LOCUS       sequence-385999-       10063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10063)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-CNTN1-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             3902..3937
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             3947..3970
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             5630..5674
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5675..5701
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(5841..5858)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5847..5958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6076..6096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6081..6410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6261..6396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6323..6342
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6458..6505
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6524..7123
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6524..6543)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7020..7040
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7253..7374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7290..7309)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7344..7363
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7427..8218,8219..8287))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8050..8069
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8288..8379)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8421..8440
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8446..8465
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8551..9139
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9040..9059
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9293..9310
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9408..9425
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9409..9425
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9881..9897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgagtttac ctggtataga agatatggcc acggcgtgtc
     1021 cgaagaggac aaaggctttg gccccatctt cgaggaacag cccatcaaca caatctaccc
     1081 cgaggaaagc ctggaaggca aggtgtccct gaactgcaga gccagagcct ctccatttcc
     1141 tgtgtacaag tggcggatga acaacggcga cgtggacctg accagcgaca gatatagcat
     1201 ggtcggaggc aacctggtca tcaacaaccc cgacaagcag aaggacgccg gcatctacta
     1261 ctgcctggcc agcaacaatt acggcatggt ccgaagcaca gaggccacac tgagcttcgg
     1321 ctatctggac ccatttcctc cagaggaacg gcccgaagtt cgcgtgaaag aaggcaaagg
     1381 catggtgctg ctgtgcgacc ctccttatca cttccccgac gacctgagct acagatggct
     1441 gctgaacgag ttccccgtgt tcatcaccat ggacaagcgg agattcgtgt cccagaccaa
     1501 cggcaacctg tatatcgcca acgtggaagc cagcgacaag ggcaactaca gctgcttcgt
     1561 gtctagcccc agcatcacca agagcgtgtt cagcaagttc atccctctga tccccattcc
     1621 tgagcggacc accaagcctt atcctgccga tatcgtggtg cagttcaagg atgtgtacgc
     1681 cctgatgggc cagaacgtga ccctggaatg tttcgccctg ggcaaccctg tgccagacat
     1741 tagatggcgg aaggtgctgg aacctatgcc tagcacagcc gagatctcta catctggcgc
     1801 cgtgctgaag atctttaaca tccagctcga ggacgagggc atctacgagt gcgaggccga
     1861 gaacatcaga ggcaaggata agcaccaggc cagaatctac gtgcaggcct ttcctgagtg
     1921 ggtcgagcac atcaacgaca ccgaagtgga catcggcagc gacctgtatt ggccttgtgt
     1981 ggccaccggc aagcccattc ctacaatccg gtggctgaag aacggctacg cctatcacaa
     2041 gggcgagctg agactgtacg acgtgacctt cgagaacgcc ggaatgtacc agtgtatcgc
     2101 cgagaatacc tacggcgcca tctacgccaa cgccgagctg aaaattctgg ccctggctcc
     2161 tacctttgag atgaacccca tgaagaagaa gatcctggcc gccaaaggcg gcagagtgat
     2221 catcgagtgc aagcccaaag ccgctcctaa gcctaagttc agctggtcca agggcaccga
     2281 gtggctggtc aatagcagca gaatcctgat ctgggaggac ggcagcctgg aaatcaacaa
     2341 catcaccaga aacgacggcg ggatctacac ctgtttcgcc gaaaacaacc gcggcaaggc
     2401 caactctaca ggcaccctcg tgatcacaga ccccaccaga atcatcctgg ctccaatcaa
     2461 cgccgacatc accgtgggcg agaatgccac aatgcagtgc gccgcctctt tcgaccctgc
     2521 tctggatctg accttcgtgt ggtccttcaa tggctacgtg atcgacttca acaaagagaa
