LOCUS       sequence-385998-        9406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of secreted protein fused to 
            COMP5AP-AviTag-9xHis..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-SEMA3A-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3245..3280
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3290..3313
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4973..5017
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5018..5044
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5184..5201)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5190..5301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5419..5439)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5424..5753
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5604..5739
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5666..5685
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5801..5848
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5867..6466
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5867..5886)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6363..6383
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6596..6717
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6633..6652)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6687..6706
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6770..7561,7562..7630))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7393..7412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7631..7722)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7764..7783
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7789..7808
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7894..8482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8383..8402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8636..8653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8752..8768
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9224..9240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccaattacca gaacggcaag aacaacgtgc cccggctgaa
     1021 gctgagctac aaagagatgc tggaaagcaa caacgtgatc accttcaacg gcctggccaa
     1081 cagcagcagc taccacacct ttctgctgga cgaggaacgg tccagactgt acgtgggagc
     1141 caaggaccac atcttcagct tcgacctggt caacatcaag gacttccaga aaatcgtgtg
     1201 gcccgtgtcc tacaccagac gggatgagtg taaatgggcc ggcaaggaca tcctgaaaga
     1261 gtgcgccaac ttcatcaagg tgctgaaggc ctacaatcag acccacctgt acgcctgtgg
     1321 caccggcgct tttcacccta tctgcaccta catcgagatc ggccaccatc ctgaggacaa
     1381 tatcttcaag ctggaaaaca gccacttcga gaacggcaga ggcaagagcc cctacgatcc
     1441 taagctgctg acagccagcc tgctgatcga tggcgagctg tattctggca cagccgccga
     1501 cttcatgggc agagacttcg ccatcttcag aaccctgggc caccaccatc caatcagaac
     1561 cgagcagcac gacagccggt ggctgaacga ccctaagttt atcagcgccc acctgatcag
     1621 cgagagcgac aaccccgagg acgacaaggt gtacttcttc ttccgggaaa acgccatcga
     1681 cggcgagcac tctggaaaag ccacacacgc cagaatcggc cagatctgca agaacgactt
     1741 cggcggccac agatccctcg tgaacaagtg gaccaccttc ctgaaggccc ggctgatctg
     1801 ttctgtgccc ggacctaatg gcatcgacac ccacttcgac gagctgcagg acgtgttcct
     1861 gatgaacttc aaggacccca agaatcccgt ggtgtacggc gtgttcacca ccagcagcaa
     1921 catcttcaag ggcagcgccg tgtgcatgta cagcatgtcc gatgtgcgga gagtgtttct
     1981 gggcccctac gctcacagag atggccccaa ttatcagtgg gtgccctacc agggcagagt
     2041 gccctatcct agacctggca cctgtcctag caagaccttt ggcggcttcg acagcaccaa
     2101 ggacctgcct gacgatgtga ttaccttcgc cagatctcac cccgccatgt acaaccctgt
     2161 gttccccatg aacaacaggc ccatcgtgat caagaccgac gtgaactacc agttcaccca
     2221 gatcgtggtg gacagagtgg atgccgagga cggccagtac gacgtgatgt tcatcggcac
     2281 cgatgtgggc accgtgctga aagtggtgtc tatccccaaa gagacttggt acgacctgga
     2341 agaggtgctg ctggaagaga tgaccgtgtt cagagagccc acagccatca gcgccatgga
     2401 actgagcaca aaacagcaac agctgtacat cggcagcaca gccggcgttg cacaactgcc
     2461 tctgcacaga tgcgacatct acggcaaagc ctgcgccgag tgttgcctgg ccagagatcc
     2521 ttactgtgcc tgggatggca gcgcctgcag cagatacttt cctaccgcca agcggcggac
     2581 cagacggcag gatatcagaa acggcgaccc tctgacacac tgcagcgacc tgcaccacga
     2641 caatcaccac ggacacagcc ccgaggaaag aatcatctac ggggtcgaga actccagcac
     2701 attcctggaa tgcagcccca agagccagcg cgctctggtg tattggcagt tccagaggcg
     2761 caatgaggaa cggaaagagg aaatccgggt cgacgaccac atcatccgga cagatcaggg
     2821 cctgctgctg agaagcctgc aacagaagga cagcggcaac tacctgtgcc acgccgtgga
     2881 acacggcttc atccagacac tgctcaaagt gaccctggaa gtgatcgaca ccgagcacct
     2941 cgaggaactg ctgcacaagg acgatgatgg cgacggcagc aagaccaaag aaatgagcaa
     3001 cagcatgacg ccctctcaga aagtctggta cagggacttc atgcagctga tcaatcaccc
     3061 caacctgaac accatggacg agttctgcga gcaagtgtgg aagcgggaca gaaagcagcg
     3121 gagacagagg cctggacaca cccctggcaa tagcaacaaa tggaagcacc tccaagagaa
     3181 caagaaaggc cggaacagac ggacccacga gtttgagagg gcccctagat ctgttggcgc
     3241 gccggaagat caagttgatc caagattgat tgatggcaag ggcggatctc tggaggtgct
     3301 gttccaggga ccaggcggaa gcggcaatcc cggcggcgat ctggcccccc agatgctgcg
     3361 cgagctgcag gagaccaatg ccgccctgca ggacgtgcgc gagctgctgc gccaccgcgt
     3421 gaaggagatc accttcctga agaacaccgt gatggagtgc gacgcctgcg gtatgcagcc
     3481 ctctagaggc tccggctcca tcatcccagt tgaggaggag aacccggact tctggaaccg
     3541 cgaggcagcc gaggccctgg gtgccgccaa gaagctgcag cctgcacaga cagccgccaa
     3601 gaacctcatc atcttcctgg gcgatgggat gggggtgtct acggtgacag ctgccaggat
     3661 cctaaaaggg cagaagaagg acaaactggg gcctgagata cccctggcca tggaccgctt
     3721 cccatatgtg gctctgtcca agacatacaa tgtagacaaa catgtgccag acagtggagc
     3781 cacagccacg gcctacctgt gcggggtcaa gggcaacttc cagaccattg gcttgagtgc
     3841 agccgcccgc tttaaccagt gcaacacgac acgcggcaac gaggtcatct ccgtgatgaa
     3901 tcgggccaag aaagcaggga agtcagtggg agtggtaacc accacacgag tgcagcacgc
     3961 ctcgccagcc ggcacctacg cccacacggt gaaccgcaac tggtactcgg acgccgacgt
     4021 gcctgcctcg gcccgccagg aggggtgcca ggacatcgct acgcagctca tctccaacat
     4081 ggacattgac gtgatcctag gtggaggccg aaagtacatg tttcgcatgg gaaccccaga
