LOCUS       sequence-385997-        9331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9331)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-TEK-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3170..3205
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3215..3238
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4898..4942
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             4943..4969
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5109..5126)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5115..5226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5344..5364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5349..5678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5529..5664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5591..5610
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5726..5773
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5792..6391
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5792..5811)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6288..6308
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6521..6642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6558..6577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6612..6631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6695..7486,7487..7555))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7318..7337
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7556..7647)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7689..7708
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7714..7733
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7819..8407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8308..8327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8561..8578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8676..8693
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8677..8693
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9149..9165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgcaatgga cctgatcctg atcaatagcc tgcctctggt
     1021 gtccgacgcc gagacatctc tgacatgtat tgcctctgga tggcggcctc acgagcctat
     1081 caccatcgga agagatttcg aggccctgat gaaccagcat caggaccctc tggaagtgac
     1141 ccaggacgtg accagagagt gggccaagaa agtcgtgtgg aagagagaga aggccagcaa
     1201 gatcaacggc gcctactttt gcgagggcag agtgcgggga gaagccatca gaatccggac
     1261 catgaagatg cggcagcagg cctcttttct gcccgccaca ctgaccatga ccgtggacaa
     1321 gggcgacaac gtgaacatca gcttcaagaa ggtgctgatc aaagaagagg acgccgtcat
     1381 ctacaagaac ggcagcttca tccacagcgt gcccagacac gaagtgcccg acatcctgga
     1441 agtgcatctg cctcatgctc agcctcagga tgctggcgtg tactccgcca gatatatcgg
     1501 aggcaacctg ttcaccagcg ccttcacaag actgatcgtg cggagatgcg aggcccagaa
     1561 atggggccct gagtgcaatc acctgtgcac cgcctgtatg aacaacggcg tgtgtcacga
     1621 ggataccggc gagtgtatct gccctcctgg ctttatgggc agaacctgcg agaaggcctg
     1681 cgagctgcac accttcggca ggacctgtaa agagagatgt agcggccaag agggctgcaa
     1741 gagctacgtg ttctgcctgc ctgatcctta cggctgtagc tgtgccacag gatggaaggg
     1801 cctgcagtgc aatgaagcct gccatccagg cttctacggc cccgattgca agctgagatg
     1861 ctcctgcaac aatggcgaga tgtgcgacag attccagggc tgtctgtgtt ctcctggctg
     1921 gcagggactg cagtgtgaaa gagaaggcat ccagcggatg acccctaaga tcgtggatct
     1981 gcccgatcac atcgaagtga acagcggcaa gttcaacccc atctgcaaag ccagtggctg
     2041 gcccctgcct accaatgagg aaatgaccct ggtcaagccc gatggcacag tgctgcaccc
     2101 caaggacttc aaccacaccg atcacttcag cgtggccatc ttcaccatcc acagaatcct
     2161 gcctccagac tctggcgtgt gggtctgctc tgtgaatacc gtggccggca tggtggaaaa
     2221 gcccttcaac atcagcgtga aagtgctgcc caagcctctg aacgccccta acgtgatcga
     2281 caccggccac aacttcgccg tgatcaatat cagcagcgag ccctactttg gcgacggccc
     2341 catcaagagc aagaagctgc tgtacaagcc cgtgaaccac tacgaggcct ggcagcacat
     2401 ccaagtgacc aacgagatcg tgaccctgaa ctacctggaa cctcggaccg agtacgaact
     2461 gtgcgtgcag cttgttagaa gaggcgaagg cggcgaagga caccctggac ctgtcagaag
     2521 attcaccaca gcctctatcg gcctgccacc tcctagagga ctgaacctgc tgcctaagag
     2581 ccagacaacc ctgaacctga catggcagcc tatcttccct agcagcgagg acgatttcta
     2641 cgtggaagtg gaacggcgga gcgtgcagaa gtccgaccag cagaatatca aggtgcccgg
     2701 caatctgacc agcgtgctgc tgaacaatct gcaccctcgg gaacagtatg tcgtgcgggc
     2761 cagagtgaac acaaaagccc aaggcgagtg gtccgaggat ctgacagctt ggaccctgag
     2821 cgatattctc ccacctcagc ctgagaacat caagatcagc aacatcaccc acagcagcgc
     2881 cgtgattagc tggacaatcc tggacggcta cagcatcagc tccatcacca tcagatacaa
     2941 ggtgcagggc aagaacgagg accagcacgt ggacgtgaag atcaagaacg ccaccatcac
     3001 acagtaccag ctgaaaggcc tggaaccaga gacagcctac caggtggaca tcttcgccga
     3061 gaacaacatc ggcagcagca accctgcctt tagccacgag ctggtcacac tgcctgaatc
     3121 tcaggctcct gcagatctcg gcggaggcaa gatgcttctt ggcgcgccgg aagatcaagt
     3181 tgatccaaga ttgattgatg gcaagggcgg atctctggag gtgctgttcc agggaccagg
     3241 cggaagcggc aatcccggcg gcgatctggc cccccagatg ctgcgcgagc tgcaggagac
     3301 caatgccgcc ctgcaggacg tgcgcgagct gctgcgccac cgcgtgaagg agatcacctt
     3361 cctgaagaac accgtgatgg agtgcgacgc ctgcggtatg cagccctcta gaggctccgg
     3421 ctccatcatc ccagttgagg aggagaaccc ggacttctgg aaccgcgagg cagccgaggc
     3481 cctgggtgcc gccaagaagc tgcagcctgc acagacagcc gccaagaacc tcatcatctt
     3541 cctgggcgat gggatggggg tgtctacggt gacagctgcc aggatcctaa aagggcagaa
     3601 gaaggacaaa ctggggcctg agatacccct ggccatggac cgcttcccat atgtggctct
     3661 gtccaagaca tacaatgtag acaaacatgt gccagacagt ggagccacag ccacggccta
     3721 cctgtgcggg gtcaagggca acttccagac cattggcttg agtgcagccg cccgctttaa
     3781 ccagtgcaac acgacacgcg gcaacgaggt catctccgtg atgaatcggg ccaagaaagc
     3841 agggaagtca gtgggagtgg taaccaccac acgagtgcag cacgcctcgc cagccggcac