     2581 catccactac cagcggaact tcatgctgga cagcaacggc gagctgctga tcagaaatgc
     2641 ccagctgaag cacgccggac ggtacacatg tacagcccag accatcgtgg ataacagcag
     2701 cgcctctgcc gatctggttg ttagaggacc tcctggacca cctggcggcc tgagaattga
     2761 ggatatcaga gccacaagcg tggccctgac atggtccaga ggcagcgata atcacagccc
     2821 catcagcaag tacaccatcc agaccaagac catcctgagc gacgactgga aggatgccaa
     2881 gaccgatcca cctatcatcg agggaaacat ggaagccgcc agagccgtgg atctgatccc
     2941 ttggatggaa tacgagttca gagtggtggc cactaacaca ctcggaagag gcgagcctag
     3001 catccccagc aacagaatca aaaccgacgg cgctgcccct aatgtggccc cttctgatgt
     3061 tggcggaggc ggcggaagaa acagagagct gacaattact tgggcccctc tgagcagaga
     3121 gtaccactac ggcaacaact tcggctacat cgtggccttc aagcccttcg acggcgagga
     3181 atggaagaaa gtgaccgtga ccaatcctga caccggcaga tacgtgcaca aggacgagac
     3241 aatgagcccc tccaccgcct tccaagtgaa agtgaaggcc tttaacaaca aaggcgacgg
     3301 cccttacagc ctggtggccg tgatcaattc tgcccaggat gctccttctg aggcccctac
     3361 tgaagtgggc gtgaaagtgc tgagcagcag cgagatttct gtgcactggg aacacgtgct
     3421 ggaaaagatc gtggaaagct accagatccg gtactgggcc gctcacgaca aagaagaggc
     3481 cgccaataga gtgcaagtga cgagccaaga gtacagcgcc aggctggaaa acctgctgcc
     3541 tgacacacag tacttcatcg aagtgggagc ctgcaatagc gccggatgtg gacctcctag
     3601 cgacatgatc gaggccttta ccaagaaggc ccctcctagc cagcctccta gaatcatcag
     3661 ctctgtcaga agcggcagcc ggtacatcat cacctgggat catgtggtgg ccctgtccaa
     3721 tgagagcacc gtgacaggct acaaggtgct gtacagaccc gatggccagc acgacggaaa
     3781 gctgtatagc acccacaagc acagcatcga ggtgcccatt ccaagagatg gcgagtacgt
     3841 ggtggaagtg cgggctcatt ctgatggcgg agatggggtc gtgtctcaag ttggcgcgcc
     3901 ggaagatcaa gttgatccaa gattgattga tggcaagggc ggatctctgg aggtgctgtt
     3961 ccagggacca ggcggaagcg gcaatcccgg cggcgatctg gccccccaga tgctgcgcga
     4021 gctgcaggag accaatgccg ccctgcagga cgtgcgcgag ctgctgcgcc accgcgtgaa
     4081 ggagatcacc ttcctgaaga acaccgtgat ggagtgcgac gcctgcggta tgcagccctc
     4141 tagaggctcc ggctccatca tcccagttga ggaggagaac ccggacttct ggaaccgcga
     4201 ggcagccgag gccctgggtg ccgccaagaa gctgcagcct gcacagacag ccgccaagaa
     4261 cctcatcatc ttcctgggcg atgggatggg ggtgtctacg gtgacagctg ccaggatcct
     4321 aaaagggcag aagaaggaca aactggggcc tgagataccc ctggccatgg accgcttccc
     4381 atatgtggct ctgtccaaga catacaatgt agacaaacat gtgccagaca gtggagccac
     4441 agccacggcc tacctgtgcg gggtcaaggg caacttccag accattggct tgagtgcagc
     4501 cgcccgcttt aaccagtgca acacgacacg cggcaacgag gtcatctccg tgatgaatcg
     4561 ggccaagaaa gcagggaagt cagtgggagt ggtaaccacc acacgagtgc agcacgcctc
     4621 gccagccggc acctacgccc acacggtgaa ccgcaactgg tactcggacg ccgacgtgcc
     4681 tgcctcggcc cgccaggagg ggtgccagga catcgctacg cagctcatct ccaacatgga
     4741 cattgacgtg atcctaggtg gaggccgaaa gtacatgttt cgcatgggaa ccccagaccc
     4801 tgagtaccca gatgactaca gccaaggtgg gaccaggctg gacgggaaga atctggtgca
     4861 ggaatggctg gcgaagcgcc agggtgcccg gtatgtgtgg aaccgcactg agctcatgca
     4921 ggcttccctg gacccgtctg tgacccatct catgggtctc tttgagcctg gagacatgaa
     4981 atacgagatc caccgagact ccacactgga cccctccctg atggagatga cagaggctgc
     5041 cctgcgcctg ctgagcagga acccccgcgg cttcttcctc ttcgtggagg gtggtcgcat
     5101 cgaccatggt catcatgaaa gcagggctta ccgggcactg actgagacga tcatgttcga
     5161 cgacgccatt gagagggcgg gccagctcac cagcgaggag gacacgctga gcctcgtcac
     5221 tgccgaccac tcccacgtct tctccttcgg aggctacccc ctgcgaggga gctccatctt
     5281 cgggctggcc cctggcaagg cccgggacag gaaggcctac acggtcctcc tatacggaaa
     5341 cggtccaggc tatgtgctca aggacggcgc ccggccggat gttaccgaga gcgagagcgg
     5401 gagccccgag tatcggcagc agtcagcagt gcccctggac gaagagaccc acgcaggcga
     5461 ggacgtggcg gtgttcgcgc gcggcccgca ggcgcacctg gttcacggcg tgcaggagca
     5521 gaccttcata gcgcacgtca tggccttcgc cgcctgcctg gagccctaca ccgcctgcga
     5581 cctggcgccc cccgccggca ccaccgacgc cgcgcacccg ggtggcggag gactgaacga
     5641 catcttcgag gctcagaaaa tcgaatggca cgaacatcac catcaccatc accatcacca
     5701 ttaaggccgg cctgtagtca ctggcacaac gcgttacccg gtaagccaat cgggtataca
     5761 cggtcgtcat actgcagaca gggttcttct actttgcaag atagtcttga gtagtaaaat
