LOCUS       sequence-386007-       12253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pMN-334
FEATURES             Location/Qualifiers
     source          1..12253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1002..1067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1642..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1679..1698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1733..1752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1944..2577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2624..2749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3246..3479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3664..3708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4179..4889)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4457..4476)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4726..4744
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4886..4895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5436..5843
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6426..6491
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6498..6518
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7368..7394
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7620..7991
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     misc_feature    8060..8648
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8113..8133)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8531..8542)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8651..8667)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8652..8668)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8758..8991
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(9016..9034)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9017..9034)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9324..9343)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9557..9576)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9676..9698
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9736..9754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9822..9926
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9927..9995,9996..10787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10145..10164)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10958..11546
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11447..11466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11700..11717
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11787..11807)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11792..12121
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11972..12107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
       61 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      121 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      181 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      241 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      301 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      361 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
      421 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
      481 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
      541 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
      601 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
      661 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
      721 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
      781 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
      841 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
      901 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
      961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     1141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     1201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     1261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     1321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     1381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     1441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     1501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1801 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1921 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1981 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2041 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2101 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     2161 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     2221 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2281 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2341 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2401 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2461 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2521 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2581 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2641 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2701 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2761 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2821 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2881 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2941 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3001 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3061 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     3121 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3181 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3241 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3301 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3361 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3421 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3481 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3541 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3601 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3661 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3721 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3781 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3841 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3901 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3961 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 gtttggatcg ataagcttga tatcgaattc ctgcagcctt acttgtacag ctcgtccatg
     4201 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     4261 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
     4321 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
     4381 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
     4441 gcctgccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
     4501 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     4561 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
     4621 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
     4681 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     4741 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     4801 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
     4861 atgttatcct cctcgccctt gctcaccatg gtggcgatcc ggtagcgcta gcgatgagct
     4921 ctggtagtag actagtggcc cccattatat accctctaga ggatcctatc cactccgtgt
     4981 aagggagagt gagcctctta cgaatgcgcg acatcggcta cgccgttccg aggcgactga
     5041 tacgcgcgac atcggctacg ccgtacgaag gcagtccgat tgcgcgacat cggctacgcc
     5101 gacctttact gagacgggag cgcgacatcg gctacgccga aggcgttgcg aatcctcatg
     5161 cgattgttac gaaacccggc gcgacatcgg ctacgccgta ttggaagcac ggctctgcgc
     5221 gacatcggct acgccgacct cgggagaact gattgcgcga catcggctac gccgaagagg
     5281 ttgacgctat ccgcgcgaca tcggctacgc cgagccctgt aggtagacta gatacagtgt