     3901 ctacgcccac acggtgaacc gcaactggta ctcggacgcc gacgtgcctg cctcggcccg
     3961 ccaggagggg tgccaggaca tcgctacgca gctcatctcc aacatggaca ttgacgtgat
     4021 cctaggtgga ggccgaaagt acatgtttcg catgggaacc ccagaccctg agtacccaga
     4081 tgactacagc caaggtggga ccaggctgga cgggaagaat ctggtgcagg aatggctggc
     4141 gaagcgccag ggtgcccggt atgtgtggaa ccgcactgag ctcatgcagg cttccctgga
     4201 cccgtctgtg acccatctca tgggtctctt tgagcctgga gacatgaaat acgagatcca
     4261 ccgagactcc acactggacc cctccctgat ggagatgaca gaggctgccc tgcgcctgct
     4321 gagcaggaac ccccgcggct tcttcctctt cgtggagggt ggtcgcatcg accatggtca
     4381 tcatgaaagc agggcttacc gggcactgac tgagacgatc atgttcgacg acgccattga
     4441 gagggcgggc cagctcacca gcgaggagga cacgctgagc ctcgtcactg ccgaccactc
     4501 ccacgtcttc tccttcggag gctaccccct gcgagggagc tccatcttcg ggctggcccc
     4561 tggcaaggcc cgggacagga aggcctacac ggtcctccta tacggaaacg gtccaggcta
     4621 tgtgctcaag gacggcgccc ggccggatgt taccgagagc gagagcggga gccccgagta
     4681 tcggcagcag tcagcagtgc ccctggacga agagacccac gcaggcgagg acgtggcggt
     4741 gttcgcgcgc ggcccgcagg cgcacctggt tcacggcgtg caggagcaga ccttcatagc
     4801 gcacgtcatg gccttcgccg cctgcctgga gccctacacc gcctgcgacc tggcgccccc
     4861 cgccggcacc accgacgccg cgcacccggg tggcggagga ctgaacgaca tcttcgaggc
     4921 tcagaaaatc gaatggcacg aacatcacca tcaccatcac catcaccatt aaggccggcc
     4981 tgtagtcact ggcacaacgc gttacccggt aagccaatcg ggtatacacg gtcgtcatac
     5041 tgcagacagg gttcttctac tttgcaagat agtcttgagt agtaaaataa atagatagag
     5101 aaaatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5161 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5221 tgcatcacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     5281 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     5341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     5401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     5461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     5521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5581 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     5641 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     5701 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     5761 agtataatac gacaaggtga ggaactaaac catgaccgag tacaagccca cggtgcgcct
     5821 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     5881 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     5941 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6001 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6061 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6121 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6181 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6241 ggccgagcgc gccggggtgc ccgccttcct ggagacatcc gcgccccgca acctcccctt
     6301 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6361 ctggtgcatg acccgcaagc ccggtgcctg acacgtgcta cgagatttcg attccaccgc
     6421 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6481 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     6601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     6661 gacctctagc tagagcttgg cgtaatcatg gtcattacca atgcttaatc agtgaggcac
     6721 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6781 taactacgat acgggagggc ttaccatctg gccccagcgc tgcgatgata ccgcgagaac
     6841 cacgctcacc ggctccggat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6901 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     6961 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccatcgct acaggcatcg
     7021 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7081 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7141 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7201 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7261 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7321 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7381 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7441 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7501 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatattct
     7561 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7621 ttgaatgtat ttagaaaaat aaacaaatag gggtcagtgt tacaaccaat taaccaattc
     7681 tgaacattat cgcgagccca tttatacctg aatatggctc ataacacccc ttgctcatga
     7741 ccaaaatccc ttaacgtgag ttacgcgcgc gtcgttccac tgagcgtcag accccgtaga
     7801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     7921 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     7981 gtagttagcc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8401 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8461 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8521 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8581 agcggaaggc gagagtaggg aactgccagg catcaaacta agcagaaggc ccctgacgga
     8641 tggccttttt gcgtttctac aaactctttc tgtgttgtaa aacgacggcc agtcttaagc
     8701 tcgggccccc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
     8761 ccgcttcggc gggttttttt atggggggag tttagggaaa gagcatttgt cagaatattt
     8821 aagggcgcct gtcactttgc ttgatatatg agaattattt aaccttataa atgagaaaaa
     8881 agcaacgcac tttaaataag atacgttgct ttttcgattg atgaacacct ataattaaac
     8941 tattcatcta ttatttatga ttttttgtat atacaatatt tctagtttgt taaagagaat
     9001 taagaaaata aatctcgaaa ataataaagg gaaaatcagt ttttgatatc aaaattatac
     9061 atgtcaacga taatacaaaa tataatacaa actataagat gttatcagta tttattatca
     9121 tttagaataa attttgtgtc gcccttaatt gtgagcggat aacaattacg agcttcatgc
     9181 acagtggcgt tgactcaata ttggccatta gccatattat tcattggtta tatagcataa
     9241 atcaatattg gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat
     9301 attggctcat gtccaatatg accgccatgt t
//