     4141 ccctgagtac ccagatgact acagccaagg tgggaccagg ctggacggga agaatctggt
     4201 gcaggaatgg ctggcgaagc gccagggtgc ccggtatgtg tggaaccgca ctgagctcat
     4261 gcaggcttcc ctggacccgt ctgtgaccca tctcatgggt ctctttgagc ctggagacat
     4321 gaaatacgag atccaccgag actccacact ggacccctcc ctgatggaga tgacagaggc
     4381 tgccctgcgc ctgctgagca ggaacccccg cggcttcttc ctcttcgtgg agggtggtcg
     4441 catcgaccat ggtcatcatg aaagcagggc ttaccgggca ctgactgaga cgatcatgtt
     4501 cgacgacgcc attgagaggg cgggccagct caccagcgag gaggacacgc tgagcctcgt
     4561 cactgccgac cactcccacg tcttctcctt cggaggctac cccctgcgag ggagctccat
     4621 cttcgggctg gcccctggca aggcccggga caggaaggcc tacacggtcc tcctatacgg
     4681 aaacggtcca ggctatgtgc tcaaggacgg cgcccggccg gatgttaccg agagcgagag
     4741 cgggagcccc gagtatcggc agcagtcagc agtgcccctg gacgaagaga cccacgcagg
     4801 cgaggacgtg gcggtgttcg cgcgcggccc gcaggcgcac ctggttcacg gcgtgcagga
     4861 gcagaccttc atagcgcacg tcatggcctt cgccgcctgc ctggagccct acaccgcctg
     4921 cgacctggcg ccccccgccg gcaccaccga cgccgcgcac ccgggtggcg gaggactgaa
     4981 cgacatcttc gaggctcaga aaatcgaatg gcacgaacat caccatcacc atcaccatca
     5041 ccattaaggc cggcctgtag tcactggcac aacgcgttac ccggtaagcc aatcgggtat
     5101 acacggtcgt catactgcag acagggttct tctactttgc aagatagtct tgagtagtaa
     5161 aataaataga tagagaaaat cagcctcgac tgtgccttct agttgccagc catctgttgt
     5221 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     5281 ataaaatgag gaaattgcat cacaacactc aaccctatct cggtctattc ttttgattta
     5341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     5401 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     5461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     5521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     5581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     5641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     5701 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     5761 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     5821 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatga ccgagtacaa
     5881 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     5941 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     6001 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     6061 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     6121 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     6181 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     6241 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     6301 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga catccgcgcc
     6361 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     6421 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacacg tgctacgaga
     6481 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     6541 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     6601 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     6661 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     6721 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat taccaatgct
     6781 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6841 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agcgctgcga
     6901 tgataccgcg agaaccacgc tcaccggctc cggatttatc agcaataaac cagccagccg
     6961 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7021 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7081 tcgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     7141 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7201 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7261 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7321 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7381 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7441 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7501 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7561 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7621 gaatactcat attcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7681 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtc agtgttacaa
     7741 ccaattaacc aattctgaac attatcgcga gcccatttat acctgaatat ggctcataac
     7801 accccttgct catgaccaaa atcccttaac gtgagttacg cgcgcgtcgt tccactgagc
     7861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7921 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7981 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8041 tcttctagtg tagccgtagt tagcccacca cttcaagaac tctgtagcac cgcctacata
     8101 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8161 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8221 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8281 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8341 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8401 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8461 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8521 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8581 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8641 gtcagtgagc gaggaagcgg aaggcgagag tagggaactg ccaggcatca aactaagcag
     8701 aaggcccctg acggatggcc tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga
     8761 cggccagtct taagctcggg ccccctgggc ggttctgata acgagtaatc gttaatccgc
     8821 aaataacgta aaaacccgct tcggcgggtt tttttatggg gggagtttag ggaaagagca
     8881 tttgtcagaa tatttaaggg cgcctgtcac tttgcttgat atatgagaat tatttaacct
     8941 tataaatgag aaaaaagcaa cgcactttaa ataagatacg ttgctttttc gattgatgaa
     9001 cacctataat taaactattc atctattatt tatgattttt tgtatataca atatttctag
     9061 tttgttaaag agaattaaga aaataaatct cgaaaataat aaagggaaaa tcagtttttg
     9121 atatcaaaat tatacatgtc aacgataata caaaatataa tacaaactat aagatgttat
     9181 cagtatttat tatcatttag aataaatttt gtgtcgccct taattgtgag cggataacaa
     9241 ttacgagctt catgcacagt ggcgttgact caatattggc cattagccat attattcatt
     9301 ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat
     9361 aatatgtaca tttatattgg ctcatgtcca atatgaccgc catgtt
//
