LOCUS       sequence-38600-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Vick JE, Johnson ET, Choudhary S, Bloch SE, Lopez-Gallego F, 
            Srivastava P, Tikh IB, Wawrzyn GT, Schmidt-Dannert C
  TITLE     Optimized compatible set of BioBrick vectors for metabolic pathway 
            engineering.
  JOURNAL   Appl Microbiol Biotechnol. 2011 Dec;92(6):1275-86. Epub 2011 Oct 28.
  PUBMED    22033566
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2011-12"; volume: "92(6)"; pages: 
            "1275-86. Epub 2011 Oct 28"
COMMENT     Sequence Label: pUCBB-pT7-eGFP
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             33..55
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    70..94
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(94..113)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(95..113)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      154..197
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(201..219)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        287..391
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(610..629)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctcgccctt gctcaccata tggatccaga tctctccttc ttaaagttaa acaaaattat
       61 ttctagaggg gaattgttat ccgctcacaa ttcccctata gtgagtcgta ttaatttcgc
      121 gggatcgtct agataagttc aggatgaatt ccagaaatca tccttagcga aagctaagga
      181 ttttttttat ctgaaattct gcctcgtgat acgcctattt ttataggtta atgtcatgat
      241 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      301 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
      361 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
      421 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
      481 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
      541 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
      601 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
      661 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
      721 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
      781 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
      841 gcacaacatg g
//
