacg cgcgcgtcgt tccactgagc
     7681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7861 tcttctagtg tagccgtagt tagcccacca cttcaagaac tctgtagcac cgcctacata
     7921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8461 gtcagtgagc gaggaagcgg aaggcgagag tagggaactg ccaggcatca aactaagcag
     8521 aaggcccctg acggatggcc tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga
     8581 cggccagtct taagctcggg ccccctgggc ggttctgata acgagtaatc gttaatccgc
     8641 aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag ggaaagagca
     8701 tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat atatgagaat tatttaacct
     8761 tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa
     8821 cacctataat taaactattc atctattatt tatgattttt tgtatataca atatttctag
     8881 tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg
     8941 atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat
     9001 cagtatttat tatcatttag aataaatttt gtgtcgccct taattgtgag cggataacaa
     9061 ttacgagctt catgcacagt ggcgttgact caatattggc cattagccat attattcatt
     9121 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     9181 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