     5821 aaatagatag agaaaatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5881 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5941 aaatgaggaa attgcatcac aacactcaac cctatctcgg tctattcttt tgatttataa
     6001 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6061 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6121 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6181 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6241 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6301 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6361 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6421 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
     6481 gtatatcggc atagtataat acgacaaggt gaggaactaa accatgaccg agtacaagcc
     6541 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     6601 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     6661 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     6721 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     6781 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     6841 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     6901 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     6961 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacat ccgcgccccg
     7021 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7081 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacacgtgc tacgagattt
     7141 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     7201 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     7261 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7321 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7381 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcattac caatgcttaa
     7441 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7501 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagc gctgcgatga
     7561 taccgcgaga accacgctca ccggctccgg atttatcagc aataaaccag ccagccggaa
     7621 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7681 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccatcg
     7741 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7801 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7861 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7921 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7981 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8041 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8101 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8161 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8221 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8281 tactcatatt cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8341 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggtcagt gttacaacca
     8401 attaaccaat tctgaacatt atcgcgagcc catttatacc tgaatatggc tcataacacc
     8461 ccttgctcat gaccaaaatc ccttaacgtg agttacgcgc gcgtcgttcc actgagcgtc
     8521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     8701 tctagtgtag ccgtagttag cccaccactt caagaactct gtagcaccgc ctacatacct
     8761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9301 agtgagcgag gaagcggaag gcgagagtag ggaactgcca ggcatcaaac taagcagaag
     9361 gcccctgacg gatggccttt ttgcgtttct acaaactctt tctgtgttgt aaaacgacgg
     9421 ccagtcttaa gctcgggccc cctgggcggt tctgataacg agtaatcgtt aatccgcaaa
     9481 taacgtaaaa acccgcttcg gcgggttttt ttatgggggg agtttaggga aagagcattt
     9541 gtcagaatat ttaagggcgc ctgtcacttt gcttgatata tgagaattat ttaaccttat
     9601 aaatgagaaa aaagcaacgc actttaaata agatacgttg ctttttcgat tgatgaacac
     9661 ctataattaa actattcatc tattatttat gattttttgt atatacaata tttctagttt
     9721 gttaaagaga attaagaaaa taaatctcga aaataataaa gggaaaatca gtttttgata
     9781 tcaaaattat acatgtcaac gataatacaa aatataatac aaactataag atgttatcag
     9841 tatttattat catttagaat aaattttgtg tcgcccttaa ttgtgagcgg ataacaatta
     9901 cgagcttcat gcacagtggc gttgactcaa tattggccat tagccatatt attcattggt
     9961 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
    10021 atgtacattt atattggctc atgtccaata tgaccgccat gtt
//