     5341 tactcaatcg tccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     5401 aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga
     5461 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     5521 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     5581 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     5641 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     5701 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     5761 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     5821 tccgacagac tgagtcgccc gggggggatc tggagctctc gagaattctc acgcgtgccg
     5881 ccaccatgtc tagacccgga gagcgcccat tccagtgtcg gatttgcatg cggaactttt
     5941 cgagaagaca cggcctggac agacataccc gtactcatac aggtgaaaaa ccctttcagt
     6001 gtcggatctg tatgcgaaat ttctccgacc acagcagcct gaagagacat ctacgtaccc
     6061 acaccggcag ccagaagcca tttcagtgtc ggatctgtat gcggaacttc tccgtgagac
     6121 acaacctgac cagacatcta cgtacgcaca ccggagagaa gccattccaa tgccgaatat
     6181 gcatgcgcaa cttcagtgac cacagcaacc tgagcagaca cctaaaaacc cacaccggtt
     6241 cccagaagcc atttcagtgt cggatctgta tgcggaactt ctcccagcgc agcagcctgg
     6301 tgagacatct acgtacgcac accggagaga agccattcca atgccgaata tgcatgcgca
     6361 acttcagtga gagcggccac ctgaagagac acctgcgtac gcacctgagg ggatccacct
     6421 gcagggacta caaagaccat gacggtgatt ataaagatca tgacatcgat tacaaggatg
     6481 acgatgacaa gatggccccc aagaaaaaga ggaaggtggg cattcacggg gtgccgggtg
     6541 gactcgaggg aggcggtgga agcggcggta ccgaggacgt ggtgtgctgc cactcaatct
     6601 acggcaagaa gaagggtgat atcgacacct accgatacat aggctcttcc gggacaggct
     6661 gcgtggtcat agtgggcagg atcgtcttgt ccggatccgg cactagtgcg cccatcacgg
     6721 cgtacgccca gcagacgaga ggcctcctag ggtgtataat caccagcctg actggccggg
     6781 acaaaaacca agtggagggt gaggtccaga tcgtgtcaac tgctacccaa accttcctgg
     6841 caacgtgcat caatggggta tgctgggcag tctaccacgg ggccggaacg aggaccatcg
     6901 catcacccaa gggtcctgtc atccagatgt ataccaatgt ggaccaagac cttgtgggct
     6961 ggcccgctcc tcaaggttcc cgctcattga caccctgtac ctgcggctcc tcggaccttt
     7021 acctggtcac gaggcacgcc gatgtcattc ccgtgcgccg gcgaggtgat agcaggggta
     7081 gcctgctttc gccccggccc atttcctact tgaaaggctc ctcggggggt ccgctgttgt
     7141 gccccgcggg acacgccgtg ggcctattca gggccgcggt gtgcacccgt ggagtggcta
     7201 aagcggtgga ctttatccct gtggagaacc tagagacaac catgagatcc ccggtgttca
     7261 cggacaactc ctctccacca gcagtcaccc tgacgcaccc aatcaccaaa atcgataggg
     7321 aggttctcta ccaggagttc gatgagatgg aagagtgctc tcagcactat ccctacgatg
     7381 tgcccgatta cgctggaggc ggtggaagcg gcggtaccga tgagtttccc accatggtgt
     7441 ttccttctgg gcagatcagc caggcctcgg ccttggcccc ggcccctccc caagtcctgc
     7501 cccaggctcc agcccctgcc cctgctccag ccatggtatc agctctggcc caggccccag
     7561 cccctgtccc agtcctagcc ccaggccctc ctcaggctgt ggccccacct gcccccaagc
     7621 ccacccaggc tggggaagga acgctgtcag aggccctgct gcagctgcag tttgatgatg
     7681 aagacctggg ggccttgctt ggcaacagca cagacccagc tgtgttcaca gacctggcat
     7741 ccgtcgacaa ctccgagttt cagcagctgc tgaaccaggg catacctgtg gccccccaca
     7801 caactgagcc catgctgatg gagtaccctg aggctataac tcgcctagtg acaggggccc
     7861 agaggccccc cgacccagct cctgctccac tgggggcccc ggggctcccc aatggcctcc
     7921 tttcaggaga tgaagacttc tcctccattg cggacatgga cttctcagcc ctgctgagtc
     7981 agatcagctc ccaattgtaa gcggccgcga ctctagagtc gacctgcagg catgcaagct
     8041 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8101 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8161 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8221 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8281 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8341 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     8401 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     8461 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     8521 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     8581 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     8641 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     8701 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     8761 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     8821 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     8881 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     8941 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     9001 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     9061 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     9121 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     9181 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     9241 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     9301 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     9361 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     9421 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     9481 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     9541 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     9601 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     9661 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     9721 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     9781 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9841 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     9901 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9961 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10021 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10081 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10141 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10201 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10261 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10321 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10381 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10441 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10501 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10561 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10621 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10681 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10741 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    10801 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    10861 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    10921 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10981 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11041 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11101 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11161 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11221 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11281 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11341 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11401 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11461 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11521 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11581 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11641 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11701 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    11761 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    11821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    11881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    11941 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    12001 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    12061 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    12121 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
    12181 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
    12241 cagatctcta taa
//
